雷公藤多甙对局灶性脑缺血再灌注大鼠脑组织单胺类递质含量的影响

目的：观察雷公藤多甙（TWP）对大鼠局灶性脑缺血再灌注损伤脑组织单胺类神经递质含量的影响.方法：Wistar大鼠60只,随机均分为6组.假手术组、缺血再灌注组每d灌胃生理盐水,尼莫地平（Nim）组、雷公藤多甙高、中、低剂量组,分别给予Nim 10 mg/kg,TWP 45 mg/kg、30 mg/kg、15 mg/kg,灌胃给药,1次/d,连用5 d.末次给药后1 h,采用线栓法制作大鼠大脑中动脉局灶性缺血再灌注模型,用荧光分光光度计测定缺血2 h再灌注1 h时各组大鼠大脑皮层及纹状体去甲肾上腺素（NE）、多巴胺（DA）、5-羟色胺（5-HT）含量.结果：缺血再灌注组大鼠大脑皮层及纹状体NE、DA、5-HT含量均较假手术组降低;与缺血再灌注组比较,雷公藤多甙各组及尼莫地平组大鼠大脑皮层及纹状体NE、DA、5-HT含量均升高（P＜0.05）.结论：雷公藤多甙可减少缺血再灌注期间单胺类神经递质的释放.     

miR-125b confers resistance of ovarian cancer cells to cisplatin by targeting pro-apoptotic Bcl-2 antagonist killer 1

Chemotherapy is the preferred therapeutic approach for advanced ovarian cancer,but a successful long-term treatment is prevented by the development of drug resistance.Recent works have underlined the involvement of non-coding RNAs,microRNAs(miRNAs) in cancer development,with several conjectures regarding their possible involvement in the evolution of drug resistance.This study is to investigate the promoting effects and mechanism of miR-125b involved in the development of chemoresistance in ovarian cancer.The different expression of miR-125b in cisplatin-sensitive ovarian cancer cell line(OV2008) and its resistant variant(C13*) was identified by real-time PCR.An in vitro cytotoxicity assay and apoptosis assay using CCK-8 assay and flow cytometry,were carried out to detect the effect of miR-125b and Bak1 on cisplatin resistance of cells.Real-time PCR,Western blotting and luciferase reporter assay were used to detect whether Bak1 is a target of miR-125b.As compared with OV2008 cells,the expression levels of miR-125b in C13* cells were increased.It was found that the up-regulation of microRNA-125b caused a marked inhibition of cisplatin-induced cytotoxicity and apoptosis and a subsequent increase in the resistance to cisplatin in OV2008 and C13* cells.Moreover,Bak1 was a direct target of miR-125b,and down-regulation of Bak1 suppressed cisplatin-induced apoptosis and led to an increased resistance to cisplatin.Our study indicates that miR-125b has a significantly promoting effect on chemoresistance of C13* cells and up-regulation of miR-125b expression contributes to cisplatin resistance through suppression of Bak1 expression.This finding has important implications in the development of targeted therapeutics for overcoming cisplatin resistance in ovarian cancer.     

灯盏细辛注射液与灯盏生脉胶囊治疗缺血性中风上市后临床再评价

目的:验证灯盏细辛注射液和灯盏生脉胶囊(以下称灯盏组)上市后治疗缺血性中风的疗效和安全性。方法:采用多中心、前瞻性、实用性随机对照研究设计,应用"临床研究中央随机系统",对678例患者进行随机分组,灯盏组343例,西医组335例。灯盏组急性期以灯盏细辛注射液治疗为主,恢复期以口服灯盏生脉胶囊为主。疗效评价指标主要为随访360 d的重要临床结局事件(病死率、复发率、致残率、生存质量)。安全性评价主要观察实验室检查指标、不良事件及发生率。结果:随访360 d,灯盏组病死4例,病死率为1.17%,西医组病死16例,病死率为4.78%,灯盏组病死率显著低于西医组(P<0.05);灯盏组复发11例,复发率为3.21%,西医组复发12例,复发率为3.59%,灯盏组复发率略低于西医组;灯盏组致残率为39.53%,其中严重致残率为1.49%,西医组的致残率为40.13%,其中严重致残率为3.13%,灯盏组致残率与残疾严重程度均低于西医组;生存质量比较,灯盏组对脑卒中患者活动能力、上肢功能评分方面显著优于西医组(P<0.05)。安全性分析显示,灯盏组发生不良事件11例,与药物治疗相关为4例,不良反应发生率1.17%,主要表现为发热寒战、皮疹、恶心、头晕心慌,全部为使用灯盏细辛注射液后出现,停药后1～2 d症状消失。灯盏组中发生13例肝功能异常,2例肾功能异常,临床医生判断3例ALT异常可能与药物使用有关,其余均与药物无关。结论:灯盏细辛注射液与灯盏生脉胶囊是治疗缺血性中风安全有效的中药。     

