奥硝唑用于口腔厌氧菌感染的临床研究

目的　评价奥硝唑治疗口腔厌氧菌感染疾病的效果。方法　选择口腔厌氧菌感染疾病患者 60例。以替硝唑为对照组 ,采用双盲法 ,对其临床疗效和细菌清除率进行比较。结果　奥硝唑总有效率 83 .3 % ,细菌清除率 91.7% ;替硝唑总有效率 73 .3 % ,细菌清除率 88.4% ,二者间无显著性差异 (P >0 .0 5 )。结论　奥硝唑治疗口腔厌氧菌感染疾病效果显著。     

结核性心脏病变的实验研究──豚鼠急性结核感染实验模型

本文用结核菌感染动物模型探讨结核菌对心脏组织感染及病理损害程度。以Ｈ３７Ｒｖ为感染菌株，豚鼠为感染动物，感染途径经腹腔注射。结果表明，毒力指数为８０的Ｈ３７Ｒｖ菌、以０．２ｍｌ（１×１０７／ｍｌ）的菌量感染豚鼠，其脏器感染软弱。尽管如此，肝、脾、肺、肾、琳巴结等组织出现了典型的结核性病理改变；并有５０％以上的心脏组织（心包膜、心肌、主动脉干）也出现了病理改变。心包膜以单核细胞、粒细胞和纤维细胞增生为主；心肌的病理改变为局灶型、以肌纤维断裂、溶解、并伴单核细胞和淋巴细胞聚集为特征；主动脉干的内缘增厚，并伴粒细胞和单核细胞浸润。因此，在临床上必须考虑是否存在结核菌对心脏的损害，并采取相应的治疗措施。     

L-精氨酸对高脂血症家兔血浆脂蛋白、一氧化氮和脂过氧化物的影响

用雄性新西兰白兔观察了Ｌ－精氨酸对高脂血症时血浆脂蛋白、血清一氧化氨、脂过氧化物和超氧化物歧化酶的影响。结果发现２％Ｌ－精氨酸与２％胆固醇同时喂养９０天，或喂养９０天以后４％Ｌ－精氨酸治疗９０天，均能有效地抑制高胆固醇所致的血清脂过氧化物的升高，提高高脂血症家兔血清超氧化物歧化酶活性，促进血管内皮细胞一氧化氮的释放。结果提示，Ｌ－精氨酸／一氧化氮可能有抗高脂血症家兔低密度脂蛋白氧化修饰的作用。     

抗hnRNPA2/B1多克隆抗体制备及其在非小细胞肺癌中的应用

ObjectiveIn order to evaluate potential application for diagnosis and prognosis of non-small celt lung cancer (NSCLC),as well as to determine its role in the pathogenesis of the disease,we prepared anti-human hnRNP A2/B1 polyclonal antibody.MethodsProkaryotic expression vector of pET28a ( )-hnRNP A2/B1 was constructed and transformed into E.coli BL21.The recombinant protein induced by IPTG was purified and injected to rabbits for antibody preparation.Expression of hnRNP A2/B 1 was examined in 45 tissues of NSCLC and 16 inflammatory pseudotumor tissues of lung by immunohistochemistry with the antibody.The commercial hnRNPA2/B1 monoclonal antibody was used as a control.Results(t) Polyclonal antibody against hnRNP A2/B1 with high title was obtained.(2) The positive staining in NSCLC tissues was 62.22%,which was substantially higher than that in normal tissues (40%,P＝0.035) or inflammatory pseudotumor tissues (31.25%,P＝0.033).(3) Expression of hnRNP A2/B1 positively correlated with age and the history of smoking,whereas it negatively correlated with differentiation staging of tumors.(4) Follow-up study showed that the survival time of patients with positive staining was significantly shorter than that of patients without hnRNP A2/B1 expression (P＝0.048).ConclusionIt is successful to make the recombinant protein and prepare the polyclonal antibody agonist human hnRNP A2/B1.It may be a valuable marker for the diagnosis and prognosis of NSCLC.Our results provide a basis for further study in clinical application.     

血管内皮生长因子及其受体在子宫肌瘤发病中作用的研究

目的 探讨血管内皮生长因子(VEGF)及其受体(VEGFR1和VEGFR2)在子宫肌瘤发病中的作用.方法 研究对象为2005年6月至9月期间在上海交通大学医学院附属仁济医院住院接受子宫切除术治疗的子宫肌瘤患者30例.超声测量患者子宫肌瘤数量及其径线值.术中取小块子宫肌瘤及正常肌层组织,采用免疫印迹法(Western blot)测定其中VEGF及其受体蛋白含量.结果 子宫肌瘤组织中VEGF及VEGFR1、VEGFR2蛋白表达水平(0.74±0.30、0.44±0.13、0.75±0.40)均显著高于正常肌层组织(0.15±0.07、0.36±0.14、0.31±0.21,P<0.01);无论是增生期或分泌期,它们在子宫肌瘤组织中均持续高表达(P<0.01),但各自月经周期差异无统计学意义(P>0.05);VEGFR2表达水平显著高于VEGFR1(P<0.01);VEGF和VEGFR2表达水平与子宫肌瘤数量呈正相关(P<0.05).结论 VEGF及其受体与子宫肌瘤的发病密切相关;如能研制VEGF及其受体蛋白的拮抗剂,则可能导致子宫肌瘤萎缩或消亡,可能是子宫肌瘤药物治疗的一个新途径.     

