梗阻性黄疸大鼠血浆NO与ET改变及其意义

目的 探讨大鼠梗阻性黄疸时血浆ＮＯ和ＥＴ的改变及其意义。方法 胆总管结扎法建立梗阻性黄疸模型,用放射免疫分析法和比色法测定血浆ＮＯ和ＥＴ的水平,生理记录仪测定心率（ＨＲ）、平均动脉压（ＭＡＰ）、心输出量（ＣＯ）。结果 胆总管结扎组（ＢＤＬ组）大鼠血浆ＮＯ含量明显增高,ＥＴ含量下降,平均动脉压（ＭＡＰ）下降,心率（ＨＲ）减慢,心输出量（ＣＯ）增加,与ＳＯ组、Ｎ组相比,ＳＯ组与Ｎ组之间差异不明显。结论     

内镜下切除胃肠道息肉及癌前病变方法的选择

目的;比较不同方法切除胃肠道息肉和癌前病变的疗效。方法：本文采用高频电和其它不同方法对胃肠道息肉和癌前病变切除和切割。结果：对小的扁平息肉用高频电灼除或热活检安全有效。一次的灼除达100％。圈套适应胃结肠有蒂息肉。对大的有蒂或分叶状息肉需分次圈套切除,圈套和烧灼或热活检结合可避免肠穿孔发生。双胃镜代替双腔治疗胃镜行粘膜切除胃内平坦和扁平隆起性非典型增生灶操作容易,病灶切除彻底。息肉切除后常于局部形成溃疡,术后应常规给予酸抑制剂和粘膜保护剂治疗。结论：内镜－高频电是切除胃肠道有蒂和无蒂息肉的有效方法,粘膜染色后,双胃镜行粘膜和术治疗胃内癌前病变切实可行。     

477例患者血液透析中留置中心静脉导管的临床分析

目的：总结留置中心静脉导管的经验,探讨如何防治中心静脉留置导管的并发症。方法：分析１９９２年７月！￣１９９８年７月我院血液净化中心对４７７例肾功能不全患,留置中心静脉导管共５６６例次。     

产时保健对母婴健康的影响

目的：了解产妇及胎儿在医院分娩过程中产时保健情况对母婴健康的影响。方法：对１１６６４例产妇接产情况,采用χ＾２检验对不同年份不同分娩方式及母婴分娩并发症发生率进行对比分析。结果：剖宫产率为４１．８４％,围产儿死亡率为１０．６８‰,产妇分娩并发症的发生率为５．９９％,其中产后出血占９５．４２％,剖宫产与阴式分娩产后出血率差异无显著性（χ＾２＝０．６６,Ｐ＞０．０５）,新生儿并发症发生率为８．５８％,     

重型颅脑损伤66例死因分析

回顾分析２３６例重型颅脑损伤患者中６６例死亡病人的临床资料,结果显示,受伤原因中车祸伤占４５．８％,堕落伤占２４．２％,跌伤占１８．１％,ＣＴ扫描见脑挫裂伤并颅内血肿占６３．６％,单纯脑挫裂伤和颅内血肿各占９．０％,２７．３％。采用亚低温加常规综合治疗后死亡率仅为１８．２％,而非亚低温加常规综合治疗死亡率为２９．２％。     

吗啡依赖动物戒断后的焦虑情绪变化

本文采用高架十字迷宫模型和Vogel's饮水冲突模型观察吗啡依赖动物自然戒断后18、24、48、72、96、120h的焦虑情绪 ,结果表明吗啡依赖动物自然戒断后48h时的焦虑症状在两种模型上都最为明显 ,丁螺环酮可明显缓解吗啡所致的焦虑情绪。提示高架十字迷宫模型和Vogel's饮水冲突模型可较为客观和准确地反映吗啡所致焦虑情绪。     