CDMA和PCR-SSP方法对HLA-B_(27)分型的探讨

目的了解补体依赖性微量淋巴细胞毒实验(CDMA)对人类白细胞抗原B27(HLA-B27)检测结果情况。方法采用聚合酶链反应-序列特异性引物(PCR-SSP)实验作为参考实验,对用CDMA方法检测过的标本用PCR-SSP方法进行复检,比较2种检测结果。结果用2种方法对216例标本进行检测,其中212例标本结果一致,4例标本CDMA为阴性而PCR-SSP为阳性,经统计学分析,差异无统计学意义(χ2=2.25,0.1相似文献   

苯海索对血红素所致大鼠胚胎皮层神经细胞损伤的保护作用

目的 :观察苯海索对血红素所致大鼠胚胎皮层神经细胞损伤的保护作用。方法 :体外培养大鼠胚胎皮层神经细胞 ,加入血红素建立神经细胞血红素损伤模型 ,以不加血红素者为正常对照 ,观察不同浓度苯海索对血红素损伤模型神经细胞死亡数 ,乳酸脱氢酶 (LDH)的漏出量 ,培养液中丙二醛 (MDA)和超氧化物歧化酶 (SOD)活性的影响。结果 :苯海索 10 -9mol·L-1、10 -8mol·L-1、10 -7mol·L-1能浓度依赖性地显著减少细胞死亡 ,降低LDH漏出量及MDA生成 ,提高SOD活性。结论 :苯海索对血红素所致神经细胞损伤具有显著的保护作用 ,其机制可能与苯海索抗过氧化作用有关。     

Effect of the cyclin-dependent kinases inhibitor p27 on resistance of ovarian cancer multicellular spheroids to anticancer chemotherapy

Purpose: A low proliferating fraction in solid tumors limits the effectiveness of cell-cycle-dependent chemotherapeutic agents. To understand the molecular basis of such resistance, we examined the expression of the cyclin-dependent kinases inhibitor p27, and relationship with drug resistance and P-gp expression in ovarian cancer multicellular spheroids. Methods: We cultured ovarian cancer cells (A2780 and CAOV3) as multicellular spheroids and examined the expression of p27 and P-glycoprotein (P-gp) by western blot, flow cytometry and confocal. We also analyzed the cell-cycle distribution by flow cytometry. In addition, trypan blue exclusion testing and cell apoptosis analysis were used to detect the sensitivity to Taxol. Results: When transferred from monolayer to three-dimensional culture, a consistent upregulation of p27 protein and P-gp protein was observed in ovarian cancer cell lines. Compared with monolayer cells, there was a significant increase of G0-G1 phase cells and decrease of S and G2-M phase cells in spheroid cells. Aggregates of cells showed higher cell viability than monolayer cells. Antisense oligodeoxynucleotide (ASON) -mediated downregulation of p27 reduced intercellular adhesion, increased cell proliferation, downregulated P-gp expression and sensitized cells to Taxol. Conclusions: Our results implicate that p27 serves as a regulator of drug resistance in ovarian tumors. ASON-mediated alteration of p27 reverses resistance of ovarian cancer to anticancer agents that are associated with increased sensitivity of ovarian cancer cells to chemotherapeutic agents.Hui Xing and Shixuan Wang have contributed equally in this work     

