Contribution of active Na+ and Cl- fluxes to net ion transport by alveolar epithelium

Changes in bioelectric properties of alveolar epithelial cell monolayers due to pharmacological agents such as beta-agonists, amiloride and ouabain have recently been reported. In order to determine specifically which ionic species contribute to these changes, fluxes of Na+ and Cl- across primary cultured monolayers of rat type II pneumocytes were directly measured. Monolayers were mounted in modified flux chambers and short-circuited. Unidirectional fluxes of 22Na (or 36Cl) and [14C]-mannitol were measured simultaneously. Experimental maneuvers included apical (A) exposure to 10 microM amiloride, basolateral (B) exposure to 1 mM ouabain, or basolateral exposure to 20 microM terbutaline. Results show that baseline monolayers actively reabsorb Na+ (about 0.14 micro Eq.cm-2.h-1) from the apical fluid, while mannitol and Cl- appear to traverse the alveolar epithelium passively. Active Na+ reabsorption was abolished by amiloride or ouabain, while Cl- and mannitol fluxes were unaffected. Terbutaline, on the other hand, markedly increased net absorption of Na+ and caused active transport of Cl- in the A to B direction. Passive mannitol flow was somewhat increased with terbutaline. These data indicate that active Na+ reabsorption across alveolar epithelial monolayers is dependent on intact Na+,K(+)-ATPase activity and cell Na+ entry (probably via Na+ channels), and can be stimulated by beta-agonists. Beta-agonists also cause active reabsorption of Cl- (passive under other conditions).     

Health status and intimate partner violence: a cross-sectional study.

STUDY OBJECTIVE: We identify health variables associated with a history of intimate partner violence (IPV) using self-reported and laboratory measures. METHODS: This study used a cross-sectional design. Participants were a randomized sample of English-speaking women between the ages of 18 and 50 years who presented to a large urban emergency department. Potential participants were screened in the ED for a history of physical abuse and coded as having experienced no IPV (No IPV), as having a recent history of IPV (occurring in the previous 12 months; IPVA), or as having a remote history (most recent occurrence >12 months ago; IPVHx). Participants were interviewed several days later in an outpatient setting regarding demographics, medical care use, and physical and mental health variables. Participants also received urine and blood tests and a pelvic examination. RESULTS: Self-reported health was poorest among women reporting IPVA and best among women reporting no IPVA. Women in the IPVA group differed from women with no IPV history with respect to cocaine use (odds ratio [OR] 4.8; 95% confidence interval [CI] 1.4 to 17.3), sexually transmitted diseases (OR 5.1; 95% CI 1.5 to 20.3), and nightmare frequency (OR 11.6; 95% CI 2.3 to 83.4). Women reporting IPVHx were more likely to report a history of sexually transmitted diseases than women with no IPV history (OR 4.1; 95% CI 1.6 to 11.4) and had more frequent nightmares (OR 5.0; 95% CI 1.3 to 24.9). Urine and blood tests identified only 2 variables (hemoglobin levels, mean corpuscular volume) that differed significantly between groups by IPV history; these differences were not clinically significant. CONCLUSION: Women with a recent history of IPV reported a poorer health status than women with no IPV history; laboratory testing detected few differences.     

Respiratory Sensitization of a Food Manufacturing Worker to Konjac Glucomannan

Intake of paracetamol has been associated with development of asthma. The aim of this study was to address a possible association between intake of paracetamol and risk of adult-onset asthma. Using a multidisciplinary postal questionnaire survey concerning health and lifestyle we prospectively studied 19,349 adult twins enrolled in the nationwide Danish Twin Registry. There was a higher prevalence of new-onset asthma in subjects who reported frequent intake of paracetamol at baseline compared with subjects without this determinant (12.0% vs. 4.3%), OR = 3.03 (1.51–6.11), p = 0.005. The result remained significant after adjusting for sex, age, smoking, BMI, hay fever, eczema, and intake of medications other than paracetamol, OR = 2.16 (1.03–4.53), p = 0.041. Frequent intake of paracetamol is an independent risk factor for adult-onset asthma.     

Prenatal diagnosis of glucose-6-phosphate-dehydrogenase deficiency.

Prior to the development of the DNA-based technology reliable prenatal diagnosis of G6PD deficiency was not possible. We show that, using PCR amplification and restriction endonuclease digestion, prenatal diagnosis is possible. We have now been able to determine that the male fetus of a mother heterozygous for G6PD Mediterranean had inherited the maternal X chromosome with the normal G6PD gene.