The importance of the coal tar bath in the Ingram treatment of psoriasis. Evaluation by evaporimetry and laser Doppler flowmetry

The contribution of the tar component to the efficacy of the Ingram regimen in the treatment of psoriasis was assessed in 11 patients with symmetrical lesions on the upper extremities. One arm was immersed in an oil emulsion bath and the other in a coal tar bath. Whole body ultraviolet irradiation followed, and dithranol paste was applied to all lesions. Healing was assessed clinically at weekly intervals, and was found to parallel the normalization of transepidermal water loss (TEWL) as determined by evaporimetry, and the dermal blood flow as evaluated by laser Doppler flowmetry. Results obtained with coal tar baths were not significantly different from those obtained with oil emulsion. We conclude that coal tar bath additive has no advantage over oil emulsion in the Ingram regimen.     

Protection of auditory function against noise trauma with local caroverine administration in guinea pigs

Glutamate is the most likely neurotransmitter at the synapse between the inner hair cell and its afferent neuron in the peripheral auditory system. Intense noise exposure may result in excessive glutamate release, binding to the post-synaptic receptors and leading to neuronal degeneration and hearing impairment. The present study investigated the protective effect of caroverine, an antagonist of two glutamate receptors, N-methyl-D-aspartate and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, on noise-induced hearing loss. Two different doses of caroverine were applied onto the round window membrane with gelfoam, followed by one-third-octave band noise centered at 6.3 kHz (110 dB SPL) for 1 h. Auditory brainstem responses were measured at regular time intervals afterwards. Caroverine was found to offer significant protection of the cochlear function against noise-induced hearing loss.     

老年缺血性脑血管病血瘀证患者的红细胞变形性及一氧化氮水平的检测及其意义

目的 :探讨老年缺血性脑血管病血瘀证患者的红细胞变形性及一氧化氮 ( NO)水平的变化。方法 :检测 5 5例老年缺血性脑血管病血瘀证患者的红细胞变形指数和 NO的水平。其中短暂性脑缺血发作 ( TIA) 2 5例 ,脑梗死 3 0例 ;另设 2 6例正常对照组作对比观察。结果 :血瘀证 TIA患者红细胞变形指数 ( 0 .4 67± 0 .14 5 )显著低于正常对照组 ( 0 .5 0 8± 0 .14 1) ,P<0 .0 5 ,脑梗死患者红细胞变形指数 ( 0 .4 43± 0 .15 6)降低更为明显( P<0 .0 1) ;NO水平 ,TIA患者与正常对照组比较无显著性差异 ( 79.10± 15 .3 7比 76.70± 17.10 ) ,而脑梗死患者 ( 88.5 0± 13 .68)显著升高。结论 :红细胞变形性改变及 NO水平升高在一定程度上均参与了老年缺血性脑血管病血瘀证的发生发展     

地尔硫治疗原发性高血压27例

本文用随机双盲组间对照的方法,对53例原发性高血压进行治疗对比.地尔硫组27例(男20例,女7例;年龄50±SD7yr;病程14±7yr),用最大剂量270mg/d,4wk为一疗程。维拉帕米组26例(男25例,女1例;年龄52±6yr病程14±8yr)用最大剂量360mg/d,4wk为一疗程。总有效率前者大于后者(85％,46％)但差异不显著,降压幅度亦是前者大(2.0/2.0kPa,1.0/1.0kPa,P<0.01,0.05)2药均有升高HDL-ch的作用,副作用均较轻.     

幕上胶质瘤术后近期预后的相关因素分析

目的：分析影响颅内幕上胶质瘤手术近期预后的相关因素。方法：共86例颅内幕上胶质瘤按入院时有无精神症状、癫痫、神经系统定位症状、肿瘤有无囊变、是否有CT强化及强化性质分成两组。随访术后6个月Karmofsky评分,并以单因素方差分析进行统计学分析。结果：按年龄分析,30~39岁组近期预后最佳;术前存在癫痫者预后优于无癫痫,而精神症状者相反;术前无神经系统定位症状及全身情况佳者近期预后也佳;CT扫描显示等密度或高密度、能均匀强化者预后最好（P值＆lt;0.05）。结论：患者年龄、术前全身情况、临床症状和体征以及CT扫描情况显著影响颅内幕上胶质瘤预后。