Improving microalgal growth by strengthening the flashing light effect simulated with computational fluid dynamics in a panel bioreactor with horizontal baffles

Biological CO₂ elimination by photosynthetic microalgae is a sustainable way to mitigate CO₂ from flue gas and other sources. Computational fluid dynamics was used to simulate algal cell movement with an enhanced flashing light effect in a novel panel bioreactor with horizontal baffles. Calculation results showed that the light/dark (L/D) cycle period decreased by 17.5% from 17.1 s to 14.1 s and that the horizontal fluid velocity increased by 95% while horizontal baffles were used under a 0.02 vvm air aeration rate and a microalgal concentration of 0.85 g L⁻¹. The probability of the L/D cycle period within 5–10 s increased from 27.9% to 43.6%, indicating a 56% increase when horizontal baffles existed. It was proved by experiments that the mass-transfer coefficient increased by 31% and the mixing time decreased by 13% under a 0.06 vvm air aeration rate when horizontal baffles were used, and the algal biomass yield increased by ∼51% along with the decrease in the L/D cycle period when horizontal baffles were used.

Biological CO₂ elimination by photosynthetic microalgae is a sustainable way to mitigate CO₂ from flue gas and other sources.     

Characteristics of negative DC discharge in a wire–cylinder configuration under coal pyrolysis gas components at high temperatures

This work aims to provide a comprehensive understanding of negative DC discharge under coal pyrolysis gas components (CO₂, H₂, N₂, CH₄, CO) and air. The characteristics of negative DC discharge were studied in a wire–cylinder configuration at an ambient temperature range of 20–600 °C by analyzing V–I characteristics, discharge photographs, and gas composition. With increasing temperature, corona onset voltage, spark breakdown voltage and operational voltage range for corona discharge decrease, but discharge current and electron current ratio increase. Discharge current of CO₂ is higher than that of air due to the difference of electronegativity. During CO₂ discharge, with the increase of output voltage, three types of discharge are successively observed, namely corona, glow and arc. However, during H₂ discharge, only glow discharge is observed. Temperatures significantly affect the capability of CO to attach electrons. The discharge characteristic of CO is similar to the electronegative gas media at 20 °C and the non-electronegative gas media when the temperature exceeds 350 °C. Chemical reactions and carbon generation are observed during the CH₄ and CO discharge process. The product of carbon filaments under the CH₄ gas medium leads to discharge current volatility and short circuit. These results assist in understanding the property of ESP at high temperatures.

This work aims to provide a comprehensive understanding of negative DC discharge under coal pyrolysis gas components (CO₂, H₂, N₂, CH₄, CO) and air.     

愈肝胶囊抗鸭乙型肝炎病毒作用实验研究

观察愈肝胶囊抗鸭乙型肝炎病毒作用。方法选1日龄广州麻鸭50只接种病毒,分ACV200μg/kg,愈肝胶囊6.5g/kg,3.25g/kg及病毒对照组,1/d,口服。分别观察其抗DHBV的作用。试验结束时,取肝组织作病理切片光镜观察。结果 6.5g/kg愈肝胶囊组在给药后第5天,第10天和停药后第3天,鸭血清DHBV DNA水平均明显下降,与给药前自身比及病毒对照组比较,差异均有显著性(P相似文献   

明胶酶A在急性白血病细胞中的表达及其临床意义

目的　探讨急性白血病 (AL)细胞中明胶酶A(MMP2 )的表达及其临床意义。方法　应用明胶酶谱法检测了 4 6例初治AL患者MMP2的表达 ,同时应用逆转录 PCR(RT PCR)测定了MMP2活化相关基因基质金属蛋白酶抑制剂 2 (TIMP2 )、基质金属蛋白酶 1(MT1 MMP)的表达 ,并在体外进行了白血病细胞株穿膜试验研究。结果　 4 6例AL患者中 2 4例 (5 2 2 % )MMP2表达阳性 ,MMP2阳性组与阴性组发生髓外浸润的比例分别为 5 0 0 %和 18 2 % (P值均 <0 0 5 ) ;外周血幼稚细胞百分率分别为 (77 2 1± 13 9) %和 (6 2 95± 17 2 ) % (P值均 <0 0 1)。同时 4 6例AL患者中TIMP2、MT1 MMP阳性表达分别为 2 7例 (5 8 7% )、33例 (71 7% )。体外穿膜试验证实了MMP2对白血病细胞侵袭能力的影响。结论　活性MMP2可能参与白血病细胞从骨髓释出并浸润组织的过程     

