融合蛋白AML1-ETO对C/EBPα基因表达的影响

背景：已有研究显示AML1-ETO可以通过包括抑制C/EBPα的功能在内的机制引起分化的阻滞。目的：观察AML1-ETO对髓系分化相关基因C/EBPα表达的影响,探讨其在白血病发生中的作用。方法：应用流式细胞术检测急性单核细胞白血病细胞株U937、U937-MOCK和U937-A/E1细胞表面抗原CD11b、CD14的表达;荧光实时定量PCR检测C/EBPαmRNA的表达;免疫印迹法检测C/EBPα蛋白的表达;染色质免疫沉淀技术研究转染细胞中AML1-ETO与C/EBPα基因启动子之间直接的相互作用情况。结果与结论：AML1-ETO转染细胞的细胞表面分化抗原CD11b和CD14的表达明显下降,且转染了AML1-ETO的U937细胞系中,C/EBPα的mRNA与蛋白水平均下调,转染细胞沉淀富集的DNA中含有C/EBPα基因的启动子序列。结果提示C/EBPα是AML1-ETO的直接靶基因,AML1-ETO能下调C/EBPα的表达。     

PGC-1α基因多态性与冠心病的相关性分析

目的研究过氧化物酶体增殖物激活受体7共激活因子1α（PGC-1α）基因的单核苷酸多态性（single-nucleotidepolymorphism,SNP）住点rs2970847与中国汉族人群冠心痛（coronaryheartdisease,CHD）的相关性。方法本研究共选取中国汉族冠心病患者231人（男性151,女性80）,正常对照组478人（男性297,女性181）。采用限制性酶切片段长度多态性（Restriction Fragment Length Polymorphism,RFLP）的方法对rs2970847进行基因分型。并测定所有入选对象的体重指数、血糖、总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白等指标。结果冠心病患者中,rs2970847位点的CC基因型为144人（62．3％）,CT基因型为75人（32．5％）,TT基因型为12人（5．2％）;而正常对照中,CC基因型为235A-（49．2％）,CT基因型为201人（42．1％）,TT基因型为42人（8．8％）。rs2970847位点与冠心痛显著相关（P=0．0009）,纯合子CC基因型可以增加冠心病的患病风险（ORCC：2．14,95％CI=1．09-4．21）。结论PGC—1α基因多态位点rs2970847与冠心痛具有显著相关性,其CC基因型是冠心病的危险因素。     

Chemical composition and antiproliferative activity of essential oil from the leaves of a medicinal herb, Schefflera heptaphylla

Schefflera heptaphylla (L.) Frodin is a medicinal herb widely used as a main ingredient of the popular health tea formulation against infections in Southern China. Twenty-seven volatile compounds were identified by GC-MS analysis from the essential oil obtained from the leaves of S. heptaphylla, and 17 of them belonged to monoterpenes or sesquiterpenes. The main volatile constituent in S. heptaphylla was found to be a monoterpene, beta-pinene, comprising about 22% of the total volatile components. The essential oil showed significant antiproliferative activity against three cancer cell lines, MCF-7, A375 and HepG2 cells, with IC50 values of 7.3 microg/mL, 7.5 microg/mL and 6.9 microg/mL, respectively. The result of the cytotoxicity assay indicates that (-)-beta-pinene and (+)-beta-pinene (commercially available from Sigma) also possessed antiproliferative activity against the cancer cells MCF-7, A375 and HepG2 with IC50 values ranging from 147.1 to 264.7 microm.     

放血疗法适宜病症初探

探讨放血疗法的适宜病症,以指导临床更好地应用该疗法.采用回顾性期刊文献系列研究,检索中国知网中国期刊全文数据库、维普中文科技期刊全文数据库、万方数据库、中国生物医学文献数据库等4个数据库的放血疗法所有临床研究文献,统计放血疗法治疗疾病的文献频次、病例总数,运用临床流行病学和循证医学的原则和方法对文献进行分类分级,按照世界卫生组织关于疾病和有关健康问题的国际统计分类(ICD10)对疾病进行分类.检出有效文献1149篇,共计病例98 526例,共涉及18大类系统,261个病种.结果表明放血疗法的适宜病症广,值得推广,特别是对带状疱疹、痤疮、麦粒肿、颈椎病、口腔溃疡有明显的治疗优势.     

