Effect of Wuzi Yanzong Pills on Sertoli cells and blood–testis barrier in heat-stressed rats based on Akt signalling pathway

The blood–testis barrier (BTB) of Sertoli cells (SCs) is an important biological barrier that maintains spermatogenesis and provides a favourable microenvironment for spermatogenesis. However, heat stress can directly damage the BTB structural proteins of testicular SCs, leading to dyszoospermia. Wuzi Yanzong Pills (WYP) is a traditional Chinese medicine formula used to treat male reproductive diseases. However, whether WYP could ameliorate heat stress injury in primary SCs extracted from rat testes and BTB proteins remains unknown. Here, treatment with WYP (low, medium and high dose) increased the SC viability and the proliferation of cell antigen Ki67 significantly. Additionally, it promoted SC maturation, which presented in the form of increased androgen receptors (ARs) and decreased cytokeratin 18 (CK-18) in three WYP dose groups. WYP upregulated BTB proteins such as zonula occludens 1 (ZO-1) and occludin across all WYP groups and decreased phosphorylated Akt (p-Akt) in the middle and high-dose groups; however, ZO-1 and occludin recovery were reduced with the presence of Akt inhibitor in WYP groups. WYP improved SC viability and proliferation, and ameliorated dedifferentiation and BTB-proteins damaged by heat stress via Akt signalling. The findings present theoretical support for the effects of WYP in the management of dyszoospermia and male infertility.     

Development a nomogram to predict fertilisation rate of infertile males with borderline semen by using semen parameters combined with AMH and INHB

The sperm quality of some males is in a critical state, making it hard for clinicians to choose the suitable fertilisation methods. This study aimed to develop an intelligent nomogram for predicting fertilisation rate of infertile males with borderline semen. 160 males underwent in vitro fertilisation (IVF), 58 of whom received rescue ICSI (R-ICSI) due to fertilisation failure (fertilisation rate of IVF ≤30%). A least absolute shrinkage and selection operator (LASSO) regression analysis identified sperm concentration, progressively motile spermatozoa (PMS), seminal plasma anti-Müllerian hormone (spAMH), seminal plasma inhibin (spINHB), serum AMH (serAMH) and serum INHB (serINHB) as significant predictors. The nomogram was plotted by multivariable logistic regression. This nomogram-illustrated model showed good discrimination, calibration and clinical value. The area under the receiver operating characteristic curve (AUC) of the nomogram was 0.762 (p < .001). Calibration curve and Hosmer–Lemeshow test (p = .5261) showed good consistency between the predictions of the nomogram and the actual observations, and decision curve analysis showed that the nomogram was clinically useful. This nomogram may be useful in predicting fertilisation rate, mainly focused on new biomarkers, INHB and AMH. It could assist clinicians and laboratory technicians select appropriate fertilisation methods (IVF or ICSI) for male patients with borderline semen.     

Early bioprosthesis failure in mitral valve replacement

The causes of early bioprosthesis failure include infective endocarditis, pannus formation, and structural valve deterioration. We reported a patient who suffered from early mitral bioprosthesis failure due to leaflets restricted by the subvalvular apparatus and early pannus formation. In patients with symptoms relapse and mitral regurgitation recurrence early after mitral valve replacement, early pannus formation needs to be anticipated, and surgical intervention should be performed if symptoms persist after medical treatment.     

基于儿科护士需求的人文关怀分层培训实践

目的探讨基于儿科护士需求分析的人文关怀分层培训的应用效果。方法采用访谈法了解不同层级儿科护士的人文关怀培训需求,根据培训需求对142名儿科护士实施人文关怀分层培训6个月,比较培训前及培训后各层级儿科护士关怀能力和培训满意度。结果培训后不同层级儿科护士关怀能力及对人文关怀培训满意度显著高于培训前(P<0.05,P<0.01)。结论采用基于需求的人文关怀分层培训有利于提高各层级儿科护士的人文关怀能力,提高培训满意度。     

中国住院癌症患者自杀意念发生率Meta分析

目的 了解中国住院癌症患者自杀意念发生率的总体情况,为住院癌症患者自杀行为预防及干预提供参考.方法 系统检索Web of Science、Cochrane Library、PubMed、EMbase、中国知网、中国生物医学文献服务系统、万方数据库、维普数据库自建库至2020年10月的相关文献,采用R4.0.2软件对数据进行Meta分析.结果 共纳入文献15篇,总样本量6328例.文献分析结果显示,我国住院癌症患者自杀意念发生率为21.6％[95％CI (15.9％,27.3％)].亚组分析结果显示,女性、使用多条目自杀测评工具、南方地区癌症患者自杀意念发生率较高.结论 我国住院癌症患者自杀意念发生率较高,综合医院应早期识别住院癌症患者的自杀意念,并及时对自杀意念及自杀行为采取预防及干预措施.     

减重术后患者饮食行为依从性量表的汉化及信效度检验

目的 汉化减重术后饮食行为依从性量表,并进行信效度评价.方法 通过翻译、回译,跨文化调适,形成减重术后饮食行为依从性量表中文版.便利抽取201例减重术后患者进行调查,分析量表信效度.结果 中文版减重术后饮食行为依从性量表总条目内容效度指数为0.94;条目水平的内容效度指数为0.820～1.000;量表探索性因子分析特征值＞1的公因子有2个,累积方差贡献率为64.128％,且各条目在其公因子上的负荷值为0.552～0.854;总量表的Cronbach's α系数为0.895,重测信度为0.835.结论 汉化版减重术后饮食行为依从性量表信效度良好,可应用于我国减重术后患者饮食行为依从性评估.     

人文关怀护理在合作医院的推进及成效

目的 探讨合作医院人文关怀护理推进策略及效果.方法 选派护理专家至合作医院进行驻点指导,建立人文关怀护理组织架构,制定人文关怀护理标准和制度,强化护理管理和服务理念,实施人文关怀培训、试点病房人文关怀护理,督导推进人文关怀护理规范化,全面推进合作医院人文关怀护理.结果 合作医院建立首批人文关怀试点病房12个,实施后护士关怀能力显著高于实施前(P＜0.01),患者对关怀护理满意度及护士工作满意度逐步提升.结论 人文关怀护理推进策略在合作医院的推进,促进了护士对患者的关怀、护理管理者对护士的关怀,可有效提高患者满意度、护士的关怀能力和工作满意度.