VACUOLE SIGN AND SMALL NODE SIGN IN EARLY PERIPHERAL LUNG CANCER PATHOLOGIC BASIS AND DIAGNOSTIC VALUE

Radiologic and pathologic mani;estations of 30 peripheral pulmonary masses around l.5-3 cm in dia- meter were compared to the pathologic basis of va- cuole sign and small node sign on tomogram. Stati stics of 100 conventional X-ray tomograms of peri- pheral pulmonary masses about l-3 cm in diameter suggested that the vacuole sign and small node sign, more common in early peripheral lung cancer than in tuberculoma, are valuable for differentiating early peripheral lung cancer from tuberculoma.     

Effect of Sirpα1 on the expression of nuclear factor-kappa B in hepatocellular carcinoma

BACKGROUND:Signal regulatory protein alpha1(Sirpα1) is a member of Sirps families containing four immunoreceptor tyrosine-based inhibitory motifs(ITIMs) domains in the cytoplasm of and an activated substrate of receptor tyrosine kinase(RTK),that negatively regulates the RTK-dependent cell proliferating signal transduction pathway.Previously we found that Sirpα1 was closely associated with the occurrence and development of hepatocellular carcinoma(HCC)as well as liver regeneration.Since it is unclear about the regulatory mechanisms,we established the cell line transfected Sirpα1 gene and preliminarily clarified the mechanisms by which Sirpα1 negatively regulates the carcinogenesis and development of HCC. METHODS:Liver cancer Sk-Hep1 cell was respectively transfected with plasmids of pLXSN,pLXSN-Sirpα1 and pLXSN-Sirpα1Δ4Y 2 ,screened with the drug of G418(1200 μg/ml),and various transfected Sk-Hep1 cell lines were obtained.The protein expressions of P65,P50,IκBα,cyclin D1 and Fas in various Sk-Hep1 cell lines were determined by Western blotting,and P65 and P50 were localized by the immunofluorescence technique. RESULTS:Sirpα1 could significantly upregulate the protein expression of IκBα(vs.other cell lines,P<0.05) in the Sk-Hep1 cell,and downregulate the protein expressions of P65,P50 and cyclin D1(vs.other cell lines, P<0.05)in the Sk-Hep1 cell.P65 protein expression was mainly localized in the cytoplasm in the pLXSN Sk-Hep1 cell,and in the nucleus of the Sk-Hep1 cell with mutantSirpα1Δ4Y 2 ,but in nucleus of the Sk-Hep1 cell with wild Sirpα1.P50 protein expression was localized in the cytoplasm and nucleus of the pLXSN Sk-Hep1 cell,but in the nucleus of the Sk-Hep1 cell with wild Sirpα1 and mutant Sirpα1Δ4Y 2 plasmid. CONCLUSIONS:Sirpα1 might negatively regulate and control the abnormal proliferation of liver cancer cells by influencing the protein content and localization of nuclear factor-kappa B,then influence the expression of cyclins such as cyclin D1 in the signal transduction pathway.It may be one of the important mechanisms by which Sirpα1 negatively regulates the carcinogenesis and development of HCC.     

Beneficial Effects of Short-term Vasopressin Infusion during Severe Septic Shock

Background: Septic shock is associated with vasopressin deficiency and a hypersensitivity to its exogenous administration. The goal of the current study was to determine whether short-term vasopressin infusion in patients experiencing severe septic shock has a vasopressor sparing effect while maintaining hemodynamic stability and adequate end-organ perfusion.

Methods: Patients experiencing septic shock that required high-dose vasopressor support were randomized to a double-blinded 4-h infusion of either norepinephrine (n = 11) or vasopressin (n = 13), and open-label vasopressors were titrated to maintain blood pressure. To assess end-organ perfusion, urine output and creatinine clearance, gastric mucosal carbon dioxide tension, and electrocardiogram ST segment position were measured.

Results: Patients randomized to norepinephrine went from a median prestudy norepinephrine infusion of 20.0 [mu]g/min to a blinded infusion of 17.0 [mu]g/min at 4 h, whereas those randomized to vasopressin went from a median prestudy norepinephrine infusion of 25.0 [mu]g/min to 5.3 [mu]g/min at 4 h (P < 0.001). Mean arterial pressure and cardiac index were maintained in both groups. Urine output did not change in the norepinephrine group (median, 25 to 15 ml/h) but increased substantially in the vasopressin group (median, 32.5 to 65 ml/h;P < 0.05). Similarly, creatinine clearance did not change in the norepinephrine group but increased by 75% in the vasopressin group (P < 0.05). Gastric mucosal carbon dioxide tension and electrocardiogram ST segments did not change significantly in either group.     

