临床超声技术在腹部淋巴结病变检查治疗中的应用

目的　研究分析腹部淋巴结主要病变的超声声像图特征及超声引导活检的临床应用价值。方法　对腹部淋巴结病变进行超声检查和超声引导活检。结果　超声检查可准确显示腹部淋巴结病变的数目、大小、形态、部位及其内部结构 ,能提示与周围组织的关系 ,从而作出诊断与鉴别诊断 ;超声引导能成功进行穿刺活检。结论　超声检查和超声引导活检是诊断腹部淋巴结病变的一种简便、快捷 ,准确、实用的检查手段 ,对明确临床诊断 ,指导临床治疗和观察病变进展与临床疗效 ,均具有良好的应用价值。     

Up-regulation of Niacinamide in Intervertebral Disc Aggrecan in vitro

The regulatory effects of niacinamide （Nia） on intervertebral disc （IVD） aggrecan in vitro was investigated. Chiba＇s 10 ng/mL interleukin-1 （IL-1）-induced rabbit IVD degeneration model in vitro was established. 0.5, 0. 25 and 0.05 mg/mL Nia was added to normal and degenerated IVDs for intervention. On the first and second week after intervention, safranin O-fast green staining intensity and glycosaminoglycan （GS） content were measured. The expression of aggrecan core protein was detected by RT-PCR. The results showed： （1） After treatment with 0. 5 mg/mL Nia for one week, the GS content in nucleus pulposus （NP） was increased by 44.80% as compared with control group （P〈0. 01） ; The GS content in IL-1 induction groups was increased with the increase of Nia concentrations： After treatment with 0. 5 mg/mL for one week, the GS content in NP was increased by 68.30% as compared with control group （P〈0. 01）. After two weeks, GS content in NP and fibrous rings was still higher than in control group at the same period （P〈0. 01） and untreated group （P〈0.01）. （2） Safranin O-fast green staining revealed that with the increase of Nia concentrations, staining density in NP and fibrous rings was increased and histological structure damage to IVDs by IL-1β was alleviated. （3） RT-PCR showed that the expression of core protein gene in IL-1β-induced degenerated IVDS was increased with the increase of Nia concentrations. It was concluded that under conditions in vitro, Nia could up-regulate the expression of aggrecan in IVDs and protect IVDs from IL-1β-induced degeneration at least partially, which offers a potential choice for IVD degeneration clinical therapy.     

Brief Introduction to Digital Acupoint Pressure Therapy

The Concept of Digital Acupoint Pressure Therapy The digital acupoint pressure therapy means that according to different diseases and the illness condition, the physician strikes, presses or taps the acupoints with hands on the patient＇s body surface to promote the circulation of qi and blood, thus making dysfunctions of certain organs or the affected areas return to normal.     

How to Treat Hyperplasia of Prostate Based on TCM Differentiation

Hyperplasia of prostate, a frequently encountered disease often affecting males over 50 years old, belongs to the category of urinary retention in TCM. Its pathogenesis is deficiency of the kidney and accumulation of heat in the lower-jiao, which may lead…     

中西医结合治疗急性重症胰腺炎临床体会

目的探讨分析中西医结合治疗急性重症胰腺炎的疗效以及可行性。方法回顾分析我院1999年9月￣2005年9月收治的行中西医结合治疗的急性重症胰腺炎病人72例的治疗情况,与以往行手术治疗的治疗效果进行比较。结果治愈57例,治愈率79.2%,中转手术8例,死亡7例,死亡率9.7%,并发症发生率18%。结论中西医结合方法治疗急性重症胰腺炎的效果良好,可明显降低并发症发生率及死亡率。     

儿童伤害的流行现状与预防控制

凡由于能量(机械能、热能、化学能等)的传递或干扰超过人体的耐受性造成组织损伤,或窒息导致缺氧,影响了正常活动,需要医治或看护,称之为伤害[1]。伤害按其意图可分为意外伤害和故意伤害,常见的意外伤害包括道路交通伤害、溺水、跌伤、中毒和烧烫伤;常见的儿童故意伤害包括儿童     

