多参数流式细胞术在多发性骨髓瘤诊断中的价值

目的:探讨多发性骨髓瘤(MM)患者免疫表型表达特点。方法:应用多参数流式细胞术(MP-FCM)测定30例MM患者和8例继发性单克隆免疫球蛋白血症骨髓细胞免疫表型。结果:骨髓瘤细胞CD138和CD38均阳性,86.7%CD56阳性,而CD45阴性或弱阳性,93.3%CD19阴性;继发性单克隆免疫球蛋白血症患者CD19、CD38、CD138、CD45均阳性,CD56阳性率12.5%。结论:应用MP-FCM同时检测细胞表面CD38、CD138、CD19、CD56、CD45表达情况,可方便区分骨髓瘤细胞与良性浆细胞,是MM的重要诊断依据。     

血管内皮生长因子受体在骨髓增生异常综合征中的作用研究

目的:探讨血管内皮生长因子受体(VEGFR)在骨髓增生异常综合征(MDS)发病机制中的作用。方法:用流式细胞仪测定MDS患者及正常人骨髓单个核细胞VEGFR(VEGFR1和VEGFR2)表达率。结果:VEGFR1阳性表达率结果显示:MDS高危组>MDS低危组>正常组(P<0.01);MDS组和正常组的VEGFR2表达结果无差异(P>0.05)。结论:MDS患者VEGFR1阳性表达增高,VEGFR1阳性表达增高与MDS病程进展有关,提示在MDS转化为白血病过程中VEGFR1起重要作用。     

螺旋CT血管造影在动脉瘤诊断中的临床应用和价值

[目的]探讨螺旋CT血管造影在动脉瘤诊断中的临床应用和价值。[方法]21例经手术证实的动脉瘤患者进行了SCTA检查,成像技术为遮盖法(SSD)和最大强度法(MIP),其中7例同时做了血管造影检查。[结果]21例动脉瘤患者术前均准确定性、定位、与手术结果完全一致。[结论]SCTA作为一种无创性血管成像方法,在诊断动脉瘤方面,具有分辨率高,快速、准确、经济等优点,SSD较MIP能提供更多的信息,基本取代了传统的血管造影。     

毓明方对治疗性视网膜激光损伤的防治作用

目的：观察中药毓明方对治疗性视网膜激光损伤的防治作用。方法：采用自身前后对照设计。接受激光治疗的２７例患者随机分为空白对照组、西药组和中药组。同等状况的激光光凝治疗后２４小时开始用药。１个月后测定激光区视野平均视敏度阈值,并与治疗前比较。结果：药物治疗后中药组阈值与其他两组比较明显改善（Ｐ＜００５,Ｐ＜００１）。结论：毓明方可改善激光光凝治疗区视野视敏度的平均视阈值,对治疗性激光视网膜损伤有防治作用。     

Increased expression of xanthine oxidase and insulin-like growth factor I (IGF-I) immunoreactivity in skeletal muscle after strenuous exercise in humans

The present study investigated the effect of 7 days of strenuous exercise on the quantity of xanthine oxidase and IGF-I in muscle. Fifteen male military trainees performed 1 week of terrain marching and warfare exercises. Muscle biopsies and blood samples were obtained prior to and after the week. After the week, the number of xanthine oxidase immunoreactive cells, identified as capillary endothelial cells and leucocytes, and the number of IGF-I immunoreactive cells, mainly vascular cells but also cells tentatively identified as satellite cells, were higher in the muscle (P<0.05). Plasma creatine kinase activity was 650% higher after the week (P<0.001) and the muscle content of hydroxyproline was elevated by 160% 2 months post-exercise (P< 0.05), both findings implying injury to the muscle. The present data provide a first observation of an elevated level of xanthine oxidase and IGF-I in human skeletal muscle after exercise. It is proposed that both substances increased as a result of cellular damage: xanthine oxidase because of the influence of immunomodulators, and IGF-I in association with regenerative processes. The increased expression of IGF-I in the muscle could, however, also reflect cellular growth in response to an elevated load on the muscle and the vascular bed.     

截瘫患者并发感染及其危险因素分析

目的分析截瘫患者发生感染的危险因素与感染的关系.方法应用成组病例对照研究方法,将危险因素与并发感染的关系进行单因素和多因素分析,筛选各因素与感染的关系.结果 302例住院截瘫患者并发感染139例,感染率46.0%,医院感染率27.8%;住院时间、截瘫程度、并发糖尿病、血浆白蛋白浓度等因素与并发感染差异有显著性(P<0.05).结论并发感染与截瘫程度和住院时间呈正相关,与血浆白蛋白浓度呈负相关.     

上海市区大气中真菌污染状况的研究

[目的 ]　调查上海市区大气中真菌污染状况及对策研究。 [方法 ]　采用空气自然沉降法于 1993年 7月～ 1994年 4月调查上海市区大气真菌污染状况。 [结果 ]　单细胞真菌功能区年平均数 7.2 4个 /皿 ,明显低于特殊区年平均数 2 7.30个 /皿 ,功能区单细胞真菌污染以交通、商业区较高 ,居住工业混合区、清洁区较低。多细胞真菌地区分布在不同季节不一致。真菌分布有明显季节性规律 ,单细胞真菌以冬春季为高峰 ,夏秋季降低 ,而多细胞真菌则夏秋季升高 ,冬春季较低。除夏季外 ,大气中单细胞、多细胞真菌数均呈日高夜低的日周期变化规律 ,前者以 10～ 14时为高峰 ,后者高峰随季节变化较大。真菌的空间分布一般以 1.2米处高于 15米处。 [结论 ]　上海市区大气真菌污染有一定地区分布 ,季节性分布规律。单细胞和多细胞真菌分布规律不同。     

A complete sequence of the T. tengcongensis genome

Thermoanaerobacter tengcongensis is a rod-shaped, gram-negative, anaerobic eubacterium that was isolated from a freshwater hot spring in Tengchong, China. Using a whole-genome-shotgun method, we sequenced its 2,689,445-bp genome from an isolate, MB4(T) (Genbank accession no. AE008691). The genome encodes 2588 predicted coding sequences (CDS). Among them, 1764 (68.2%) are classified according to homology to other documented proteins, and the rest, 824 CDS (31.8%), are functionally unknown. One of the interesting features of the T. tengcongensis genome is that 86.7% of its genes are encoded on the leading strand of DNA replication. Based on protein sequence similarity, the T. tengcongensis genome is most similar to that of Bacillus halodurans, a mesophilic eubacterium, among all fully sequenced prokaryotic genomes up to date. Computational analysis on genes involved in basic metabolic pathways supports the experimental discovery that T. tengcongensis metabolizes sugars as principal energy and carbon source and utilizes thiosulfate and element sulfur, but not sulfate, as electron acceptors. T. tengcongensis, as a gram-negative rod by empirical definitions (such as staining), shares many genes that are characteristics of gram-positive bacteria whereas it is missing molecular components unique to gram-negative bacteria. A strong correlation between the G + C content of tDNA and rDNA genes and the optimal growth temperature is found among the sequenced thermophiles. It is concluded that thermophiles are a biologically and phylogenetically divergent group of prokaryotes that have converged to sustain extreme environmental conditions over evolutionary timescale.