Genes in the serotonin pathway are associated with bipolar affective disorder in a Han Chinese population

Serotonin plays an important role in mood regulation, but the involvement of serotonin pathway genes in the development of bipolar I disorder (BP-I), a mood disorder, is not clear. We selected 21 singlenucleotide polymorphisms (SNPs) within the HTR2A gene, 8 within the SLC6A4 gene and 23 within the TPH2 gene for genotyping using the GoldenGate genotyping assay. A total of 375 patients with BP-I and 475 normal controls were recruited. Two out of 21 SNPs (rs1475196 and rs9567747) in the HTR2A gene and 1/23 SNPs (rs17110566) in the TPH2 gene were significantly associated with BP-I, both genotype-wise and allele-wise. Furthermore, a specific haplotype in the HTR2A gene showed a significant association with BP-I. Our results indicate that the HTR2A and TPH2 genes in the serotonin pathway play important roles in susceptibility to BP-I.     

Multi-label classification of retinal lesions in diabetic retinopathy for automatic analysis of fundus fluorescein angiography based on deep learning

Graefe's Archive for Clinical and Experimental Ophthalmology - To automatically detect and classify the lesions of diabetic retinopathy (DR) in fundus fluorescein angiography (FFA) images using...     

A rapid method for PCR detection of bovine materials in animal feedstuffs

Rapid identification of bovine materials in animal feedstuffs is essential for effective control of a potential source of bovine spongiform encephalopathy. We have developed a rapid method for the detection of the presence of bovine materials in animal feeds. Animal feed samples were prepared by a Chelex-100 treatment method, then subjected to polymerase chain reaction (PCR) detection. The assay can be completed in 2 h including 30 min for sample preparation, 35-65 min for PCR cycling and 30 min for gel electrophoresis. This method is not only rapid, simple and consistent, but also avoids a hazardous waste disposal issue associated with a previously described guanidine thiocyanate (GuSCN) extraction-PCR method.     

慢性乙型肝炎患者血清角蛋白18裂解片段及其应用研究进展

近年来,血清角蛋白18及其裂解片段在肝病诊断方面的应用潜力引起了国内外研究者的强烈兴趣,但其在慢性乙型肝炎方面的应用研究较少。该文总结和探讨了循环血角蛋白18裂解片段M30及M65水平变化在慢性乙型肝炎中的意义以及应用前景。     

远红外线消毒柜对碗消毒效果的检测

为了解远红外线加热的消毒柜对餐具的消毒效果,用碗进行了试验观察。结果,加热至125℃作用10min,对碗上细菌繁殖体的杀灭率达90.0％以上,只能杀灭枯草杆菌黑色变种芽胞42.56％;装30只碗时,作用10min对自然菌的杀灭率可达90.5％,装40只碗时仅能杀灭64.9％。说明用该柜消毒餐具时装量不宜太多。     

社会因素与护理人员静脉穿刺成功率的关系

通过采用自身对照法，对１４名在岗护士两种心态下（正常心态与有社会因素影响）对病人手、足部位浅表静脉穿刺一次成功率进行比较。结果经Ｘ２检验，各单项比较无差异（ｐ＞０．０５），终值比较有显著差异（ｐ＜０．０５）。说明社会因素对静脉穿刺成功率有影响。