萘普生对大鼠着床期宫内PGF_(2α)含量及宫内节育器抗生育作用的影响

本文测定了对照组及两个萘普生(前列腺素合成酶抑制剂)处理组大鼠着床期宫内PGF2α的含量。结果表明,对照组、萘普生低剂量组(2mg/kg体重)和高剂量组(20mg/kg体重)的PGF2α含量分别为6.71±0.93、2.19±0.34和1.01±0.18ng/100g组织(三组间P<0.01)。同时,还观察到在本实验条件下,萘普生无明显干扰节育器抗生育作用,药物本身亦未呈现显著抗生育活性。     

Influence of Chemical Oxygen Demand Concentrations on Anaerobic Ammonium Oxidation by Granular Sludge From EGSB Reactor

INTRODUCTION The phenomenon of anaerobic ammonium oxidation was originally discovered in a denitrifying fluidized-bed reactor treating effluent from a methanogenic reactor in the 1990s[1]. Anaerobic ammonium oxidation (ANAMMOX) process is a strictly anaerobic denitrification process, in which ANAMMOX autotrophic bacteria directly oxidize ammonium to dinitrogen gas using nitrite as electron acceptor. In recent years, the anammox process has received great attention and lots of researc…     

PCR-SSP法HLA-A、B基因分型与血清学分型的比较

目的比较聚合酶链反应-序列特异性引物(PCR-SSP)进行HLA-Ⅰ类A、B抗原位点分型的准确性,并探讨血清学分型错误发生的原因。方法用PCR-SSP以及单克隆抗体血清学分型技术对HLA-A、B分型并比较。结果34例样本PCR-SSP基因分型无假阳性和假阴性出现。PCR-SSP法与血清学比较,血清学检出错误或漏检率分别为HLA-A位点23.5%,B位点26.5%。血清学发生错误或易混淆的抗原有:A2和A68、A32和A33,B5、B60和61。结论PCR-SSP法进行HLA-A、B抗原等位基因分型具有分辨率高、特异性强、重复性好、实验过程简捷快速、分型结果较血清学更加准确可靠的优点。     

特异性神经元抗体对神经系统副肿瘤综合征筛检价值的研究

目的　探讨特异性神经元抗体 (尤其是抗 Hu抗体 )对国内人群神经系统副肿瘤综合征 (paraneoplasticsyndromesofnervoussystem ,PNSNS)筛检诊断的特异性和灵敏性。方法　采用免疫组织化学ABC法和Westernblot对 2 0例PNSNS和 12 0例非PNSNS患者的血清进行检测 ,并采用流行病学筛检公式计算出特异度和灵敏度。结果　PNSNS组和非PNSNS组ABC法阳性结果差异有统计学意义 (χ2 =33 4 5 ,P <0 0 1) ,特异性神经元抗体筛检诊断PNSNS的灵敏度为 95 % ,特异度为 72 5 % ;PNSNS组和非PNSNS组Westernblot法阳性率差异有统计学意义 (χ2 =117 12 ,P <0 0 1) ,抗 Hu抗体筛检诊断的PNSNS的灵敏度为 95 % ,特异度为 98 3%。结论　血清特异性神经元抗体 (尤其是抗 Hu抗体 )是国内人群中PNSNS患者较敏感和特异性的血清标志物 ,在神经元的免疫损伤中具有重要作用     

一种FⅨ基因多态性在广西人群中的检出

目的研究广西人群ＦⅨ（凝血因子Ⅸ）基因多态性。方法利用聚合酶链反应（ＰＣＲ）和限制性内切酶Ｔｒｕ９Ｉ（同功酶即ＭｓｅＩ）酶解技术，对１２０名志愿者进行研究。结果首次在广西人群中发现ＦⅨ基因５′侧翼６９８位核苷酸存在Ｔｒｕ９Ｉ多态性，该多态性由８３ｂｐ／５８ｂｐ＋２５ｂｐ组成，二者的基因频率在受检的１８０个ＦⅨ基因中，分别为０．６２２和０．３７８，女性杂合子频率为０．４７３。结论该发现有助于血友病乙携带者的检测及产前基因诊断。     

齐墩果酸免疫治疗恶性肿瘤病人的临床Ⅱ期研究

前瞻性双盲法临床研究,可评价的恶性肿瘤病人28例,口服齐墩果酸40mg,每日3次,1个月后测定吞噬细胞百分比,由54.1％提高至67.42％。吞噬指数由0.69提高至0.81。迟发超敏反应1:1000浓度转阳性率52.6％(10/19)。E-玫瑰花结试验在本组试验前后无显著变化。同期服用安慰剂的9例对照病人则无变化。治疗后齐墩果酸对骨髓、肝、肾功能均无影响,故可做为比较理想的生物反应调节剂应用于临床,以促进病人的免疫功能。     

人胎盘微绒毛膜的制备及其转铁蛋白受体研究

作者分别用差速离心法及蔗糖梯度离心法制备胎盘微绒毛膜(PMM),用受体放射分析法研究了PMM的转铁蛋白受体(TfB),结果表明:两种方法制备的PMM均可用于受体分析,但差速离心法更为简便。测定60例产妇PMM的TfR,其数目为3.53±1.98×10~(12)个位点/mg膜蛋白,最大结合容量为6.33±4.21×10~(-12)mol/mg膜蛋白,Kd值为4.95±3.39×10~(-9)mol/L。受体结合反应体系最适实验条件为,膜蛋白浓度每管50μg,标记物浓度每管50 000cpm,孵育30分钟,B/F分离的聚乙二醇浓度为12％(W/V)。对TfR的特性研究表明:TfR与转铁蛋白的结合具有高度亲和力、高度特异性及可饱和性。     

Efficacy of Lung Cancer Screening; Comparison of Results from a Case-Control Study and a Survival Analysis

A case-control study to evaluate the efficacy of lung cancer screening conducted by us showed that lung cancer screening may reduce the mortality of the disease up to 28%. Assuming this efficacy is unbiased, and that the screening rate is 51.6%, which was observed in the control group in the above study, the number of lung cancer deaths prevented by screening in the study period was calculated to be 47 for males and females combined. In the same study population, screen-detected lung cancer patients (N = 207) in the same study period were followed and the 7-year survival rate (46.9%) was compared to the 5-year survival rate (11.3%) obtained by the Osaka Cancer Registry, in which screen-detected lung cancer patients were only 1.8%. The number of lung cancer deaths prevented by screening, estimated by the difference in the above two survival rates, was 74 (95% confidence interval; 55–93). The number of lung cancer deaths prevented by screening estimated from the case-control study was significantly lower than that estimated from the survival analysis. This indicates that the efficacy of lung cancer screening estimated by the case-control study was within the range that could be explained by the actual long-term survivors among the screen-detected patients in the study population.     

肝病患者肝组织中丙型肝炎病毒感染的初步研究

采用直接酶标记法对174例慢性肝炎、重症肝炎和肝癌组织中的HCV抗原进行了检测,HCV 抗原的检出率分别为10.71％、8.10％和2.68％。表明在我国慢性和重症肝病中存在HCV的感染。通过对HCV与HBV 感染关系的研究,发现肝内HBV 标志阳性和阴性者HCV抗原的检出率无明显差异,说明HCV感染与HBV感染之间似无明显关系。