Clinical features of intracerebral hemorrhage in patients with colorectal cancer and its underlying pathogenesis

BACKGROUNDThe diagnosis of both cancer and intracerebral hemorrhage (ICH) in the same patient is not uncommon, but the clinical features and pathogenesis of patients with colorectal cancer (CRC) and ICH are still not well known.AIMTo investigate the clinical features and underlying pathogenesis of ICH in patients with CRC. METHODSA retrospective review of CRC patients complicated with ICH from three centers between January 2014 and December 2020 was performed. Clinical data such as laboratory examinations, imaging features, prognosis, and underlying pathogenesis were analyzed.RESULTSOf 16673 identified CRC patients, 20 (0.12%) suffered from ICH. There were 13 males and 7 females, with an average age (mean ± SD) of 68.45 ± 10.66 years. Fourteen patients (70%) had distant metastases and most patients (85%) showed an elevation of one or more cancer biomarkers. The hemorrhagic lesions in 13 patients (65%) were in the intracerebral lobe. Four patients were completely dependent and 4 died within 30 days after hemorrhage. Intratumoral hemorrhage (50%) and coagulopathy (50%) accounted for the majority of hemorrhages.CONCLUSIONPatients with ICH and CRC often have clinical features with lobar hemorrhage, distant metastases and poor prognosis. Intratumoral hemorrhage and coagulopathy are the main causes of ICH in patients with CRC.     

TGF-β1/Smad3信号转导通路与创伤后瘢痕形成

目的对近年TGF-β1/Smad3信号转导通路与创伤后瘢痕形成的相关研究作一综述。方法广泛查阅国内外近年有关TGF-β1/Smad3信号转导通路及与创伤后瘢痕形成的文献,并进行综述。结果 TGF-β1是纤维化疾病的重要影响因子,通过TGF-β1/Smad3信号通路转导产生其生物学效应。该途径受多种因子调控并在细胞及分子水平与其他信号通路串话。该途径参与创伤后早期炎性反应、创面愈合及后期病理性瘢痕的形成。在分子水平干预转导途径的各个环节,可以影响纤维化及细胞外基质沉着的进程。结论 TGF-β1/Smad3信号转导途径是影响创伤后瘢痕形成及细胞外基质沉着的重要途径。对该途径进行深入研究,可为临床促进创面的愈合及病理性瘢痕的防治奠定理论基础。     

子宫内膜间质肉瘤CT、MRI表现

目的分析子宫内膜间质肉瘤(ESS)的CT、MRI表现。方法回顾性分析经术后病理证实为ESS的11例患者的CT、MRI资料。11例中,5例接受CT检查,6例接受MR检查,均包括平扫及增强扫描。结果病理结果为低级别ESS 9例,未分化肉瘤2例。11例病灶均为单发,病灶位于宫腔内7例、肌壁间4例,其中类圆形肿块8例、不规则形肿块3例,病灶最大径(9.18±1.36)cm,肿块边界清晰4例、边界不清7例;病灶CT、MRI表现以实性为主4例、呈囊实性6例、以囊性为主1例。CT平扫示5例病灶实性部分密度均近似于或低于子宫肌层,其中4例病灶密度不均,1例密度均匀。MRI平扫示6例病灶ADC图信号均减低,其中5例呈T1WI等或低信号、T2WI高或稍高信号且DWI呈明显高信号,另1例(以囊性为主)呈T1WI稍高信号、T2WI高信号且DWI呈中心高信号。10例增强扫描表现为渐进性、持续性强化,1例以囊性为主的病灶未见强化。8例肿块内可见囊变、坏死,6例肿瘤浸润、破坏子宫深肌层。11例中,伴子宫腺肌症2例、子宫肌瘤5例,合并少量盆腔积液5例、宫腔内积血1例、输卵管积液2例。结论 ESS的CT、MRI表现具有一定特征,可为诊断提供依据。     

