全文获取类型
收费全文 | 2083篇 |
免费 | 133篇 |
国内免费 | 8篇 |
专业分类
耳鼻咽喉 | 54篇 |
儿科学 | 25篇 |
妇产科学 | 52篇 |
基础医学 | 256篇 |
口腔科学 | 24篇 |
临床医学 | 224篇 |
内科学 | 425篇 |
皮肤病学 | 10篇 |
神经病学 | 138篇 |
特种医学 | 34篇 |
外科学 | 282篇 |
综合类 | 20篇 |
预防医学 | 237篇 |
眼科学 | 136篇 |
药学 | 217篇 |
中国医学 | 3篇 |
肿瘤学 | 87篇 |
出版年
2022年 | 19篇 |
2021年 | 54篇 |
2020年 | 18篇 |
2019年 | 44篇 |
2018年 | 45篇 |
2017年 | 29篇 |
2016年 | 47篇 |
2015年 | 36篇 |
2014年 | 61篇 |
2013年 | 106篇 |
2012年 | 100篇 |
2011年 | 131篇 |
2010年 | 67篇 |
2009年 | 71篇 |
2008年 | 107篇 |
2007年 | 125篇 |
2006年 | 118篇 |
2005年 | 119篇 |
2004年 | 101篇 |
2003年 | 117篇 |
2002年 | 105篇 |
2001年 | 22篇 |
2000年 | 20篇 |
1999年 | 22篇 |
1998年 | 19篇 |
1997年 | 34篇 |
1996年 | 27篇 |
1995年 | 18篇 |
1994年 | 23篇 |
1993年 | 21篇 |
1992年 | 15篇 |
1991年 | 13篇 |
1990年 | 16篇 |
1989年 | 14篇 |
1988年 | 10篇 |
1987年 | 13篇 |
1986年 | 18篇 |
1985年 | 10篇 |
1984年 | 21篇 |
1983年 | 10篇 |
1982年 | 14篇 |
1981年 | 20篇 |
1980年 | 20篇 |
1979年 | 15篇 |
1978年 | 14篇 |
1977年 | 15篇 |
1976年 | 13篇 |
1975年 | 28篇 |
1972年 | 9篇 |
1968年 | 9篇 |
排序方式: 共有2224条查询结果,搜索用时 15 毫秒
71.
72.
Roles of Glycoproteins and Oligosaccharides Found in Human Vaginal Fluid in Bacterial Adherence 下载免费PDF全文
Nithya Rajan Qing Cao Byron E. Anderson Denise L. Pruden Julia Sensibar James L. Duncan Anthony J. Schaeffer 《Infection and immunity》1999,67(10):5027-5032
Adherence of type 1-piliated Escherichia coli to carbohydrate structures of vaginal mucosa plays a major role in the pathogenesis of ascending urinary tract infections in women. Colonization of the vaginal introitus is influenced by interactions between pathogens, vaginal fluid, and vaginal epithelium. In this study, the type and amount of carbohydrates and glycoproteins present in vaginal fluid were determined. Free and protein-bound oligosaccharides in vaginal fluid specimens were analyzed by fluorophore-assisted carbohydrate electrophoresis (FACE) and high-pressure liquid chromatography (HPLC). Two-dimensional electrophoretic separations of vaginal fluid glycoproteins were performed together with bacterial overlay assays. The results of FACE showed that the majority of the oligosaccharides are in the free state and the bound oligosaccharides are undetectable. HPLC analysis of free sugars revealed glucose as the major sugar (3.3 +/- 0.3 mM), and the concentrations of mannose and glucosamine were 0.065 +/- 0.04 and 0.02 +/- 0.001 mM, respectively. Radiolabeled E. coli bound three vaginal fluid glycoproteins with the following molecular masses and pIs: 82 kDa and pI 5.5, 55 kDa and pI 4.5, and 55 kDa and pI 6.5. The binding was inhibited by mannose and by deglycosylation of the proteins prior to the overlay assay. One of these putative receptors was identified to be the heavy chain of secretory IgA (S-IgA). These data suggest that the free mannose in the fluid is less than that required to affect E. coli-epithelial cell binding interactions and that S-IgA may bind E. coli in the vaginal introitus. 相似文献
73.
74.
