兔肝VX_2肿瘤模型的建立及生物学特性

目的探讨采用瘤块种植制备兔肝VX2肿瘤模型的可行性并研究其生物学特性。方法采用VX2肿瘤组织块制备兔肝肿瘤模型,观察谷丙转氨酶(ALT)、体温、体质量、体液免疫球蛋白、肿瘤的增长、脏器转移及组织病理学改变等。结果兔肝VX2肿瘤原位移植模型的移植成功率为100%,6周肝内转移率为100%,6周肺转移率为80%。随着瘤体体积的增长,体温及ALT逐渐升高,体质量逐渐减轻,免疫球蛋白则呈下降趋势。超声检查显示肝脏有结节样增强回声。肿瘤呈灰白色,质硬,光镜下可见癌细胞核大,深染伴核异型。结论采用瘤块种植制备兔肝VX2肿瘤模型,具有容易制备、生长周期短、成功率高及模型稳定等特点,是一种较理想的肝肿瘤实验动物模型。     

正常青春期少女及特发性中枢性性早熟女孩生长激素结合蛋白、性激素与生长

本文对41例青春期女孩及30例特发性中枢性性早熟(ICPP)女孩。观察其身高、体重、身高增长速度、骨龄与生长激素结合蛋白(GHBP)、雌二醇(E_2)的相关关系,结果示GHBP在青春各期间无显著差异,GHBP与身高生长速度(GV)、身高标准差分(HtsDs)、体重指数(BMI)呈正相关,GHBP与E_2、年龄(CA)、骨龄(BA)无相关关系。正常青春少女与ICPP女孩相应青春期血清GHBP无明显差异,ICPP女孩GHBP与HtsDs及BMI呈正相关关系,与E_2、CA及BA无相关关系。提示正常青春少女及ICPP女孩的生长,在GH轴调控途径上,至少在生长激素受体(GHR)/GHBP水平是相同的。GHBP受营养的正性影响,不受年龄、骨龄影响。     

表麻下小切口透明晶状体吸出及人工晶状体植入术治疗高度近视

目的 :评价高度近视患者表麻下行透明晶状体吸出及人工晶状体植入术的术后效果。方法 :5 6例 10 6眼高度近视患者在表面麻醉下行小切口透明晶状体吸出及人工晶状体植入术。使用超声乳化仪 ,但未用超声能量。平均年龄 3 8 5 9± 9 44岁 ;术前裸眼视力 0 0 1～ 0 2 ,矫正视力 0 0 5～ 1 0 ;平均近视度 (-19 47± 5 41)D ,平均眼轴长(3 0 45± 1 94)mm ,平均植入人工晶状体屈光度数 ( 3 96± 4 2 7)D。术后平均随访时间 16个月。结果 :术后裸眼视力均好于或等于术前矫正视力。术后裸眼视力≥ 0 5者 79眼 (74 5 % )。术后矫正视力≥ 0 5者 99眼 (93 4% )。术后平均屈光度 (-1 49± 1 11)D。发生后囊膜混浊及玻璃体前界膜混浊 8眼 ,无视网膜脱离及黄斑囊样水肿发生。结论 :表麻下小切口透明晶状体吸出及人工晶状体植入术矫正高度近视 ,安全有效 ,预测性好 ,视力稳定。     

Ovarian hyperstimulation syndrome: a clinical retrospective study on 565 inpatients