中药地龙的药源调查与商品鉴定

经对我国地龙药材的药源调查与商品鉴定,结果表明,地龙的原动物主要有１３种和变种。分别隶属于矩蚓蝌的３个属,其中９０％以上的商品地龙来源于药典收载品种参环毛蚓Ｐｈｅｒｅｔｉｍａ ａｓｐｅｒｇｉｌｌａｍ和威廉环毛蚓Ｐ．ｇｕｉｌｌｅｌｍｉ、栉盲环毛ｐｅｃｔｉｎｉｆｅｒａ及通俗环毛．ｖｕｌｇｏｒｉｓ其它品种如背暗异唇Ａｌｌｏｌｂｏｐｈｏｒａ ｃａｌｉｇｉｎｏｓａ ｔｒａｐｒｚｏｉｄｅｓ、湖北环毛蚓Ｐ、ｈｕ     

The effect of TGFβ1 on murine tumor growth following direct intratumoral injection of plasmid DNA

In order to investigate TGFβ1 gene expression and its effect on murine tumor growth following direct intratumoral injection of naked plasmid DNA encoding human TGFβ1, LM₃ murine lung adenocarcinoma cells were inoculated subcutaneously to T₇₃₉ mice and grew to tumor nodules in 2 weeks. Multiple direct intratumoral injection of plasmid DNA, PMAMneo- TGFβ1, were given and compared with saline or vector plasmid administration groups. The growth of tumor was observed till the 8th week when the mice were killed for Northern blot analysis and histopathological study of tumoral tissue. The results showed that the growth of tumor was boosted in the TGFβ1 gene treated mice as compared with the control groups, whereas there was no significant difference in the metastatic behavior. Northern blot showed efficient expression of TGFβ1 mRNA in the treated group. The present study indicated that TGFβ1 may stimulate tumor growthin vivo through certain mechanisms. And direct intra-tumoral injection of nude plasmid DNA may be a promising gene transfer strategyin vivo. This work was supported by the National Natural Science Foundation of China (No. 39370761).     

Rest and treadmill exercise first-pass radionuclide ventriculography: Validation of left ventricular ejection fraction measurements

Background

To assess the accuracy of rest and treadmill exercise first-pass radionuclide ventriculographic measurements of left ventricular ejection fraction (LVEF), 40 patients underwent treadmill exercise first-pass and bicycle exercise equilibrium radionuclide ventriculography. To determine the frequency of technically adequate treadmill exercise first-pass studies, an additional 128 consecutive patients undergoing treadmill exercise first-pass procedures during stress^99mTc-labeled sestamibi myocardial perfusion studies were assessed.

Methods and Results

The treadmill exercise first-pass procedure used a multicrystal camera and an²⁴¹Am point source to allow for correction of patient motion. Excellent correlations were observed between resting first-pass and resting equilibrium LVEF (r=0.91; standard error of the estimate=5.6). A high correlation was also observed between treadmill exercise first-pass and bicycle equilibrium exercise LVEF measurements (r=0.85, standard error of the estimate=7.6). Treadmill first-pass LVEF systematically underestimated the bicycle equilibrium LVEF. Intraobserver agreement for rest and exercise first-pass LVEF was high (r values of 0.98 and 0.94, respectively). Of the 168 consecutive treadmill exercise first-pass studies evaluated for technical adequacy, 21 (12.5%) were deemed technically inadequate, with most of the sources of error being avoidable. The frequency of technically adequate studies was as high (87%) in high levels of exercise (Bruce stages 3 and 4) as in lower levels (88%). The findings of this study validate the first-pass treadmill exercise LVEF measurement.

Conclusion

This procedure now provides the option for combining the information of peak treadmill exercise LVEF with measurements of exercise myocardial perfusion from the same injection of radioactivity.     

肝癌介入治疗药动学及其临床意义探讨

本研究通过测定血清阿霉素浓度,分析了原发性肝癌阿霉素肝动脉灌注(A 组)、阿霉素碘油乳化后栓塞(B 组)及阿霉素微囊栓塞化疗(C 组)后药动学变化及其临床意义。A 组药动学变化与静脉给药相似,但峰值浓度高。B 组峰浓度低,半衰期延长,显示缓释和降低血浓度的作用。C 组峰浓度不明显,表现为长时、稳定的释放,血浓度低,具有保持局部长时间高浓度作用。结果揭示了肝癌介入治疗不同给药方法疗效差异的药动学依据。