The relationship between the down-regulation of DNA-PKcs or Ku70 and the chemosensitization in human cervical carcinoma cell line HeLa

The aim of this study was to clarify the function of non-homologous end-joining (NHEJ) in tumorigenesis and chemoresistance, and to explore the potential of DNA-PK as a target of reversal of chemoresistance and enhancing the sensitivity of cells to chemotherapeutic agents. Plasmid vectors pSIREN-Ku70shRNA and pSIREN-DNA-PKcssh-RNA, which coded small interfering RNA of Ku70 and DNA-PKcs, were constructed and transfected into human cervical cancer cell line HeLa. The relationship between the down-regulation of Ku70 or DNA-PKcs and tumor cell proliferation and the sensitivity of cells to chemotherapeutic agents were analyzed. Down-regulation of Ku70 and DNA-PKcs expression inhibited cell proliferation, and increased cell apoptosis in DDP-treated HeLa cells. DNA-PK might play an important role in drug resistance, and inhibition of the DNA-PK expression suppressed the growth of tumor cells and enhanced the sensitivity of cells to chemotherapeutic agents.     

Computer implementation in the reconstruction of 2-D flow velocity fields in ultrasound Doppler color imaging

Doppler color imaging can easily render flow information within the vessels and simultaneously provide anatomic information for diagnostic purposes. However, the angle dependence problem of the Doppler velocity measurement is a significant barrier for continuing progress toward quantitative clinical applications of this technology. This paper presents a method and the computer implementation for reconstruction of the 2-D flow velocity field (angle independent) in ultrasound Doppler color imaging. Formulae for deriving angle independent velocity amplitude and angle direction from the color images acquired with a linear array transducer are given. The hardware configuration of the data acquiring and processing system is described. Major considerations in the development of algorithms, especially the strategies for reducing the computation time are presented.     

氟比洛芬酯用于泌尿外科腔镜手术术后镇痛的效果观察

目的：观察氟比洛芬酯用于泌尿外科腔镜手术术后镇痛的有效性和安全性,比较氟比洛芬酯超前镇痛和氟比洛芬酯常规镇痛的效果差异。方法：选择ASAI-II级择期行泌尿外科腔镜手术的患者120例,随机分为氟比洛芬酯超前镇痛组（A组）、氟比洛芬酯常规镇痛组（B组）、安慰剂组（C组）,均实施腰-硬联合麻醉。A组术前15min静推氟比洛芬酯注射液1mg／kg,B组手术结束前15min静推氟比洛芬酯注射液lmg／kg,C组术前15min静推10ml生理盐水。记录患者术后3、6、12、24h~,觉模拟（vas）评分、镇痛满意度、追加补救镇痛药的例数和时间及不良反应的发生例数。结果：A组术后6、12、24hVAS评分明显低于同时间点的C组．B组术后6h、12hVAS评分明显低于同时间点的C组（P〈0．05）。镇痛满意度评价A、B两组均明显优于C组（P〈0．05）。补救镇痛药物的使用例数A、B两组均明显少于C组（P〈0．05）,使用时间A组长于B、C两组、B组长于C组。三组不良反应的发生情况无差另q。结论氟比洛芬酯用于泌尿外科腔镜手术术后镇痛是安全有效的,并且氟比洛芬酯超前镇痛的效果要优于常规镇痛。     

双向聚类方法的文献计量分析

目的：拓展双向聚类方法在中医药领域数据分析中的应用,使数据分析研究人员掌握双向聚类方法的研究动态和潜在应用价值。方法：本文通过描述统计分析方法和文献同引网络分析方法等方法来研究双向聚类方法的发展趋势、热点研究领域和未来的发展方向。结果：双向聚类方法的研究热点主要集中在算法研究、生物信息学数据分析、信息检索和文本挖掘等方面。结论：结合双向聚类方法与中医药数据分析的特点,双向聚类方法能用来识别具有类似症状群的患者人群的特征,探索症状分类与患者特征的关系,探索药物配伍特点、实现精细化治疗,探索量效关系、实现动态和科学合理用药,探索组学研究与中医症候、中医治疗方案的关系等研究中。     

