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91.
Lei Xu You-Ming Li Chao-Hui Yu Lan Li You-Shi Liu Bao-Feng Zhang Jing Fang Qiong Zhou Ying Hu Hen-Jun Gao Department of Gastroenterology First Affiliated Hospital Zhejiang University School of Medicine Hangzhou China National Engineering Center for Biochip Shanghai China 《Hepatobiliary & Pancreatic Diseases International》2006,(1)
BACKGROUND: Pancreatic cancer development and progression is driven by the accumulation of genetic changes. In this study we constructed tissue microarray containing specimens from pancreatic cancer, adjacent non-cancer tissue and normal tissue to survey the expression of p53, p16 and cyclooxyganase-2 (COX-2). METHODS: Tissue microarray containing 337 specimens from different stages of pancreatic cancer, adjacent noncancer tissue and normal tissues was constructed, and the expression of p53, p16 and COX-2 was assayed by immunohistochemistry to consecutive formalin-fixed tissue microarray sections. RESULTS: The expression of p53, p16 and COX-2 was significantly higher in tumorous tissues than in non-tumorons ones. A significant relationship was observed between p53 and COX-2, or p16 and COX-2. But no obvious correlation was seen between p53 and p16 expressions. Logistic regression analysis showed p53 and COX-2 as dependent predictors in pancreatic carcinogenesis, and a reciprocal relationship to neoplastic progression between p53 and COX-2. CONCLUSION: Combination analysis of p53 and COX-2 may be useful in predicting pancreatic carcinogenesis. 相似文献
92.
Zhong-Yang Shen Zhi-Jun Zhu Yong-Iin Deng Hong Zheng Cheng Pan Ya-Ming Zhang Rui Shi Wen-Tao Jiang Jian-Jun Zhang Orient Organ Transplant Center First Central Hospital Tianjin China 《Hepatobiliary & Pancreatic Diseases International》2006,(2)
BACKGROUND: Because the orthotopic liver transplantation (OLT) was performed widely in recent 5 years throughout China, the proportion of recipients whose graft function deteriorated to be retransplantation candidates increased gradually. This study was undertaken to analyze clinical experience of orthotopic liver retransplantation (re-OLT) at our center. METHODS: The medical records of 80 patients who had undergone liver retransplantation at our center from January 1999 to July 2005 were analyzed retrospectively, including indications and timing of retransplantation, surgical techniques, and the causes of death. RESULTS: The commonest cause leading to hepatic graft loss and subsequent retransplantation was biliary complications in 36 patients (45%). The patients underwent retransplantation more than 30 days after their primary transplant recovered better than those who underwent retransplantation within 8-30 days after primary transplantation (peri-operative mortality 19.6% versus 70%). Sepsis (12 of 22 patients, 54.5%) and multiple organ failure (4 of 22 patients, 18.2%) were leading causes of re-OLT recipient deaths. CONCLUSIONS: Proper indications and optimal operative time, surgical procedures, perioperative monitoring and appropriate postoperative treatment contribute to the improvement of the survival rate of patients after liver retransplantation. 相似文献
93.
Jian-Xin Zhang Sheng-Chun Dang Jian-Guo Qu Xue-Qing Wang Guo-Zuo Chen Department of General Surgery The Affiliated Hospital of Jiangsu University Zhenjiang Jiangsu Province China 《World journal of gastroenterology : WJG》2005,(23)
AIM: To explore the relationship between gastric and intestinal microcirculatory impairment and inflammatory mediators released in rats with acute necrotizing pancreatitis (ANP). METHODS: A total of 64 rats were randomized into control group and ANP group. ANP model was induced by injection of 5% sodium taurocholate under the pancreatic membrane. Radioactive biomicrosphere technique was used to measure the gastric and intestinal tissue blood flow at 2 and 12 h after the induction of ANP, meanwhile serum phospholipase A2 (PLA2) activities and interleukin-1β levels were determined. Pathologic changes in pancreas, gastric and intestinal mucosae were studied. RESULTS: The gastric blood flow in ANP group (0.62±0.06 and 0.35±0.05) mL/(min·g) was significantly lower than that in control group (0.86±0.11 and 0.85±0.06) mL/(min·g) (P<0.01) at 2 and 12 h after induction of ANP. The intestinal blood flow in ANP group (0.80±0.07 and 0.50±0.06) mlV(min·g) was significantly lower than that in control group (1.56±0.18 and 1.61±0.11) mL/(min·g) (P<0.01). Serum PLA2 activities (94.29±9.96 and 103.71± 14.40) U/L and IL-1β levels (0.78±0.13 and 0.83±0.20)μg/L in ANP group were higher than those in control group (65.27±10.52 and 66.63±9.81) U/L, (0.32±0.06 and 0.33±0.07)μg/L (P<0.01). At 2 and 12 h after introduction of the model, typical pathologic changes were found in ANP. Compared with control group, the gastric and intestinal mucosal pathologic changes were aggravated significantly (P<0.01) at 12 h after induction of ANP. Gastric and intestinal mucosal necrosis, multiple ulcer and hemorrhage occurred. CONCLUSION: Decrease of gastric and intestinal blood flow and increase of inflammatory mediators occur simultaneously early in ANP, both of them are important pathogenic factors for gastric and intestinal mucosal injury in ANP. 相似文献
94.