Abstract

The aim of this study was to analyze clinical manifestations of 565 ovarian hyperstimulation syndrome (OHSS) inpatients in the largest Obstetrics and Gynecology hospital in China from year 2010 to 2017, to get more understanding of epidemiologic features of this disease, and to provide some insight on the diagnosis, treatment, and preventions of OHSS. It is a clinical retrospective study. In the 565 cases that developed OHSS over an eight-year period between 2010 and 2017 were reviewed, we assessed patients’ general characteristics, clinical manifestations, treatment, prognosis, and the relationship between different indicators and the severity of OHSS. Totally 12 kinds of ovulation induction protocols (Protocol 1: CC; Protocol 2: Gn; Protocol 3: hCG; Protocol 4: GnRh-a; Protocol 5: CC & Gn; Protocol 6: CC & hCG; Protocol 7: Gn & hCG; Protocol 8: GnRh-a & Gn; Protocol 9: CC & Gn & hCG; Protocol 10: GnRh-a & CC & Gn; Protocol 11: Letrozole & Gn & hCG; Protocol 12:GnRh-a & Letrozole & Gn) were analyzed and the Odds Ratio (OR) of each protocol were calculated. Five hundred and sixty-five patients were reviewed in our study. In all these patients, the number of hospitalizations, mean age, primary infertility rate, and pregnancy rate did not differ through the last 8?years. From which we may infer that the incidence rate of OHSS may not change over the last 8?years. Older patients tend to develop into more severe stage easily. The pregnancy rate was much lower in mild stage patients, but no difference was found between patients in moderate, severe and critical stage. Oocytes retrieval is strongly associated with severity. PCOS history, irregular menstrual cycle and infertility type do not seem to affect the severity of OHSS. Twelve kinds of ovulation induction protocols were analyzed, OR of different protocols were calculated, what is noteworthy is that patients who used GnRh easily developed more severe OHSS than the patients who received oocytes retrieval. We suggest that we may choose ovulation induction protocols according to the OR table while treating women with high-risk factors.     

手法治疗原发性膝关节骨关节病37例疗效观察

采用按摩膝关节痛点为主的手法，治疗原发性膝关节骨关节病３７例（４６个膝关节）．根据膝关节局部压痛点多少、髌骨活动程度是否异常、髌股关节磨擦疼痛程度、浮髌程度等体征和膝关节主动屈伸能力、行走能力等功能，对患者的严重程度进行评分和疗效判定。结果优１６膝、良１４膝、可１１膝、差５膝。笔者认为膝关节原发性骨关节病是一种软骨及周围软组织的综合性病损。以点揉痛点为主的手法具有促进病变部位组织血液循环，松解髌骨周围软组织粘连，恢复纤维组织弹性，增加髌骨活动度，减小髌股关节压力，促进膝关节软骨新陈代谢等作用。     

强心冲剂对充血性心力哀竭家兔模型心钠素、血管紧张素Ⅱ的影响

目的观察强心冲剂干预后充血性心力衰竭(CHF)家兔模型心钠素(ANP)、血管紧张素Ⅱ(AngⅡ)的变化,探讨强心冲剂对神经内分泌活性的影响.方法将40只家兔随机分为4组,假手术组、模型组、地高辛组、强心冲剂组,每组10只.采用腹主动脉缩窄术建立家兔CHF模型,予强心冲剂和地高辛灌胃,检测并比较ANP、AngⅡ的活性.结果CHF家兔模型ANP、AngⅡ活性显著升高,神经内分泌激素释放失衡,强心冲剂干预后,可以纠正其失衡状态,明显降低ANP、AngⅡ水平.结论强心冲剂能显著纠正神经内分泌激素释放失衡状态,改善神经内分泌激素的激活,可以防止CHF的进一步恶化.     

Research on Dynamic Compressive Performance and Failure Mechanism Analysis of Concrete after High Temperature and Rapid Cooling

To investigate the dynamic compressive properties of concrete after high temperature and rapid cooling, an experimental study was carried out by considering five temperatures and four strain rates. The coupling effect of high temperature and strain rate on concrete damage morphology and mechanical parameters was comparatively analyzed. The main conclusions are as follows: the compressive damage morphology of concrete is affected by strain rate development trends of significant variability under different temperature conditions. As the strain rate increases, the compressive stress and elastic modulus of concrete are gradually increased. As the temperature increases, the increase in compressive stress is gradually reduced by the strain rate. For the temperatures of 20 °C and 800 °C, the increase in compressive stress by the strain rate is 38.69% and 7.78%, respectively. Meanwhile, SEM and CT scanning technology were applied to examine the mechanism of the effect of high temperature and strain rate on the mechanical properties of concrete from the microscopic perspective, and the corresponding constitutive model was proposed.     