闭孔疝的诊断和治疗

目的探讨闭孔疝的诊治方法。方法对15例闭孔疝病例诊治经过结合相关文献进行回顾性分析。结果15例中,术前确诊率仅20%(3/15),误诊率80%(12/15),全组均行疝环修补术,采用剖腹探查切口。本组治愈率100%,术后切口感染及愈合不良4例(21.67%),随访8例至今无复发。结论术前易误诊、漏诊;对年老体弱、多胎生育伴有不明原因肠梗阻表现的妇女,应高度考虑闭孔疝,Howship-Romberg征及Hannington-Kiff征阳性可确诊。疝环修补术是惟一有效的方法,首选腹腔内入路。早确诊,及时手术是减少并发症、降低死亡率的关键。     

Facile synthesis of photoactivatable adenosine analogs

Adenosine and its derivatives are important building blocks of the biological system. They serve as the universal energy currency, amplify intracellular signals for various signal transduction pathways, and can also be used as the co-substrates for enzymatic transformations. The synthesis and regulation of adenosine and its analogs rely on the adenosine binding proteins (ABPs). Dysregulated ABP activity contributes to numerous diseases such as cancer, metabolic disorders, and neurodegenerative diseases. Presently, there is intense interest in targeting ABPs for therapeutic purposes. A large fraction of the human ABP family remains poorly characterized. The need for innovative chemical probes to investigate ABP function in the native biological matrix is apparent. In this study, an adenosine analog, probe 1, with a photoaffinity group and biotin tag was synthesized using concise synthetic strategies. This probe was able to label and capture individual recombinant ABPs with good target selectivity. Probe 1 was also evaluated for its ability to label spiked ABP in complex cell lysates. This chemical probe, together with the labeling and enrichment assay, is of great value to interrogate the biological functions of ABPs and to elucidate their diversity under different physiological conditions.

Photoactivatable adenosine analog-enabled capture and enrichment of adenosine binding protein (ABP).     

Molecular phenotypes reveal heterogeneous engraftments of patient-derived hepatocellular carcinoma xenografts

ObjectivePatient-derived xenograft (PDX) models provide a promising preclinical platform for hepatocellular carcinoma (HCC). However, the molecular features associated with successful engraftment of PDX models have not been revealed.MethodsHCC tumor samples from 76 patients were implanted in immunodeficient mice. The molecular expression was evaluated by immunohistochemistry. Patient and tumor characteristics as well as tumor molecular expressions were compared for PDX engraftment using the Chi-square test. The independent prediction parameters were identified by logistic regression analyses.ResultsThe engraftment rate for PDX models from patients with HCC was 39.47% (30/76). Tumors from younger patients and patients with elevated preoperative alpha-fetoprotein level had higher engraftment rates. Tumors with poor differentiation and vascular invasion were related to engraftment success. The positive expression of CK19, CD133, glypican-3 (GPC3), and Ki67 in tumor samples was associated with engraftment success. Logistic regression analyses indicated that GPC3 and Ki67 were two of the strongest predictors of PDX engraftment. Tumors with GPC3/Ki67 phenotypes showed heterogeneous engraftment rates, with 71.9% in GPC3⁺/Ki67⁺ tumors, 30.8% in GPC3⁻/Ki67⁺ tumors, 15.0% in GPC3⁺/Ki67⁻ tumors, and 0 in GPC3⁻/Ki67⁻ tumors. ConclusionsSuccessful engraftment of HCC PDXs was significantly related to molecular features. Tumors with the GPC3⁺/Ki67⁺ phenotype were the most likely to successfully establish HCC PDXs.