Hui Lu Chao Yan Yi Zhang Bing-Ya Liu Shanghai Institute of Digestive Surgery Rujin Hospital Shanghai Second Medical University Shanghai China Zheng-Gang Zhu Xue-Xin Yao Ren Zhao Hao-Ran Yin Yan-Zhen Lin Department of General Surgery Rujin Hospital Shanghai Second Medical University Shanghai China 《World journal of gastroenterology : WJG》2005,11(14)
AIM: To provide hepatic protection through administration of doxorubicin before stop-flow chemotherapy (SFC) and to investigate the expression of heat shock protein 72 (HSP72) and role of nuclear factor kappa B (NF-κB) in this effect. METHODS: The hepatic preconditioning of doxorubicin was established in a porcine model by injection of doxorubicin (1 mg/kg) before SFC. The experimental animals were randomized into two groups: groups receiving doxorubicin (DOX) and normal saline (NS). Serial serum and tissue samples were taken from both groups to evaluate the protection of doxorubicin. Western blot and immunoprecipitation were applied to detect the expression of HSP72, NF-κB p65 protein, inhibitor κB-α (IκB-α) and phosphorylated IκB-α as well. The expression of tumor necrosis factor α (TNF-α) was estimated by semiquantitative RT-PCR. And the extent of the hepatic injury was estimated with the level of serum aminotransferases. RESULTS: An abundance production of HSP72 in porcine liver was observed after 24 h of intravenous administration of doxorubicin, but without any change in the expression of NF-κB p65 subunit in cytoplasm. NF-κB p65 subunit accumulated in nuclei at the end of SFC and reached its highest level at 30 min after the restoration of the abdominal circulation and decreased gradually during the 6 h after SFC in NS group, while there was little change in DOX group. There was also a slight decrease of IκB-α at 30 min after the restoration of the abdominal circulation in NS group accompanying with the appearance of phosphorylated IκB-α. The expression of TNF-α was significantly higher in NS group than that in DOX group (average 1.40±0.17 vs 0.62±0.22, P<0.01) at serial time points after SFC. Serum ALT and AST levels of NS group were higher after 24 h than those of DOX group (93.2±7.8 IU/L vs 53.3±13.9 IU/L, 217.0±29.4 IU/L vs 155.0±15.6 IU/L for ALT and AST respectively, P<0.05) and after 48 h than those of DOX group (66.6±18.1 IU/L vs 43.3±16.7 IU/L, 174.4±21.3 IU/L vs 125.7±10.5 IU/L for ALT and AST respectively, P<0.05). CONCLUSION: Doxorubicin renders the liver to be tolerant to the hepatic influence in SFC in a porcine model through the NF-κB/IκB-αpathway with the expression of HSP72. 相似文献
95.
DIFFEKENTIALCALCIUM-BINDINGABILITYOFFREEANDBOUNDPROTEINS-SUGGESTIONOFAN0VELPROTEINS/C4B-BINDINGPKOTEIN-BIND-INGFACTORINHUMANP... 相似文献
96.