RETlNAL VEIN OCCLUSION TREATMENT WITH WESTERN MEDICINE AND TRADITIONAL CHINESE MEDICINE

This paper presents 205 ca.ses of retinal vein occlusion (RVO) treated rr.wording t0 3 regi- mens and followed up periodically during the past 14 years. The clinical findings are analyz- ed. The effective rate of combined treatnwat with traditional Chinese medicine (TCM) and westeirn medicine in retinal branch vein oicclu- sion (RBVO) is 85.18To. 31.76'70 0f the patients with central retinal velin occlus.ion and 53.04% of tho相似文献   

LIAN Fang (连 方)—An outstanding gynecologist

LIAN Fang,was born in Rongcheng City,Shandong Province in September 1957. In December 1982,she received her bachelor's degree. She has been engaged in clinical practice,scientific research and teaching at the Department of Gynecology,the Affiliated Hospital of Shandong University of Traditional Chinese Medicine.During this period,she received her master's degree from the Department of Gynecology of Integrated Chinese and Western Medicine in Nanjing     

SIDE-TO-SIDE MESOCAVAL SHUNT IN PORTAL HYPERTENSION

Eighty-five consecutive patients underwent sideto.side mesocaval shunt with a success rate of 98.8'7。and 30day hospital mortality of 2.5"/o. Of 80 casesfollowed up for 3 months t0 8 years, 8 died, 3 hadencephalopathy, 5 rebleeding, 2 ascites and 3 lowerleg edema. A long history of hepatitis and liverfunction impairment were common features of dyingpatients. Rebleeding rate was 8.7'70. Liver functionplayed an important role in the determination of thetherapeutic effect.     

CLINICOPATHOLOGICAL CONFERENCE A 51-YEAR-OLD MAN WITH PALPITAT10N, SHORT BREATH, CYANOSIS AND SUFFOCATION

A 51-year-old man was admitted to our hospital on January 23, 1985 because of pal- pitation and short breath for 40 years. He was well until 7 years of age. Then he devel- oped symptoms ofpalpitation and shortbreath with cyanosisafter exercises. Relief of the symptoms would be achieved by squat- ting for a while or taking a few minutes' rest. Later, his growth was retarded. In 1960 he developed clubbing fingers. In 1965 when hospitalized for tonsillectomy, he was sus- pected of having "congenital heart disease" with ventricular septal defect, stenosis of the pulmonary artery and Eisenmengeris syndrome in question. In 1966, cardiac catheterization was performed, showing that the partial pressure of blood oxygen was l.85 vol%, higher in the right ventricle than in the left auricle. This means there exists a left to right shunt and the magnitude of shunt is 24.0% of the pulmonary circulation. The blood oxygen saturation in the f'emoral artery is a little bit low (93.5%). The hemoglubin concentration is (180g/l). Bilateral shunt could not be excluded.Since August 1969, he began to be very often troubled by nocturnal dyspnea, cough and chest distress. These symptoms could be abated by the treatment of aminophytlline. In 1976, ultrason- ocardiography revealed ventricular septal de- fect and aortic insufficiency. In May 1984. he had high fever for several days and was sus- pected of having "bacterial endocarditisu. The fever subsided with antibiotic therapy for a few db.ys. Twenty days before this hosptalization he had chest distress, short breath, palpitation, cyanosis, nocturnal dyspnea and paroxysmal precordial pain af- ter getting cold.     

TREATMENT OF HYALINEMEMBRANE DISEASE BYHUMAN AMNIOTIC FLUIDSURFACTANT

A case of hyaline membrane disease was treated successfully with pulmonary surfactant (PS) isolated from human amniotic fluid. Dosage was 150 mg phospholipid/kg. The exogenous surfactant was instilled into the airway via a tracheal cannula. Clinical symptoms, PO2 and FiO2 improved evidently 24 hours after administration. L/S ratio and phosphatidylglycerol recovered gradually in aspirates. Lung X-ray film manifested "white lung" before instillation of surfactant and showed a striking improvement 3 days after treatment. The duration of mechanical ventilation was 6 days. During the period of recovery complications of patent ductus arteriosus and bacterial pneumonia developed. However, the patient recovered completely and was discharged 32 days after admission.