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991.
膜蛋白降解对红细胞膜脂流动性的影响 总被引:1,自引:0,他引:1
目的与方法:本文通过实验的方法,采用胰蛋白酶降解红细胞膜蛋白,研究了膜蛋白降解对红细胞膜脂流动性的影响。结果:随着胰蛋白酶作用浓度的升高,经马来酰亚胺(MSL)标记的膜蛋白ESR谱表现为弱强固定比(S/W)趋于上升,DPH标记的膜脂荧光偏振度(P)值上升,且呈现出明显的量效关系;结论;这意味着由于胰蛋白酶的作用,降解了红细胞膜蛋白,使得膜蛋白结构趋于收缩,最终导致了膜脂流动性的降低。 相似文献
992.
用Bolton-Hunter试剂联结法标记胆囊收缩素(CCK8),得125I-BH-CCK8,其比放射性为3.4TBq/mmol,放射化学纯度大于96%。取其与自制的大鼠大脑皮质细胞膜进行受体放射分析,发现标记配体与大鼠大脑皮质CCK受体的结合具有温度和时间依赖性,可饱和性,可逆性及特异性。经Scatchard分析获大鼠大脑皮质细胞膜CCK受体Kd值为1.098nmol/L,Bmax为197.5fmol/mg蛋白。 相似文献
993.
BCL-2、p53蛋白在胆管癌组织中的表达 总被引:2,自引:0,他引:2
目的 研究BCL-2蛋白、P53蛋白表达与胆管癌预后的关系。方法 应用免疫组化研究40例胆管癌组织中BCL-2蛋白、p53蛋白表达及其与胆管癌组织类型及术后生存期的关系。结果 胆管癌组织中BC 白和P53蛋白表达旨也生率分别为55%和47.5%;BCL-2蛋白与P53蛋白的表达呈负相关,低分化癌P53蛋白阳性率高于高分化癌,高分化癌BCL-2蛋白阳性率高于低分化癌;BCL-2蛋白表达高或P53蛋白 相似文献
994.
谷胱甘肽转移酶π在胃癌的表达及与预后的关系 总被引:2,自引:0,他引:2
目的 探讨光甘肽转移酶π(GST-π)与胃癌的分型、分期及预后的关系。方法 应用鼠抗人GST-π单克隆抗体对80例胃癌进行免疫组化研究。结果 GST-π在癌旁正常组织中的是性表达与生存期有统计学意义(P〈0.05);在癌组织中的表达强度与平均生存期有关(P〈0.05)。GST-π表达与临床分期及胃癌组织分化程度无关(P〉0.05)。不同生存年段中GST-π的表达也无统计学差异(P〉0.05)。结论 相似文献
995.
目的探讨重度颅脑损伤病人康复期智力、记忆力及情感等方面的改变。②方法根据格拉斯哥昏迷指数(GCS)所规定的等级,对41例重度颅脑损伤病人进行智力、记忆力能力测验,并与我国常模比较。③结果康复优良组病人的操作智商、语言商数均在正常范围。重残组75.0%的病人语言商数明显降低,36.8%轻残组病人和100%重残病人操作智商降低。④结论严重颅脑损伤病人存在着智力、记忆力和情感障碍,重度残疾病人以操作智商降低为主,康复优良病人常出现智力与记忆力缺陷。 相似文献
996.
选择湖北麻城市属5个乡84个村为调查单位,共调查1992~1994年女性自杀死亡病例199例(调查率9170%).经对获取资料进行的流行病学分析,结果提示:1992~1994年麻城妇女自杀死亡率分别是6449/10万、85.00/10万和78.14/10万;其中,20~29岁,30~39岁以及60~69岁为死亡人数高峰组;自杀方式以服毒者居多。 相似文献
997.
Shi XQ Eklund I Tronje G Welander U Stamatakis HC Engström PE Engström GN 《Dento maxillo facial radiology》1999,28(1):31-36
OBJECTIVES: To assess intra- and interobserver agreement on marginal changes in periodontal bone from color-coded compared with subtraction radiographs. METHODS: Sequential radiographs from patients undergoing periodontal treatment were acquired using direct digital intra-oral radiography. Fifty-one pairs of color-coded and subtraction radiographs were produced and evaluated twice by six dentists for changes in marginal bone. Intra- and interobserver agreement were calculated. RESULTS: Intra-observer agreement was significantly higher for the color-coded radiographs (P < 0.05). Interobserver agreement was significantly higher for color-coded radiographs at the second (P < 0.001) but not the first (P = 0.34) evaluation. CONCLUSIONS: Color coding of radiographic differences by means of image addition may be a feasible alternative to the subtraction technique for evaluating periodontal bone changes. 相似文献
998.
