Peripheral blood leukocyte telomere length is associated with age but not renal function: A cross-sectional follow-up study

Objectives

We aimed to evaluate the relationship between baseline renal function and changes in telomere length in Han Chinese.

Methods

The telomere restriction fragment (TRF) length of leukocytes in the peripheral blood was measured in healthy volunteers recruited in 2014. The estimated glomerular filtration rate (eGFR) was calculated based on serum creatinine (Scr) and serum cystatin C (CysC)-eGFRcys and eGFRScr-cys through the Cockcroft-Gault formula (eGFRC-G) or the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI / eGFRCKD-EPI) equation. The correlation between telomere length changes over time and renal function was analyzed.

Results

Leukocyte TRF lengths were negatively correlated to age (r = -0.393, p < 0.001) and serum CysC (r = -0.180, p < 0.01), while positively associated with eGFRCKD-EPI, eGFRC-G, eGFRcys, and eGFRScr-cys (r = 0.182, 0.122, 0.290, and 0.254 respectively, p < 0.01). The 3-year change of telomere length was 46 bp/years. When adjusted for age, the associations between telomere length changes and baseline, subsequent TRF lengths, and serum CysC were no longer present. No association was observed between TRF length changes and renal function.

Conclusion

The rate of telomere length changes was affected by age and baseline telomere length. The telomere length changes might be important markers for aging.

     

不同代数人牙周膜细胞成骨特征稳定性的实验研究

目的：观察培养代数牙周膜细胞的成骨细胞表型特征的影响。方法：利用4、6、8、10代细胞进行以形态学、细胞生长曲线、碱性磷酸酶（ALP）活性测定以及矿化能力和面积的观察。结果：在细胞形态和超微结构、细胞生长曲线、碱性磷酸酶活性和矿化能力方面，第4、6、8、10代无明显差别；10代以后的细胞各方面指标减弱，提示细胞进入衰老期。结论：利用10代以内牙周膜细胞进行实验或移植其结果可靠。     

Characterization of a transferrin-independent iron uptake system in rat primary cultured cortical cells

Multiple transferrin-independent iron uptake (Tf-IU) systems are known to be involved in the internalization of free iron salt in various mammalian cells. However, the characteristics of a Tf-IU system in cortical cells are not clear. In this study, we characterized the Tf-IU systems in both cortical and glial predominant (GP) cells from rat cerebral cortex to investigate the mechanism by which iron (Fe) or aliminum (Al) accumulates in the cerebral cortex. The Tf-IU systems in both cells were temperature- and Ca2*-dependent. The upregulation of Tf-IU system in GP cells by Fe- or Al-nitrilotriacetate (Fe- or AI-NTA) was 2- or 4-fold higher than that of cortical cells. Chemiluminescence analysis specific for O2- and 1O2 also showed that the production of active oxygen species in Fe-loading GP cells was twice higher than that of Fe-loading cortical cells. These results suggest the existence of Tf-IU systems in both cortical and GP cells similar to those of HeLa cells whose Tf-IU is metal- and Ca-2+ dependent, and that the glial cells contribute to metal accumulation in cerebral cortex. The present study may help to explain the pathogenesis of neurodegenerative disorders caused by inducing oxidative stress.     

家兔创伤性面神经损伤的组织病理学研究

为了建立各种面神经损伤的实验动物模型，对６８只家兔１３６侧面神经颊面干分别造成暴露、牵拉、挤压、压榨及切断后吻合与不吻合６种损伤，于伤后不同时间取材，切片行Ｂｏｄｉａｎ及Ｗｅｉｌ染色，光镜下观察神经轴突、髓鞘、Ｓｃｈｗａｎｎ细胞及神经纤维周围结缔组织的变化。结果表明，暴露可造成神经Ⅰ度损伤；挤压、牵拉、压榨可造成Ⅱ～Ⅲ度损伤；神经断裂后不吻合者预后明显较吻合者差。神经受损后的病理改变，在中、重度损伤与神经电图变化相一致，而轻度损伤及严重损伤后期两者可能不一致。伤后１５天为临床评价面神经功能恢复的最佳时间。神经受损面积对髓鞘病变影响明显，而损伤性质及程度对轴突病变影响明显。该动物模型的建立为深入进行面神经损伤及修复的研究提供了条件。     

Role of surgery in the treatment of patients with high-risk neuroblastoma who have a poor response to induction chemotherapy

Background

In instances of high-risk neuroblastoma that do not show a clinical response to induction therapy, whether it is worth performing surgical resection or not and whether gross total resection (GTR) is more important than subtotal resection (STR) remain controversial.

Methods

We retrospectively analyzed the data of patients with stage 4 neuroblastoma aged 18 months or older at diagnosis. Primary tumor volumes were measured both at diagnosis and at the first tumor response evaluation (after 6 cycles of induction chemotherapy). If the tumor volume at the first response evaluation was > 50% of the initial tumor volume, the patient was categorized as a poor responder. Otherwise, the patient was categorized as a good responder. Only poor responders were included. Patients were evaluated for event-free survival (EFS), overall survival (OS), and complications of surgery based on extent of surgical intervention.

Results

Sixty-five patients were included in this study. The 41 patients who underwent surgical intervention had a higher 3-year OS than the 24 patients who had a biopsy only (55.4% ± 8.1% vs. 31.3% ± 10.2%, P = 0.02). However, there was limited improvement in 3-year EFS following surgical intervention. Three-year EFS rates of BX group (biopsy only) and OP group (surgical resection) were 24.2% ± 9.3% and 37.7% ± 7.9%, respectively (P = 0.063). The extent of resection had no impact on 3-year OS (P = 0.631) and 3-year EFS (P = 0.796). Patients in the GTR group trended to have more severe surgical complications than patients in the STR group (P = 0.105).

Conclusions

For high-risk neuroblastomas that do not show a clinical response to induction therapy, surgical resection is important in predicting outcome, but the extent of resection is not.     

短板理论促进护理管理持续改善的实践

目的探讨运用短板理论进行护理管理体系重建和流程再造以达到促进护理管理持续改善的效果。方法运用精细化管理的ORTCC理论模型寻找医院护理管理体系中的短板,改善"目标、规则、训练、考核、文化"5个系统中存在系统化和细化的问题,重建医院护理管理体系,落实标准化护理工作流程和各护理岗位规范化工作程序,逐步实施同质化护理服务。结果管理前(2012年)和管理后(2013年)医院护理质量与安全管理年终考核平均分分别为89.40、93.10分,患者对护理工作满意率分别为88.74%、92.25%;护士对护理工作满意率分别为83.27%、91.12%。结论护理管理体系的最短木板是整体绩效的决定因素,补齐短板是提升整体绩效的关键要素,短板的存在和不断变化要求改善绩效必须是持续的,短板的加长赋予整体绩效改善以必然。     

先天性二叶主动脉瓣畸形27例临床分析

27例住院治疗的先天性二叶主动脉瓣(CBAV)临床分析,结果显示:合并心功能不全21例(78%),合并感染性心内膜炎(IE)12例(44%),与其它心血管畸形并存7例(26%).IE为CBAV的主要死亡原因.本文认为,并发IE或存在明显血流动力学障碍时外科换瓣术为CBAV治疗的根本措施,结果还提示,CBAV一旦心功能失代偿发展迅速,准确掌握外科治疗指征及尽早手术至关重要.