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71.
72.
Hasan Mujtaba Ying Wang Yixin Duan Meng Cao Nana Zhang Iffat Batool Ali Murtaza Xiaoli Chen Yili Wang 《Pathology, research and practice》2018,214(10):1713-1718
Background
The expected corresponding increase in tonsillar human papillomavirus (HPV) infection associated with increasing incidence of oropharyngeal squamous cell carcinoma (OPSCC) substantiate the evaluation of normal tonsillar tissue in different population. The epidemiology of HPV in tonsillar tissue varies geographically. This study evaluated samples from two countries to determine the prevalence in respective samples.Objective
To characterize HPV infection in non-malignant tonsillar tissue from Shaanxi, China (herein after referred to as China) and Punjab, Pakistan (herein after referred to as Pakistan).Methodology
The DNA extracted from formalin-fixed, paraffin-embedded tumor free tonsillar tissue was evaluated using polymerase chain reaction (PCR). A total of 367 cases from China and 139 cases from Pakistan were screened for HPV DNA using GP5+/GP6+ consensus primer. Genotype of the positive cases was determined for common HPV types (6, 11, 16, 18, 52, 58) simultaneously by type-specific PCR.Results
The mean age of cohorts in China was 13.42 (Median age 7, Range 2–72 years) while in Pakistan it was 10.77 (Median age 8, Range 3–42 years) the gender distribution was 61.6% male in China and in Pakistan they were 56.8%, rest were females. The overall prevalence of HPV in China was 2.45% and 2.16% in Pakistan. High risk human papillomavirus was 1.63% in China with 5 cases positive for HPV 16 and HPV 58 in 1 case. In Pakistan, 2 cases (1.43%) of HPV 16 were detected. Low-risk types include HPV 11 present in 2 cases from China, while HPV 6 was detected in 1 case each from both the countries.Conclusion
A low prevalence of HPV was found in China and Pakistan; high-risk and low-risk HPV were detectable in tonsillar tissue from both countries. Age stratification (< 5 years, 5–14 years, 15–25 years, > 25 years) suggest that sexual and non-sexual transmission of the virus can occur. The difference in the genotype distribution geographically within China and with Pakistan was observed in the case of HPV 58. The most common type in both the countries was HPV 16. 相似文献73.
Effective Enzyme Coimmobilization and Synergistic Catalysis on Hierarchically Porous Inorganic/Organic Hybrid Microbeads Fabricated Via Droplet‐Based Microfluidics 下载免费PDF全文
In this paper, a novel and robust droplet‐based microfluidic method to fabricate poly(ε‐caprolactone)/silica (PCL/SiO2) hybrid microbeads with hierarchically porous architecture is described and their performance as multienzyme carriers for cascade catalysis is further investigated in detail. In addition to the precise control on size and monodispersity of PCL/SiO2 microbeads enabled by the microfluidic method, the presence of ammonia as a catalyst for the hydrolysis and condensation of tetraethylorthosilicate makes it possible to manipulate the competition between sol–gel process and solvent extraction and thus adjust the surface porosity of hybrid microbeads, which eliminates the use of porogens/templates and also the complicated post‐treatment. Isothiocyanate‐immunoglobulin G/cyanine 3‐bovine serum albumin (FITC‐IgG/Cy3‐BSA) and superoxide dismutase/chloramphenicol acetyltransferase (SOD/CAT) are coimmobilized, respectively onto hierarchically porous PCL/SiO2 hybrid microbeads via either physical adsorption or covalent binding. Fluorescence intensity of coimmobilized FITC‐IgG/Cy3‐BSA proves that the proteins/enzymes immobilization amount via covalent binding is much higher than physical adsorption. The enhanced enzymatic activity, total antioxidant capacity, and reusability assay reveal that coimmobilized SOD/CAT exhibits better performance compared with the mono‐immobilized ones, mainly due to their mutual synergistic effect. The excellent results achieved in the work indicate that hierarchically porous PCL/SiO2 hybrid microbeads are very promising carriers for multienzymatic catalysis. 相似文献
74.
75.