玉屏风制剂联合匹多莫德治疗儿童反复呼吸道感染的Meta分析

目的：系统评价玉屏风制剂联合匹多莫德治疗儿童反复呼吸道感染的临床疗效和安全性,为临床循证医学研究提供参考。方法：计算机检索PubMed、EMBase、中国生物医学文献数据库（CBM）、中国知网（CNKI）、维普数据库（VIP）和万方数据库,收集常规治疗+玉屏风制剂联合匹多莫德（试验组）对比单用常规治疗或常规治疗+匹多莫德（对照组）治疗儿童反复呼吸道感染（RRTI）的随机对照试验（RCT）,检索时间为2000年1月至2018年3月,提取资料并评价质量后,采用RevMan 5.2软件进行Meta分析。结果：共纳入12篇文献,1 175例患儿。Meta分析结果显示,试验组总有效率高于对照组（RR=1.25,95% CI 1.19~1.32,P<0.01）;退热时间、咳嗽缓解时间、肺部啰音和扁桃体红肿消失时间均短于对照组（P均<0.01）;治疗后免疫球蛋白水平（IgA、IgM和IgG）和T淋巴细胞水平（CD3+、CD4+和CD4+/CD8+）均高于对照组（P均<0.01）。所有文献均未报道不良反应。结论：玉屏风制剂联合匹多莫德能够显著改善儿童RRTI的免疫功能及临床体征,从而提高总有效率。     

Systemic Inflammatory Response Syndrome is Associated with Hematoma Expansion in Intracerebral Hemorrhage

ObjectivesSystemic inflammatory response syndrome (SIRS) and hematoma expansion are independently associated with worse outcomes after intracerebral hemorrhage (ICH), but the relationship between SIRS and hematoma expansion remains unclear.Materials and methodsWe performed a retrospective review of patients admitted to our hospital from 2013 to 2020 with primary spontaneous ICH with at least two head CTs within the first 24 hours. The relationship between SIRS and hematoma expansion, defined as ≥6 mL or ≥33% growth between the first and second scan, was assessed using univariable and multivariable regression analysis. We assessed the relationship of hematoma expansion and SIRS on discharge mRS using mediation analysis.ResultsOf 149 patients with ICH, 83 (56%; mean age 67±16; 41% female) met inclusion criteria. Of those, 44 (53%) had SIRS. Admission systolic blood pressure (SBP), temperature, antiplatelet use, platelet count, initial hematoma volume and rates of infection did not differ between groups (all p>0.05). Hematoma expansion occurred in 15/83 (18%) patients, 12 (80%) of whom also had SIRS. SIRS was significantly associated with hematoma expansion (OR 4.5, 95% CI 1.16 - 17.39, p= 0.02) on univariable analysis. The association remained statistically significant after adjusting for admission SBP and initial hematoma volume (OR 5.72, 95% CI 1.40 – 23.41, p= 0.02). There was a significant indirect effect of SIRS on discharge mRS through hematoma expansion. A significantly greater percentage of patients with SIRS had mRS 4-6 at discharge (59 vs 33%, p=0.02).ConclusionSIRS is associated with hematoma expansion of ICH within the first 24 hours, and hematoma expansion mediates the effect of SIRS on poor outcome.     

Benchmarking Various Radiomic Toolkit Features While Applying the Image Biomarker Standardization Initiative toward Clinical Translation of Radiomic Analysis

Journal of Digital Imaging - The image biomarkers standardization initiative (IBSI) was formed to address the standardization of extraction of quantifiable imaging metrics. Despite its effort,...     

QuECHERS-HPLC-MS/MS测定祛痘化妆品中7种硝基咪唑类抗菌药物

目的建立测定祛痘化妆品中硝基咪唑类抗菌药物甲硝唑、地美硝唑、洛硝哒唑、异并硝唑及其代谢物羟基甲硝唑、羟基二甲硝咪唑和羟基异丙硝唑的方法。方法试样用0.1%甲酸乙腈溶液提取,通过QuEChERS净化后过滤膜上机检测,以ZORBAX SB-C18色谱柱分离,0.1%甲酸乙腈-0.1%甲酸水溶液作为流动相梯度洗脱,电喷雾正离子模式(ESI+),多重反应监测(MRM)扫描,外标法定量。结果 7种目标分析物在5.0~500.0ng·mL^-1内与峰面积均呈良好线性关系,相关系数均>0.995,在3个不同浓度添加水平下,平均加样回收率为87.9%~114.9%,相对标准偏差RSD(n=6)为0.4%~4.5%。方法的定量限为5~40μg·kg^-1,最低检测限为1.4~12.0μg·kg^-1。结论该方法具有净化效果好、操作简单、准确快速的特点,适用于祛痘化妆品中硝基咪唑类抗菌药物及其代谢物的检测。