Roles of AKAP1 in high-glucose induced mitochondrial fission in podocytes

Objective To investigate the roles of A kinase anchoring protein1(AKAP1)in high-glucose induced mitochondrial fission in podocytes. Methods Conditionally immortalized human podocytes were cultured in serum-free medium for 24 hours, and then exposed to different glucose concentration conditions in different time periods. The protein expressions of AKAP1 were observed by immunofluorescence, and AKAP1, dynamin related protein1 (Drp1) and phospho Ser 637-Drp1 (p-Drp1) were analyzed by Western blotting. AKAP1 siRNA was transfected to block AKAP1 expression.Podocytes were then divided into normal control group (5 mmol/L glucose), hypertonic group (30 mmol/L mannitol+5 mmol/L glucose), high glucose group (35 mmol/L glucose), and high glucose+AKAP1 siRNA group. Mitochondrial morphological changes were assessed by mitotracker red staining. Podocyte apoptosis was assessed by flow cytometry. Results Compared with normal group, high-glucose induced more podocytes apoptosis (P＜0.05), more mitochondrial fission with decreased aspect ratio and form factor (all P＜0.05). Upregulated AKAP1 protein level, and increased ratio of p-Drp1/Drp1 (all P＜0.05) in time and concentration dependent manners were also observed. Compared with high glucose group, transfection of AKAP1 siRNA showed less apoptosis (P＜0.05), less mitochondrial fission with increased aspect ratio and form factor (all P＜0.05), and down-regulated AKAP1 protein level as well as p-Drp1/Drp1 ratio (all P＜0.05). Conclusion High glucose induced mitochondrial fission might be induced through AKAP1-Drp1 pathway.     

胃癌根治术后非计划再次手术的原因和危险因素分析

目的通过对病例资料的回顾性研究分析胃癌根治术后非计划再次手术的原因和危险因素。方法回顾性分析2012年1月至2017年12月在南京大学医学院附属鼓楼医院行胃癌根治术(包括开腹和腹腔镜)的病人资料,纳入标准:(1)年龄18～80岁;(2)胃镜病理明确诊断胃癌;(3)接受D2胃癌根治术(包括开腹和腹腔镜手术)。排除标准:(1)胃癌术中行联合脏器切除术;(2)残胃癌行胃癌根治术。共统计胃癌手术病人3 213例,其中接受非计划再次手术病人37例。按照性别、年龄进行1∶2配对,以胃癌术后未接受再次手术的病人为对照组,开展回顾性配对研究,查找非计划再次手术的原因,同时分析导致发生非计划再次手术的危险因素。结果术后行非计划再次手术的中位时间为1d(0～9d),开腹术后31例,腹腔镜术后6例。手术原因包括:腹腔出血(23例,62.2%);吻合口出血(3例,8.1%);吻合口漏(3例,8.1%);切口裂开(2例);肠梗阻(2例);十二指肠残端漏(1例);急性胆囊炎(1例);脾梗死(1例);切口感染(1例)。单因素分析结果显示,非计划再次手术组与对照组的病人在术中出血量(P=0.014)、第一助手资格(P=0.042)方面差异有统计学意义,手术时间、主刀医生资格、手术开始时间、术中输血、手术方式、手术部位等方面差异无统计学意义。多因素Logistic回归分析显示:术中出血量≥200ml是非计划再次手术的独立危险因素(OR=1.827,95%CI:1.034,3.026,P=0.024)。结论腹腔出血是胃癌根治术后非计划再次手术的最常见原因,术中出血量≥200ml是非计划再次手术的独立危险因素。     

高龄初产妇剖宫产后宫缩乏力性出血的高危因素及干预措施

目的研究高龄初产妇剖宫产后宫缩乏力性出血的高危因素及干预措施。方法回顾性选取2021年1月至2022年1月于潍坊市脑科医院就诊、行剖宫产具有产后出血高危因素的161例高龄初产妇为研究对象, 年龄30～46(37.23±2.33)岁, 体质量指数(BMI)18～25(22.52±1.47)kg/m2。依据有无宫缩乏力性产后出血将其分为宫缩乏力性产后出血组(38例), 无宫缩乏力性产后出血组(123例)。对临床资料进行整理并采用单因素分析, 之后多因素logistic回归分析将其中差异有统计学意义的因素进行筛选, 筛选出相关危险因素。采用t检验、χ2检验。结果 161例高龄初产妇中, 宫缩乏力性产后出血组宫腔手术史[78.95%(30/38)]、妊娠期高血压疾病[73.68%(28/38)]、新生儿体质量≥4 kg[89.47%(34/38)]、前置胎盘[78.95%(30/38)]、焦虑或抑郁病史初产妇比例[55.26%(21/38)]均高于无宫缩乏力性产后出血组[36.59%(45/123)、33.33%(41/123)、54.47%(67/123)、39.02%(48/123)、34...     

Feeding on different attractive flowering plants affects the energy reserves of Culex pipiens pallens adults

Parasitology Research - Mosquito adults usually need to ingest sugar from nectar host plants to sustain their metabolic needs. Mosquitoes could be differentially attracted by various flowering...