Jingjing Jiang Hongling Wen Miaomiao Chi Ying Liu Jingxue Liu Zhankui Cao Li Zhao Yanyan Song Na Liu Lianli Chi Zhiyu Wang 《Virus genes》2018,54(3):333-342
Human parainfluenza virus type 3 (hPIV3) is an important respiratory pathogen that causes the majority of viral pneumonia of infants and young children. hPIV3 can infect host cells through the synergistic action of hemagglutinin-neuraminidase (HN) protein and the homotypic fusion (F) protein on the viral surface. HN protein plays a variety of roles during the virus invasion process, such as promoting viral particles to bind to receptors, cleaving sialic acid, and activating the F protein. Crystal structure research shows that HN tetramer adopted a “heads-down” conformation, at least two heads dimmer on flank of the four-helix bundle stalk, which forms a symmetrical interaction interface. The stalk region determines interactions and activation of F protein in specificity, and the heads in down position statically shield these residues. In order to make further research on the function of these amino acids at the hPIV3 HN stalk/head interface, fifteen mutations (8 sites from stalk and 7 sites from head) were engineered into this interface by site-directed mutagenesis in this study. Alanine substitution in this region of hPIV3 HN had various effects on cell fusion promotion, receptor binding, and neuraminidase activity. Besides, L151A also affected surface protein expression efficiency. Moreover, I112A, D120A, and R122A mutations of the stalk region that were masked by global head in down position had influence on the interaction between F and HN proteins. 相似文献
76.
Pengkai Cao Yingzhen Niu Chang Liu Xiaomeng Wang Guman Duan Qinghua Mu Xintong Luo Fei Wang 《The Knee》2018,25(1):59-65
Background
Tibial tuberosity–trochlear groove distance(TT-TG) is a measurement to assist in the diagnosis and treatment of patellar instability, however it still has some limitations. Our study was to modify the accepted measurement method and seek a more reliable and standardized method.Methods
The data of 65 healthy controls and 49 patients with bilateral patellar instability from 2010 to 2016 were collected and analyzed by CT. The TT-TG, tibial maximal mediolateral axis (MML), and their ratio [i.e., the modified-TT-TG (M-TT-TG)] were compared between the two groups.Results
The MML (71.9 ± 12.0 vs. 71.3 ± 10.9) was not significantly different between the two groups (P > 0.05). However, the TT-TG(18.1 ± 6.0 vs. 13.1 ± 2.9) and M-TT-TG (0.25 ± 0.08 vs. 0.19 ± 0.04) were significantly different between the two groups (P < 0.05). A TT-TG of > 15 mm was found in 24.5% of healthy controls and 71.5% of patients. The healthy controls with a TT-TG of > 15 mm were compared with the patients; although no significant difference was found in the TT-TG (16.8 ± 1.5 vs. 18.1 ± 6.0), the healthy controls had a significantly larger MML (76.9 ± 12.7 vs. 71.9 ± 10.9) and significantly smaller M-TT-TG (0.22 ± 0.04 vs. 0.25 ± 0.08). A total of 53.1% of patients but only 6.9% of healthy controls had an M-TT-TG of > 0.25.Conclusion
The M-TT-TG is a more reliable and standardized way to measure the effect of the TT-TG with the goal of reducing the false-positive rate associated with the standard measurement technique. The normal M-TT-TG ranges from 0.11 to 0.25, with an M-TT-TG of > 0.25 being associated with patellofemoral malalignment.Level of evidence
III. 相似文献77.
Yi-Kun Zhou Xiao-Chun Yang Yang Cao Heng Su Li Liu Zhi Liang Yun Zheng 《BMC medical genetics》2018,19(1):214
Background
Trichothiodystrophy nonphotosensitive 1 (TTDN1) is a disease with mental retardation, brittle hair. Some cases of the diseases are caused by mutations of the MPLKIP gene.Methods
We carefully identified the clinic characteristics, the sulfur level and pattern of the hair shafts of a female patient of with the symptom of hypergonadotropic hypogonadism, and of her parents and brother whose are healthy. We also collected the blood sample of the patient and performed the exon sequencing. One G insertion in MPLKIP was identified after analyzing the obtained exon sequencing profile. The G insertion sites in the patient, her parents and brother, were verified using Sanger sequencing. The G insertion in MPLKIP were compared to the dbSNP.Results
The female patient of TTDN1 carries a homozygous G insertion (rs747470385) in the MPLKIP gene. The parents and brother of the patient are heterozygous carriers of the same mutation, but are healthy. The hair shafts of the patient had a tiger-tail pattern with relatively low sulfur levels. To the best of our knowledge, this is the first report that autosomal recessive inheritance of the G insertion in the MPLKIP gene results in TTDN1.Conclusion
Our results indicate that the homozygotic G insertion in MPLKIP results in the TTDN1 with hypergonadotropic hypogonadism, while heterozygous carriers of the same mutation have no symptoms and healthy. These results provide novel insights into the association of mutations in MPLKIP and TTDN1 with hypergonadotropic hypogonadism.78.