卵巢癌患者手术前后血清IL-2、TGF-α、TNF-α和TSGF测定的临床意义

目的：探讨卵巢癌患者手术前后血清细胞因子水平的变化及测定的意义。方法：用放射免疫分析（RIA）和生化法分别测定了32例卵巢癌及34名对照组血清IL-2、TGF-α、TNF-α及TSGF4项指标水平,并将测定结果进行了数据处理和分析探讨。结果：卵巢癌患者手术治疗前血清TGF—α、TNF—α和TSGF3项指标水平较对照组升高均极显著（P均〈0．01）,IL-2水平则较对照组降低极显著（P〈0．01）;手术治疗3个月后前述4项指标水平与对照组比较差异均无显著性（P均〉0．05）,而复发者则4项指标水平较未复发者差异均非常显著（P〈0．01,P〈0．05）。结论：IL-2、TGF-α、TNF-α和TSGF的测定对于卵巢癌的诊断、疗效观察和预后评估均有重要帮助。     

丹酚酸B对脊髓继发性损伤的保护

目的：探讨丹酚酸B对大鼠急性脊髓损伤的影响和潜在机制。 方法：参照Allen法制作SD大鼠T9脊髓节段急性损伤模型,按给予Sal B剂量的不同分组.各组用比色法检测髓过氧化物酶的活性,用免疫组织化学染色检测损伤脊髓节段基质金属蛋白酶1和c-Fos的表达情况,用干湿重法评价损伤脊髓的水肿程度,并采用BBB评分评价脊髓损伤后14天内大鼠的运动功能恢复。接着将50只急性脊髓损伤大鼠随机分为5组,分别给予Sal B和依那西普的不同处理,用RT-PCR和western blot检测大鼠脊髓损伤节段肿瘤坏死因子-α的mRNA和蛋白表达水平的变化。 结果：损伤后4小时髓过氧化物酶活性检测显示,经Sal B处理组MPO活性均下降,与对照组相比,MPO活性下降在高剂量组(20mg/kg组)最明显,统计学分析P<0.05。损伤后1天组织切片免疫组化染色结果显示,Sal B处理组比PBS对照组MMP-1表达减少,c-Fos表达下调,高剂量组(20mg/kg组)与PBS组相比有统计学意义（P<0.05）。损伤后3天Sal B治疗组水肿程度较PBS组低,高剂量组(20mg/kg组)与PBS组相比有明显差异（P<0.05）。Sal B组高剂量组(20mg/kg组)和PBS对照组之间的BBB评分有明显差异（P<0.05）。RT-PCR和Western blot均显示,Sal B+ etanercept治疗组与Sal B或etanercept单独应用组相比,其对TNF-alpha mRNA和蛋白的表达下调作用更显著（P<0.05）。 结论：大鼠急性脊髓损伤后用Sal B处理能减轻脊髓细胞损伤,促进大鼠运动功能障碍的恢复。实验提示Sal B可能通过降低MMP-1、c-Fos和肿瘤坏死因子-α的表达而起到抗氧化和抗炎作用,并且Sal B减轻脊髓损伤的作用与其剂量呈正相关性,提示丹酚酸B能减少急性脊髓损伤后相关细胞因子对组织的伤害而发挥细胞保护作用。     

A Preliminary Study on the Relationship between Serum Heparan Sulfate and Cancer-Related Cognitive Impairment: The Moderating Role of Oxidative Stress in Patients with Colorectal Cancer

Cancer-related cognitive impairment (CRCI) has been frequently reported in colorectal cancer survivors. Heparan sulfate (HS) was gradually considered to be related to cognitive disorders. The effect and potential mechanism of HS on CRCI in colorectal cancer patients were unexplored. In this study, all participants were divided into a cognitive impaired group and a cognitive normal group. The concentrations of oxidative stress factors and HS in serum were detected. Associations among HS, oxidative stress factors and CRCI were evaluated. Participants with cognitive impairment exhibited increased levels of HS, GSH, SOD and MDA, compared to the patients with normal cognitive performance. The independent significant association was found between HS and CRCI after controlling for various covariates. The higher concentrations of HS were related to the decreased cognitive performance among survivors who reported higher levels of GSH (β = 0.080, p = 0.002). Moreover, the nonlinear association between the level of HS and cognitive scores was confirmed using the restricted cubic splines (p < 0.001). These results indicated that the increased concentrations of circulating HS had a nonlinear negative connection with cognitive performance in colorectal cancer survivors, which was moderated by GSH. HS might be a new biomolecule for the identification and management of patients with CRCI.     