Du Ru-yu杜如昱 Li Shu-duo李树铎 Huang Cui-ting黄萃庭 Guo Qian-xin郭钤新and Shi Wei-ming施维明People''s Hospital Beijing Medical Col.lege Beijing 《中华医学杂志(英文版)》1984,97(5):317-321
55 patients (24 were male and 31 female; age
range 12-'75 years) with Crohn's disease were
treated surgically. The lesions were single in 32
(58.2%) and multiple in 23 (41.8To). The distribu-
tion was: the small intestine, 26 patients (48.2'70);
the colon 20 (35.7%); and both 9 (16.170). Most
patients were opera.te{l upon fo;r a.cute abdo-
minal pain, intestinal obstruction, abdominal
mass and intestinal bleeding. Five patients (9.1%)
died during ho.spitalizatioin, 49 were followed up
for 0.5 t0 32 years;. Clinical cure was obtained
in 22 (54.6To) of the resection group and in 4
(30.7%) of the nonresection group. Indications
for surgical treatment are described and com-
plications discussed. 相似文献
97.
Zheng Tian-lin郑天林 Li De-gao李德高and Zhang Zhi-nan张之南Capital. Hospital BeijingCao Jin-ni曹金霓and Li Guo-xuan李国宣First Central. Hospital Tianjin 《中华医学杂志(英文版)》1985,98(5):321-324
This paper deals with clinical manifestations and
hematological findings in 71 cases of bone marrow
metastatic cancer. In all patients, the diagnosis of
malignant diseases was not made until bone marrow
aspiration was examined. In contrary to what was
reported in the literature, in our cases metastatic
cancer cells were not found more easily in bone mar
row biopsy than in aspirates. We consider combina
tion of biopsy with aspirates at multiple sites a better
way for obtaining positive findings. The morpho-
logical characteristics of metastatic cancer cells and
nonspecific myeloproliferative reaction to metastasis
are described, and differential diagnosis between
cancer cells and atypical marrow cells discussed. 相似文献
98.
Javier A Montero Hortensia Sánchez-Tocino Maria Josefa Martí Maldonado Concepci"n Arnaiz Nio Rosa Maria Pérez Bartolom Antonio Valera Coomonte Pio del Rio Hortega University Hospital Ophthalmology Unit Valladolid Spain Alicante Institute of Ophthalmology VISSUM Retina Unit Alicante Spain Albacete Medical School Castilla La Mancha University Spain 《国际眼科杂志》2008,8(6):1070-1072
目的:报告在西班牙Castilla-Leo地区患有渗出性老年性黄斑变性(AMD)的样本人群的流行病学特点,并与一组来自同一地区和相同年龄人群进行比较。方法:在本次观察性前瞻性调查中,我们访问了在一家地区性诊所接受光动力学疗法的AMD患者。患者报告了高血压、高血脂、吸烟的病史,检查患者虹膜颜色并分为浅色(绿色、蓝色、灰色)和深色(淡褐色、褐色、黑色)。结果:共有343名患者接受了访问,平均年龄为74.9岁(55~93岁),其中64.5%为女性,35.5%为男性,有45.1%的患者虹膜颜色被划为浅色。这些患者中,有50%有动脉性高血压,15.3%接受高胆固醇血症治疗,30.2%吸烟或者已经戒烟(女性中占80.9%)。结论:在渗出性AMD患者中,虹膜颜色为浅色的频率较高。在我们的调查中,其他渗出性AMD其他的风险因素有男性的吸烟习惯、未接受高胆固醇血症治疗、患者为女性身份等。 相似文献
99.
Mingyao Wu Liping Kuang Chaoxia Li Department of Pathology the Key Immunopathology Laboratory of Guangdong Province Shantou University Medical College Shantou China Guangzhou Red Cross Hospital Guangzhou China 《中德临床肿瘤学杂志》2007,6(5):503-506
We reported three cases of polypoid tumor of the esophagus, among them one case of sarcomatous tumor partly covered with superficial squamous cell carcinoma. The sarcoma was consisted of anaplastic spindle and pleomorphic tumor cells, which was similar to malignant fibrous histiocytoma (MFH) of the soft tissue. Diagnosis of the surgery resected speci-men was confirmed by histological, immunohistochemical and electron microscopic methods. Both diagnostic and differential diagnostic problems of primary MFH of the esophagus and world medical literatures were discussed. 相似文献
100.
COLORFLOWIMAGINGOFHEPATICCARCINOMAXuZhizhang;WangWenping(DepartmentofDiagnosticUltrasound,ZhongshanHospital,ShanghaiMedicalUn... 相似文献