79.
Time-average albumin predicts the long-term prognosis of IgA nephropathy patients achieved remission
Yuan Yanhong Wang Qin Zhang Minfang Cao Liou Che Xiajing Tian Lei Qi Chaojun Shao Xinghua Ni Zhaohui Mou Shan. 《中华肾脏病杂志》2015,31(2):102-108
Objective To clarify the long-term renal prognosis and related risk factors of progression for IgA nephropathy (IgAN) patients who achieved remission under current therapy. To identify the target value of the serum albumin level for Chinese patients with IgAN. Methods The patients with biopsy-proven primary IgAN in Nephrology Department of Renji Hospital in Shanghai were studied.The survival of renal and the relationships between clinical parameters and renal outcome were assessed. Results A total of 369 patients between Jan 2005 and Dec 2010 were included with a median follow-up time of 49.0 (38.0-65.8) months. All the subjects had achieved a complete remission (CR) or partial remission (PR) following six months’ therapy after diagnosis. Progressive renal disease had occurred in 61 cases at the end of follow-up. Three variables had a significant independent effect on renal outcome in patients achieving remission under current therapy regimen for IgAN, including time-average serum creatinine (TA-Scr) [HR(95%CI): 1.03(1.01-1.04)], time-average serum albumin (TA-Alb) [HR(95%CI): 0.83 (0.69-0.99)], and eGFR ratio within one year [HR(95%CI): 0.00(0.00-0.01)]. By multivariate Cox analyses, each 1 g/L drop of TA-Alb was related with 17.2% increase in the risk of renal progression. The ROC curve indicated that combination of serum albumin at baseline and during a long-term had a more significant value in prediction of renal outcome than independent predictor alone. By Kaplan-Meier analyses, patients with TA-Alb﹤38 g/L had a 10.4 fold sincreased risk of progressive disease compared with that of TA-Alb﹥38 g/L. Conclusions IgAN patients with lower eGFR ratio, higher TA-Scr and lower TA-Alb would progress to ESRD more quickly, and serum albumin during follow-up is important for predicting IgAN progression. 相似文献
80.
Philipp G. Smann Juan Eugenio Iglesias Boris Gutman Dominik Grotegerd Ramona Leenings Claas Flint Udo Dannlowski Emily K. Clarke-Rubright Rajendra A. Morey Theo G.M. van Erp Christopher D. Whelan Laura K. M. Han Laura S. van Velzen Bo Cao Jean C. Augustinack Paul M. Thompson Neda Jahanshad Lianne Schmaal 《Human brain mapping》2022,43(1):207-233
Structural hippocampal abnormalities are common in many neurological and psychiatric disorders, and variation in hippocampal measures is related to cognitive performance and other complex phenotypes such as stress sensitivity. Hippocampal subregions are increasingly studied, as automated algorithms have become available for mapping and volume quantification. In the context of the Enhancing Neuro Imaging Genetics through Meta Analysis Consortium, several Disease Working Groups are using the FreeSurfer software to analyze hippocampal subregion (subfield) volumes in patients with neurological and psychiatric conditions along with data from matched controls. In this overview, we explain the algorithm's principles, summarize measurement reliability studies, and demonstrate two additional aspects (subfield autocorrelation and volume/reliability correlation) with illustrative data. We then explain the rationale for a standardized hippocampal subfield segmentation quality control (QC) procedure for improved pipeline harmonization. To guide researchers to make optimal use of the algorithm, we discuss how global size and age effects can be modeled, how QC steps can be incorporated and how subfields may be aggregated into composite volumes. This discussion is based on a synopsis of 162 published neuroimaging studies (01/2013–12/2019) that applied the FreeSurfer hippocampal subfield segmentation in a broad range of domains including cognition and healthy aging, brain development and neurodegeneration, affective disorders, psychosis, stress regulation, neurotoxicity, epilepsy, inflammatory disease, childhood adversity and posttraumatic stress disorder, and candidate and whole genome (epi-)genetics. Finally, we highlight points where FreeSurfer-based hippocampal subfield studies may be optimized. 相似文献