铅对小鼠睾丸TGFβ1及Caspase-3表达的影响

目的 探讨不同浓度和时间醋酸铅染毒对小鼠睾丸转化生长因子β1（Transforming growth factor β1，TGFβ1）、半胱天冬酶3（Caspase-3）表达的影响，进一步阐明醋酸铅所致睾丸毒性的作用机制。方法 45只雄性小鼠分为空白对照组和0．2％，0．4％醋酸铅染毒组，每组15只。并在第2，4，6周分别处死每组小鼠各5只，取其睾丸组织，免疫组化检测TGFβ1、Caspase-3表达的差异。结果 染铅后不同周TGFβ1、Caspase-3的表达较空白对照组增强，差异有统计学意义（P〈0．05）。且随着染毒时间的延长，表达越强（P〈0．05）。但在0．2％与0．4％不同周染铅组之间TGFβ1、Caspase-3的表达差异并无统计学意义（P〉0．05）。结论 醋酸铅使睾丸TGFβ1 Caspse-3的表达增强，从而促使生殖细胞发生凋亡。     

宫腔镜临床应用及相关问题讨论

主要讨论内容 1．官腔镜临床应用现状及其发展趋势 2．子宫内膜息肉手术指证及相关问题 3．功能失调性子宫出血的宫腔镜治疗及其注意事项 4．子宫畸形的宫腔镜矫治 5．官腔镜用于诊断早期子宫内膜癌的价值及其对预后的影响 6．官腔镜手术灌流液过量吸收并发症的预防     

医院PACS系统的效益研究

PACS的建设是当前医院信息化建设的热点。文章根据在大型三甲医院实际评测的数据和效果,指出PACS在节约医院成本、提高诊断质量和医疗效率等方面为医院带来重要效益。     

局部亚低温对大鼠脑出血后水通道蛋白4表达的影响

目的观察局部亚低温对大鼠自体血注入法脑出血模型水通道蛋白4(AQP-4)mRNA及蛋白质表达的影响,探讨局部亚低温减轻脑出血后水肿的可能机制。方法雄性W istar大鼠240只,随机分为脑出血(ICH)组和脑出血加局部亚低温(ICH+H)组。每组分为对照、脑出血后6、24、72 h,5、7 d共6个亚组,ICH+H组于注血后给予4 h的局部亚低温治疗,各亚组分别进行血脑屏障(BBB)通透性、脑水含量的检测以及应用逆转录(RT)-PCR及W estern印迹对AQP-4进行测定。结果ICH组大鼠脑组织水含量、BBB通透性以及AQP-4 mRNA表达的增加始于脑出血后6 h,AQP-4蛋白质表达的增加始于脑出血后24 h,均至72 h达高峰(P<0.01)。AQP-4 mRNA及蛋白质表达的变化与BBB通透性的变化呈正相关(r=0.78和r=0.76)。ICH+H组的脑组织水含量和BBB通透性在各时间点与ICH组相比明显下降,而AQP-4 mRNA吸光度值(A)在脑出血后48 h与ICH组相比开始较低,在72 h,由ICH组的1.25±0.03降至1.04±0.02(P<0.01),AQP-4蛋白质A值在各时间点与ICH组相比明显下降,在72 h,由ICH组的0.77±0.08下降至0.25±0.04(P<0.01)。结论脑出血后BBB完整性的破坏可以导致AQP-4表达的上升。局部亚低温可以抑制脑出血后AQP-4 mRNA和蛋白质表达的增加。局部亚低温可能既通过减轻BBB完整性的破坏在转录水平抑制AQP-4 mRNA表达的增加,也可以在翻译水平直接抑制AQP-4蛋白质的表达,来减轻脑出血后的脑水肿形成。