Hung Huynh Van Chanh Ngo Joseph Fargnoli Mark Ayers Khee Chee Soo Heng Nung Koong Choon Hua Thng Hock Soo Ong Alexander Chung Pierce Chow Pamela Pollock Sara Byron Evelyn Tran 《Clinical cancer research》2008,14(19):6146-6153
PURPOSE: Hepatocellular carcinoma (HCC) is the fifth most common primary neoplasm; surgery is the only curative option but 5-year survival rates are only 25% to 50%. Vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) are known to be involved in growth and neovascularization of HCC. Therefore, agents that target these pathways may be effective in the treatment of HCC. The aim of this study was to determine the antineoplastic activity of brivanib alaninate, a dual inhibitor of VEGF receptor (VEGFR) and FGF receptor (FGFR) signaling pathways. EXPERIMENTAL DESIGN: Six different s.c. patient-derived HCC xenografts were implanted into mice. Tumor growth was evaluated in mice treated with brivanib compared with control. The effects of brivanib on apoptosis and cell proliferation were evaluated by immunohistochemistry. The SK-HEP1 and HepG2 cells were used to investigate the effects of brivanib on the VEGFR-2 and FGFR-1 signaling pathways in vitro. Western blotting was used to determine changes in proteins in these xenografts and cell lines. RESULTS: Brivanib significantly suppressed tumor growth in five of six xenograft lines. Furthermore, brivanib-induced growth inhibition was associated with a decrease in phosphorylated VEGFR-2 at Tyr(1054/1059), increased apoptosis, reduced microvessel density, inhibition of cell proliferation, and down-regulation of cell cycle regulators. The levels of FGFR-1 and FGFR-2 expression in these xenograft lines were positively correlated with its sensitivity to brivanib-induced growth inhibition. In VEGF-stimulated and basic FGF stimulated SK-HEP1 cells, brivanib significantly inhibited VEGFR-2, FGFR-1, extracellular signal-regulated kinase 1/2, and Akt phosphorylation. CONCLUSION: This study provides a strong rationale for clinical investigation of brivanib in patients with HCC. 相似文献
75.
Byron E. Butterworth Catherine S. Sprankle Susan M. Goldsworthy Daniel M. Wilson Thomas L. Goldsworthy 《Molecular carcinogenesis》1994,9(1):24-32
Furan administered by gavage for 2 yr has been reported to induce hepatocellular carcinomas in male and female B6C3F1 mice and in male but not female F344 rats. Chronic exposure studies in our laboratory using bioassay conditions showed extensive hepatocellular toxicity and sustained increases in regenerative cell proliferation after 1,3, and 6 wk of treatment in male and female rats and male mice. Altered expression of growth-control genes associated with this hyperproliferative state may enhance the susceptibility of these genes to mutation or may provide a selective growth advantage to preneoplastic cells. Quantitative northern blot analysis of mRNA was used to examine the expression of the oncogenes myc, fos, and Ha-ras in the livers of animals treated with furan. In male rats, a single administration of 30 mg/kg furan produced necrosis and a subsequent wave of cell proliferation 48 h after treatment and induced transient peaks in the expression of myc, fos, and Ha-ras 6–24 h after treatment. In male rat liver from our cell proliferation studies, only a slight increase in myc expression was seen at the end of week 1 of treatment. However, beginning at week 3 and increasing at week 6, up to a 15-fold increase over control values was observed in the expression of myc in the treated animals. The only other notable increase in expression observed in any animals from the cell proliferation study was a threefold increase in myc at week 6 in treated female rats. The absence of an increase in Ha-ras expression in the male mouse liver suggests that the unique patternof Ha-ras mutations previously reported in furan-induced mouse liver tumors is not due to increased mutational susceptibility related to overexpression of this gene. The lack of sustained expression of myc, fos, and Ha-ras in rapidly proliferating liver suggests that continuous expression of these genes is not necessary to maintain increased rates of cell replication. The large increase in myc expression in male but not female rats suggests an adaptive change that may be related to the sex-specific incidence of furan-induced hepatocellular carcinomas in rats. © 1994 Wiley-Liss, Inc. 相似文献
76.
77.
78.
79.
Cryer BL 《Arthritis and rheumatism》2003,48(7):2074-5; author reply 2075-7
80.
Cryer B 《Current gastroenterology reports》2003,5(6):453-458
This article reviews the gastrointestinal manifestations of traditional nonsteroidal anti-inflammatory drugs (NSAIDs) and
the improved gastrointestinal safety profile of cyclooxygenase selective (COX)-2 inhibitors. By inhibiting the COX enzyme,
NSAIDs provide effective analgesia and suppress inflammation in a variety of conditions. Most NSAIDs (nonselective or traditional)
not only inhibit prostaglandins at sites of inflammation but also inhibit prostaglandins that have important normal functions
in other parts of the body. This may be harmful when normal gastrointestinal mucosal function is impaired and mucosal damage
occurs. Although such damage is often trivial and usually not symptomatic, gastrointestinal ulceration may produce pain and,
more ominously, lead to bleeding, perforation, or obstruction. A new approach to the gastrointestinal complications of NSAIDs
became feasible with the discovery of two isoforms of COX, COX-1 and COX-2, with COX-1 expressed mainly in the gastrointestinal
tract. The development of NSAIDs that preferentially inhibit COX-2 offers the promise of relieving pain and inflammation without
the side effects attendant to COX-1 blockade. In prospective studies evaluating gastrointestinal ulceration with COX-2-specific
NSAIDs, rates of endoscopic ulceration have been equivalent to those with placebo and much lower than those with nonselective
NSAIDs. In the recently released studies of gastrointestinal outcomes (perforated, painful, or bleeding ulcers), incidence
of clinically relevant ulceration with COX-2 NSAIDs is much lower than that of traditional NSAIDs. 相似文献