肺部CT灌注成像与MRI增强扫描在重度烧伤吸入性损伤中的诊断价值比较

目的通过对比分析重度烧伤吸入性损伤患者肺部CT灌注成像与MRI增强扫描结果及其应用价值,为重度烧伤吸入性损伤的临床诊断提供参考。方法回顾性分析2016年9月至2018年9月许昌市中心医院收治的70例重度烧伤吸入性损伤患者的病历资料,并按照诊断方法的不同将其分为观察组与对照组,每组35例,其中观察组患者采用肺部CT灌注成像进行检查,对照组患者采用MRI增强扫描进行检查,根据综合病史体征、临床症状及辅助检查结果进行的确诊结果对比分析两组患者的影像学诊断结果及诊断准确性。结果确诊结果显示,观察组患者中双侧胸腔积液12例、间质性肺疾病9例、肺部感染14例,对照组患者中双侧胸腔积液11例、间质性肺疾病11例、肺部感染13例,两组对比,χ^2=0.281,P=0.869,P>0.05,差异无统计学意义影像学检查结果显示,观察组患者中双侧胸腔积液12例、间质性肺疾病9例、肺部感染14例,诊断准确率为100%;对照组患者中双侧胸腔积液9例、间质性肺疾病10例、肺部感染11例,诊断准确率为85.71%,两组对比,χ^2=5.385,P=0.020,P<0.05,差异具有统计学意义。结论与肺部MRI增强扫描相比,肺部CT灌注成像对重度烧伤吸入性损伤患者的诊断更加准确,临床价值更高,有利于降低临床漏诊、误诊事件的发生率。     

A physical approach for the estimation of the SERS enhancement factor through the enrichment and separation of target molecules using magnetic adsorbents

The controllable synthesis of nanosized Fe₃O₄ (10–20 nm) encapsulated in different numbers of graphene layers (1–5 layers) (Fe₃O₄@DGL NPs) was realized through a facile and green hydrothermal reaction at a temperature as low as 200 °C. The competitive reduction–oxidation between reducing ethylene glycol (EG) and oxidizing H₂O under hydrothermal conditions resulted in the emergence of a magnetic Fe₃O₄ core. Then, the pyrolytic reaction of the polyvinyl alcohol (PVA) molecules attached to the surface of the Fe₃O₄ core with different surface densities led to the formation of graphene with a controlled number of layers. These Fe₃O₄@DGL NPs exhibited fast adsorption and sensitive SERS detection for rhodamine B (RhB). A physical and mathematical model was proposed for the estimation of the enhancement factor (EF) by combining the adsorption efficiency and SERS of RhB. This approach and model are applicable for the adsorption, sensitive SERS detection and determination of SERS EF when using functional magnetic nanoparticles as the adsorbent. The Fe₃O₄@1G NPs were also used as a novel nano-adsorbent for the fast removal of Escherichia coli (E. coli) from an aqueous solution. The Fe₃O₄@1G NPs regenerated after 3 cycles also showed high efficiency in the adsorption and separation of RhB and E. coli.

The controllable synthesis of nanosized Fe₃O₄ (10–20 nm) encapsulated in different numbers of graphene layers (1–5 layers) (Fe₃O₄@DGL NPs) was realized through a facile and green hydrothermal reaction at a temperature as low as 200 °C.     

七氟醚吸入全麻对高龄手术患者术后认知功能的影响

目的 研究七氟醚吸入全麻对高龄手术患者术后认知功能的影响.方法 选择2013年1月～2014年6月普外科老年手术患者236例,采用隐匿数字随机法分为两组,分别采用七氟醚吸入全麻和丙泊酚静脉全麻,比较两组患者术后认知功能.结果 两组术后睁眼时间、拔管时间、应答时间比较差异无统计学意义（P＞0.05）.七氟醚组术后1h、6 h MMSE评分低于丙泊酚组（P＜0.01）,24h后逐渐恢复,72 h后基本恢复至术前水平（P＞0.05）.两组术后1h均发生术后认知功能障碍（POCD）,七氟醚组术后6h POCD发生率为58.47％,明显高于丙泊酚组的29.66％（P＜0.01）.术后24h、72h POCD发生率比较差异无统计学意义（P＞0.05）.结论 七氟醚吸入全麻对高龄患者术后认知功能影响有限,72 h后可基本恢复至术前水平.建议术前积极消除患者认知功能障碍的影响因素,以提高高龄患者手术麻醉的安全性.