平阳霉素治疗血管瘤、血管畸形后组织学观察

目的 :观察平阳霉素治疗血管瘤、血管畸形后引起的组织学变化。方法 :13例不同类型的血管病变 ,分1～ 3次注射平阳霉素 4～ 2 4mg ,多次注射者间隔 10～ 12d .在末次注射后 12～ 15d切除治疗后的病变组织进行组织学观察。结果 :注射 1次的标本中血管壁增厚 ,管腔变狭窄 ;注射 2次的标本中管腔闭塞 ,管壁失去正常结构 ;注射 3次的标本中管壁结构消失 ,组织纤维化。结论 :平阳霉素瘤腔内注射后可引起血管内膜变化 ,致使管腔闭塞 ,从而治愈血管瘤及血管畸形     

带血管蒂锁骨内侧端骨膜、骨瓣移位术的应用解剖

目的 :为带血管蒂锁骨内侧端骨膜、骨瓣转位术提供解剖学基础。方法 :在 30侧成人尸体标本上 ,观察了甲状腺上动脉胸锁乳突肌支的走行、分支、分布及吻合 ,并在标本上模拟术式设计。结果 :恒定起源的甲状腺上动脉胸锁乳突肌支游离长度达 8cm以上 ,外径 1.4± 0 .3mm。 90 .0 %终支直接达锁骨 ,10 .0 %与颈横动脉 (肩胛上动脉 )锁骨支吻合间接达锁骨。结论 :以甲状腺上动脉胸锁乳突肌支为蒂锁骨内侧端骨膜、骨瓣转位修复下颌骨缺损或行颈椎前方植骨融合具有可行性。     

ClinicalStudy on Zhenjian Granule (针箭颗粒) in Improving Essential Hypertension and InsulinResistance

Objective: To observe the effect of Zhenjian Granule (ZJG) in improving essential hypertension and insulin resistance (IR).Methods: The 52 patients of hypertension were randomly divided into the ZJG group (n=32) and the control group (n=20), treated with ZJG and captopril respectively for 4 weeks. The changes of blood pressure, glucose, insulin, insulin sensitivity index (ISI) and blood lipid in them before and after treatment were determined and compared with those in normal group (17 healthy subjects).Results: Fasting insulin, blood glucose, and insulin 2 hrs after meal in hypertension patients before treatment were significantly higher but ISI was significantly lower than those in the normal control, indicating the existence of insulin resistance. After treatment, not only blood pressure in patients obviously lowered, but also levels of fasting insulin, blood glucose, and insulin 2 hrs after meal obviously lowered, while ISI markedly raised. IR was improved in both groups, and blood lipid were also improved in the ZJG group.Conclusion: ZJG was effective in reducing blood pressure, also it had significant effect in improving metabolic disorder, particularly on IR in patients of essential hypertension.     

Effect of xiongshao capsule on vascular remodeling in porcine coronary balloon injury model

Objective: To study the effect of Xiongshao capsule (XSC) on vascular remodeling in porcine coronary balloon injury model.Methods: Coronary artery restenosis model was established by oversized balloon injury at mid-region of the left anterior descending coronary artery. They were divided into 5 groups, untreated or treated with probucol, Xueguantong, low and high dose of XSC respectively, and compared. The effect of XSC on late lumen loss resulted from vascular remodeling was evaluated by quantitative histological method with combining histopathological analysis and coronary angiographie examination.Results: The (59 ± 20)% of late lumen loss was caused by vascular remodeling and (41 ± 20)% caused by intimai hyperplasia. Compared with the control group, all the treatment could significantly reduce the late lumen loss after balloon injury (P<0.05 orP < 0.01 ), both low and high dose of XSC could significantly reduce the late lumen loss caused by remodeling (P<0.05 and P<0.01 respectively).Conclusion: Vascular remodeling plays an important role in late lumen stenosis formation in coronary artery after balloon injury. XSC could significantly inhibit the pathological vascular remodeling, and thus reduce the late lumen loss and prevent the restenosis of the injured coronary artery. Part of a National Ninth-Five Planning Project (No. 96-9060601)     

Study on early application of chinese medicinal herbs after total gastrectomy

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BCL-XL异质性表达与复发急性髓性白血病细胞体外生长类型的关系

选择１５例复发急性髓性白血病（ＡＭＬ）患者,研究ＢＣＬ－ＸＬ的异质性表达与复发ＡＭＬ细胞体外生长类型的关系。用白血病祖细胞培养（ＣＦＵ－Ｌ）判断生长类型,ＲＴ－ＰＣＲ方法检测 ＢＣＬ－ＸＬ ｍＲＮＡ的表达丰度。结果：ＣＦＵ－Ｌ检测中,１０例为生长型,其ＢＣＬ－ＸＬ ｍＲＮＡ ９例高表达,１例为低表达;ＣＦＵ－Ｌ检测 ５例为不生长型,ＢＣＬ－ＸＬ ｍＲＮＡ均为低表达（ Ｐ＜０．０１）。在复发ＡＭＬ中,ＢＣＬ－ＸＬ ｍＲＮＡ的高表达与其细胞体外生长类型有关,提示与其细胞的自分泌生长有关,可以作为复发ＡＭＬ再生耐药的一个判断指标。     