三磷酸腺苷结合盒转运子A7在脂质转运过程中作用的研究

目的:探讨三磷酸腺苷结合盒转运子A7(ABCA7)在细胞内脂质流出过程中的作用。方法:以apoAI刺激转染ABCA7-或ABCA1-基因的HEK293细胞24h,利用蛋白印迹法以及酶分析法分别测定ABCA7的变化与细胞内胆固醇和磷脂的流出。结果:apoAI分别上调ABCA7或ABCA1蛋白量,ABCA7与ABCA1同样促进细胞内脂质的转运,其中磷脂的流出较胆固醇流出更为明显。结论:ABCA7与ABCA1同样具有促进细胞内脂质转运的功能。     

真核表达Survivin基因对卵巢癌细胞紫杉醇敏感性的影响

目的探讨Survivin基因对卵巢癌细胞紫杉醇（Taxol）敏感性的影响。方法构建Survivin正义全长真核表达质粒（pcDNA3．1-Survivin），经脂质体包裹转染人卵巢癌细胞A2780，以转染pcDNA3．1空载体的A2780细胞为对照。RT—PCR和蛋白质免疫印迹（Western blot）法检测Survivin mRNA和蛋白质的表达；MTT比色法检测紫杉醇对两组细胞存活率的影响；流式细胞仪（FACS）检测细胞凋亡率。结果①RT—PCR和Western blot检测提示转染pcDNA3．1-Survivin组中Survivin mRNA和蛋白质表达明显高于空载体组。②MTT比色法和FACS检测提示转染pcDNA3．1-Survivin组细胞存活率明显高于空载体组，细胞凋亡率明显低于空载体组，差异均有显著性意义（P〈0．05）。pcDNA3．1-Survivin转染后的A2780细胞对紫杉醇的敏感性明显降低。结论卵巢癌对紫杉醇的耐药性可能与Survivin基因表达有关。     

Adjuvant adenovirus-mediated delivery of herpes simplex virus thymidine kinase administration improves outcome of liver transplantation in patients with advanced hepatocellular carcinoma.

PURPOSE: Previous poor results of liver transplantation (LT) have been confirmed in patients with advanced hepatocellular carcinoma (HCC). Adenovirus-mediated delivery of herpes simplex virus thymidine kinase (ADV-TK) therapy is an established adjuvant treatment in cancer, and we evaluated its potential as an adjuvant treatment for HCC patients who underwent LT. EXPERIMENTAL DESIGN: Forty-five HCC patients with tumors >5 cm in diameter participated in the study over a follow-up period of 50 months. Among these patients, 22 received LT only, and 23 received LT combined with ADV-TK therapy. All HCC patients enrolled in this study had tumors >5 cm in diameter and no metastasis in lungs or bones was detected by computed tomography or magnetic resonance imaging scans. RESULTS: The recurrence-free survival and the overall survival in the LT plus ADV-TK therapy group were 43.5% and 69.6%, respectively, at 3 years; both values were significantly higher than those in the LT-only group (9.1% and 19.9%, respectively). In the nonvascular invasion subgroup, overall survival was 100% and recurrence-free survival was 83.3% in the patients receiving LT plus ADV-TK, significantly higher than the patients receiving LT only. CONCLUSIONS: HCC patients with no vascular invasion could be selected for LT followed by adjuvant ADV-TK therapy, regardless of intrahepatic huge or diffuse tumor. We propose that the current criteria for LT based on tumor size may be expanded if accompanied by ADV-TK therapy due to improved prognosis.