Free fatty acids impair hepatic insulin extraction in vivo 总被引:11,自引:0,他引:11
Wiesenthal SR Sandhu H McCall RH Tchipashvili V Yoshii H Polonsky K Shi ZQ Lewis GF Mari A Giacca A 《Diabetes》1999,48(4):766-774
Hyperinsulinemia is a common finding in obesity and results from insulin hypersecretion and impaired hepatic insulin extraction. In vitro studies have shown that free fatty acids (FFAs), which are often elevated in obesity, can impair insulin binding and degradation in isolated rat hepatocytes. To investigate whether FFAs impair hepatic insulin extraction (E(H)) in vivo, either saline (SAL) or 10% Intralipid (0.03 ml x kg(-1) x min(-1)) plus heparin (0.44 U x kg(-1) x min(-1)) (IH) was infused into normal dogs to elevate FFA levels. Insulin was infused intraportally at 18 pmol x kg(-1) x min(-1) for 150 min (period A, high insulin dose), and then at 2.4 pmol x kg(-1) x min(-1) for another 150 min (period B, low insulin dose). After the low portal insulin dose, additional insulin was infused peripherally at 8.4 pmol x kg(-1) x min(-1) for 120 min (period C) to assess the clearance of insulin from the peripheral plasma. In 16 paired experiments, FFA levels were 1,085 +/- 167, 1,491 +/- 240, 1,159 +/- 221 micromol/l (IH) and 221 +/- 44, 329 +/- 72, 176 +/- 44 micromol/l (SAL) in periods A, B, and C, respectively. Peripheral insulin levels were greater with IH (P < 0.001) than with SAL in all periods (1,620 +/- 114, 126 +/- 12, 1,050 +/- 72 pmol/l for IH vs. 1,344 +/- 168, 96 +/- 4.2, 882 +/- 60 pmol/l for SAL). Glucose clearance was impaired by IH in all periods (P < 0.05), whereas glucose production was slightly increased by IH during period B. Peripheral insulin clearance (Cl) and E(H) were calculated from the insulin infusion rate and insulin concentration data in each period by taking into account the nonlinearity of insulin kinetics. Cl was lower (P < 0.01) with IH (9.6 +/- 0.6, 12.0 +/- 0.9, 10.2 +/- 0.6 ml x kg(-1) x min(-1)) than with SAL (11.2 +/- 1, 13.6 +/- 0.7, 11.9 +/- 0.9 ml x kg(-1) x min(-1)) in periods A, B, and C. E(H) was also lower (P < 0.05) with IH (25 +/- 4, 40 +/- 5, 32 +/- 5%) than with SAL (30 +/- 2.8, 47 +/- 3, 38 +/- 3%). We conclude that FFAs can impair hepatic insulin extraction in vivo at high and low insulin levels, an effect that may contribute to the peripheral hyperinsulinemia of obesity. 相似文献
999.
1000.
Assadi FK McCue P Jefferis S Shi M Beckman DA 《Pediatric nephrology (Berlin, Germany)》1999,13(9):812-815
The safety of cysteamine after renal transplantation and during pregnancy is an important issue, since girls with cystinosis
are in better health on cysteamine therapy and thus more likely to become pregnant. In the first study, cysteamine was given
to pregnant rats on days 6.5–18.5 post conception in oral doses of 0, 37.5, 75, 100, and 150 mg/kg per day. The dams were
sacrificed on day 20.5, the fetal kidneys removed and prepared for histological examination. In the second study, cysteamine
was given to dams on days 6.5–19.5 post conception in oral doses of 0, 37.5, 50, and 75 mg/kg per day. Dams were allowed to
give birth naturally and pups were given cysteamine on days 4–21 to yield the same oral doses of cysteamine given to the dam.
Renal function was evaluated on day 35. Histological examination of fetal kidneys revealed no changes even in kidneys from
fetuses with growth retardation and malformations. Furthermore, there were no alterations in renal function in offspring on
day 35. These findings demonstrate that cysteamine therapy does not affect renal development in the rat. Further investigations
will be required to prove whether cysteamine therapy has the potential to affect renal development in the human.
Received: 25 September 1998 / Revised: 17 November 1998 / Accepted: 13 December 1998 相似文献