椎基底动脉短暂缺血性眩晕患者脑血流量与脑干听力诱发电位的关系研究

为探讨椎基底动脉短暂缺血性眩晕 (VBTIV)患者的脑干听力诱发电位 (BAEP)与脑血流量 (CBF)和病理改变的关系 ,对 6 5例 VBTIV患者 (患者组 )作磁共振血管成像 (MRA)、经颅多普勒 (TCD)和 BAEP检查 ,对 2 6例对照组患者作 MRA和 TCD检查。结果 :MRA显示患者组 5 0例血管病理改变明显 ,1 5例改变不明显或无改变 ;对照组脑血流量为 1 1 2 .3～ 2 78.9ml/ min(2 s) ,较患者组 48.0～ 2 6 2 .0 m l/ min高 (P<0 .0 1 ) ,患者组 1 5例脑血流量下降 ,5 0例脑血流量正常 ;患者组 BAEP异常 47例 (72 .3% ) ,其中 ,1 5例脑血流量下降者 ,1 4例(93.3% ) BAEP异常 ,比 5 0例脑血流量正常者中的 33例 (6 6 % ) BAEP异常高 (P<0 .0 5 ) ;5 0例血管病理改变明显者 ,BAEP异常 40例 (80 % ) ,比 1 5例血管病理改变轻或无改变者中的 7例 (43.3% ) BAEP异常高 (P<0 .0 5 ) ,说明BAEP与 CBF和血管病理改变密切相关     

雌激素和异黄酮类药物对内皮细胞粘附分子CD54表达的影响

为探讨雌激素和异黄酮类药物对内皮细胞粘附分子CD54表达的影响,作者采用10ng/ml肿瘤坏死因子(TNFα)处理人脐静脉内皮细胞6小时,并加用不同浓度的异黄酮(WZ1,WZ2)和雌激素(WZ3,WZ4)进行干预48小时,而后用流式细胞仪定量测定细胞表面粘附分子CD54的表达,并进行统计学分析.结果:①10ng/ml的TNFα可使内皮细胞表面粘附分子CD54的表达升高4倍,与对照组相比具有统计学差异(P＜0.05);②10-10mol/L、10-8mol/L、10-6mol/L异黄酮和异卟黄酮对由TNFα诱导的内皮细胞的CD54表达有轻度促进作用;③哌嗪雌酚酮和雌二醇预处理内皮细胞48小时后再加TNFα激活,内皮细胞表面CD54平均荧光强度与单纯TNFα组相比下降明显(P＜0.05),各浓度组之间不具统计学差异(P＞0.05).以上结果提示:异黄酮和异卟黄酮对内皮细胞粘附分子CD54的表达无抑制作用;哌嗪雌酚酮和雌二醇可在一定程度上抑制CD54的表达,从而为抗粘附疗法提供了一条新思路.     

子宫内膜癌雌、孕激素受体的检测及其临床意义

为探讨子宫内膜癌雌激素受体(ER)、孕激素受体(PR)检测的方法及其意义,用抗ER、PR单克隆抗体免疫组化SP法检测了36例子宫内膜癌石蜡切片ER、PR的表达,其中28例还用葡聚糖包裹活性炭法(简称DCC法)检测了ER、PR含量.结果显示,免疫组化SP法检测ER、PR阳性率分别为44.4%和52.8%,DCC法检测的阳性率分别为50%、57.1%.两种方法测定ER的符合率为60.7%,相关系数为0.565,测定PR的符合率为85.7%,相关系数为0.563(P值均<0.05).高、中分化的内膜癌ER、PR含量明显高于低分化的内膜癌.以上提示,ER、PR的表达与组织学分级有关,反映了肿瘤的生物学行为.免疫组化SP法和DCC法测定ER、PR有较好的相关性,两种方法联合应用将对预测临床预后、指导治疗有一定的意义.     

遗传性脊髓小脑型共济失调病人SCA_3基因突变检测

1目的　探讨遗传性脊髓小脑型共济失调 (SCA)病人 SCA3基因突变的意义。2方法　应用聚合酶链反应 (PCR)、聚丙烯酰胺凝胶电泳银染法 ,对临床诊断为 SCA的 15个家系的 32例病人及其有亲缘关系的 2 2例正常者 ,进行 SCA3基因内一段包含 CAG三核苷酸重复序列片段扩增。3结果　 5个家系中的 2 1例病人各有 1个 SCA3异常等位基因扩增片段 ,其中 1个家系中 2例病人和 1例“正常者”的 1个等位基因的 CAG三核苷酸重复数目都为 48次。4结论　检测 SCA基因的动态突变是目前诊断 SCA的唯一有效方法。