Using a two-step method of surface mechanical attrition treatment and calcium ion implantation to promote the osteogenic activity of mesenchymal stem cells as well as biomineralization on a β-titanium surface

Surface treatment is known as a very efficient measure by which to modulate the surface properties of biomaterials in terms of grain structure, topography, roughness and chemistry to determine the osseointegration of implants. In this work, a two-step method of surface modification was employed to impart high osteogenic activity and biomineralization capacity on a Ti–25Nb–3Mo–2Sn–3Zr alloy (a type of β-titanium named TLM). The preliminary surface mechanical attrition treatment (SMAT) refined the average grain size from 170 ± 19 μm to 74 ± 8 nm in the TLM surface layer and promoted the surface to be much rougher and more hydrophilic. The subsequent Ca-ion implantation did not change the surface roughness and topography obviously, but enhanced the surface wettability of the SMAT-treated TLM alloy. The in vitro evaluations of the adhesion, proliferation, osteogenic genes (RUNX2, ALP, BMP-2, OPN, OCN and COL-I) and protein (ALP, OPN, OCN and COL-I) expressions, as well as extracellular matrix (ECM) mineralization of mesenchymal stem cells (MSCs) revealed that the initial SMAT-treated sample significantly enhanced the adhesion and osteogenic functions of MSCs compared to an untreated TLM sample, and the subsequent introduction of Ca ions onto the SMAT-derived nanograined sample further promotes the MSC adhesion, proliferation, osteo-differentiation and ECM mineralization due to the adsorption of more proteins such as laminin (Ln), fibronectin (Fn) and vitronectin (Vn) on the surface, as well as the increase in extracellular Ca concentrations. In addition, the biomineralization capacity of the samples was also evaluated by soaking them in simulated bodily fluid (SBF) at 37 °C for 28 days, and the results showed that the Ca-ion implanted sample significantly boosted the deposition of Ca and P containing minerals on its surface, which was associated with the generation of more Ti–OH groups on the surface after ion implantation. The combination of the SMAT technique and Ca-ion implantation thus endowed the TLM alloy with outstanding osteogenic and biomineralization properties, providing a potential means for its future use in the orthopedic field.

Combination of the SMAT technique and Ca-ion implantation produced a β-titanium alloy with a bioactive surface layer, which was proved to effectively promote the osteogenic activity of MSCs and Ca–P mineral deposition in vitro.     

Efficacy analysis of Cheng’s GIRAFFE reconstruction after proximal gastrectomy for adenocarcinoma of esophagogastric junction

ObjectiveReconstruction of the digestive tract for adenocarcinoma of esophagogastric junction (AEG) is in dispute. This study evaluated Cheng’s gastric tube interposition esophagogastrostomy with reconstruction of His angle and fundus (Cheng’s GIRAFFE anastomosis) in laparoscopic/open proximal gastrectomy for Siewert type II AEG, which was performed at Zhejiang Cancer Hospital and the First Affiliated Hospital of Zhejiang Chinese Medical University. Here, we discuss the preliminary results of gastric emptying and anti-reflux.MethodsFrom a retrospective database, 74 patients with advanced Siewert type II AEG underwent curative proximal gastrectomy with GIRAFFE anastomosis, and their gastric emptying and anti-reflux outcomes were evaluated by the Reflux Disease Questionnaire (RDQ) score, nuclide gastric emptying, 24-h impedance-pH monitoring and gastroscopy.ResultsSeventy-four patients successfully completed proximal partial gastrectomy with Cheng’s GIRAFFE esophagogastric anastomosis. RDQ score six months after the operation was 2.2±2.5. Results of nuclide gastric emptying examinations showed that the gastric half-emptying time was 67.0±21.5 min, the 1-h residual rate was (52.2±7.7)%, the 2-h residual rate was (36.4±5.1)%, and the 3-h residual rate was (28.8±3.6)%; 24-h impedance-pH monitoring revealed that the mean DeMeester score was 5.8±2.9. Reflux esophagitis was observed by gastroscopy in 7 patients six months after surgery.ConclusionsCheng’s GIRAFFE anastomosis is safe and feasible for Siewert type II AEG.