工频磁场对星形胶质细胞间隙连接通讯功能的影响

目的　探讨工频磁场 (PFMF)是否有促癌或协同促癌作用。方法　利用荧光光漂白后再恢复法观察漂白细胞荧光强度的恢复以判断经间隙连接的细胞间通讯 ,以相对荧光强度恢复速率(CFIRR)作为对细胞间隙连接通讯 (GJIC)作用的评价指标 ,研究不同磁场强度单独作用或协同佛波酯(TPA)对星形胶质细胞GJIC功能的影响。结果　 3ng/mlTPA作用 1h时CFIRR的中位数 (Md)值为4 .53 % /min ,空白对照组为 9.74% /min ,两组的差异有显著性 (H =1 2 .0 84,P <0 .0 0 5)。 0 .8或 1 .6mT磁场作用 2 4h时CFIRR的Md 分别 8.2 5、6 .68% /min ,与空白对照组比较 ,差异无显著性 (H =32 .61 7,P >0 .0 5)。 0 .8或 1 .6mT磁场作用 2 3h ,再与TPA共同作用 1h时CFIRR的Md 分别为 3 .32、2 .85% /min ,与TPA组比较 ,差异无显著性 (H =2 .589,P >0 .0 5)。结论　 0～ 1 .6mT的 50Hz磁场单独作用不能抑制星形胶质细胞GJIC功能 ;协同TPA ,不能增强TPA对星形胶质细胞GJIC的抑制作用 ;但是磁场对星形胶质细胞GJIC的抑制作用随着磁场强度增强呈递增趋势     

口外弓配合唇档远移磨牙的临床研究

目的:研究口外弓配合唇档远移磨牙的临床应用及其疗效。方法:选择15例恒牙早期上颌轻度牙性前突或轻、中度拥挤的安氏II类患者,应用口外弓配合唇档,通过X线头影测量观察其上颌磨牙及前牙治疗前后变化情况。结果:应用口外弓配合唇档推磨牙向远中3～6个月后,II类咬合关系恢复至安氏I类关系。上颌第一磨牙向远中平均移动4.14mm,上颌中切牙向唇侧移动0.66mm。结论:只要病例选择合适,方法运用得当,口外弓配合唇档就能获得较满意的远移磨牙的疗效。     

高分子染料的进展

高分子染料是一类本身固有颜色的高分子化合物，它兼有染料和高聚物的双重性质。因此既可作为色料也可作为高聚物而应用于许多领域。本文较详细阐述了聚合染料的种类，合成方法及应用情况。     

汉族、哈萨克族Graves病患者血清CoL IV、LN和HA测定分析

用放免法(单克隆抗体)测定汉、哈萨克族Graves病未经治疗患者66例及正常对照组55例血清中Ⅳ型胶原(CoLⅣ)、板层素(IN)和透明质酸(HA),结果Graves病患者血清CoLⅣ、LN浓度明显升高,HA浓度明显降低,与正常对照组相比均有显著性差异(分别P<0.001、P<0.05、P<0.05),汉族与哈萨克族患者之间、对照组之间相比均无显著性差异(P>0.05),它反映了Graves病患者体内基底膜合成和分解代谢紊乱。     

Active oxygen radicals produced by leukocytes of malignant lymphoma.

The electron spin resonance (ESR) spectra of active oxygen radicals produced during the respiratory burst of PMA-stimulated leukocytes and oxygen consumption are studied by ESR spin trapping and spin probe oxymetry for 31 times in 17 cases of malignant lymphoma. The results showed that the spectra produced from PMA-stimulated patients' leukocytes were predominantly spin adducts of DMPO-hydroxyl radicals (DMPO-OH). The decrease in oxygen consumption during respiratory burst suggested that respiratory burst function was inhibited. Kinetic observations of patient condition showed that respiratory burst and oxygen consumption were improved and even approached normal, when the patient was in remission or the condition was ameliorated. This paper is the first of its type and helps clarify the mechanism of malignant proliferation and metastasis.

     

蚌埠地区人群金葡菌带菌调查及药敏试验

本文对不同人群抽样调查1101人,结果鼻咽部金葡菌带菌者222人。其中医务人员和住院患者的金葡菌带菌率明显高于院外人群(P<0.001）。提示医院内存在交叉感染。对222株金葡菌测定药敏试验及β-内酰胺酶,结果β-内酰胺酶阳性率89.6％。产酶株全部对青霉素耐药,不产酶株对青霉素敏感率91.4％。产酶与否,对红霉素、氯霉素耐药严重。利福平、苯唑青霉素、丁胺卡那霉素对金葡菌有极高的抗菌活性。     

Alterations of G-protein Coupling Function in Phosphoinositide Signalling Pathways of Rat Hippocampus by Ischaemic Brain Injury

The activation of membrane-associated phospholipase C is rapidly and transiently induced in the central nervous system by a variety of stimuli. Ischaemic brain injury is one of the situations that leads to a dramatic increase in polyphosphoinositide (PPI) turnover. In this study, stimulation of PPI hydrolysis by glutamate (500 μM) was measured in hippocampal slices from rats up to 21 days after an ischaemic insult of 30 min. Ischaemia was induced using the four-vessel occlusion method. PPI hydrolysis elicited by glutamate was significantly increased in the slices prepared from ischaemic rats 24 h after reperfusion, the accumulation of inositol phosphates (InsP_s) and inositol 1,4,5-trisphosphate (InsP₃) was 614±74% ( n = 8) and 182±11% ( n = 9) of the basal level respectively. This potentiation was also observed 21 days after ischaemia. Hyper-responsiveness to glutamate was also accompanied by an increase in AIF⁻₄-stimulated formation of [³H]inositol phosphates. In addition, global ischaemia did not change either high-affinity [³H]glutamate binding in hippocampal membranes or the stimulation of PPI hydrolysis by carbachol or noradrenaline in hippocampal slices. The present results suggest that the increased responsiveness to glutamate is the result, at least in part, of functional changes at the G-protein level, and may contribute to the pathophysiology of ischaemic brain injury or to the regenerative phenomena that accompany ischaemic damage.     

鼻腔粘膜吸收庆大霉素功能的研究

用静脉内、肌肉内及鼻腔内三种给药途径给１０只狗按５ｍｇ／ｋｇ用４０ｍｇ／ｍｌ的庆大霉素，并使用双波长色谱扫描法测定０～１８０分钟血清庆大霉素的浓度，比较其生物利用度。发现鼻内给药的生物利用度为静脉注射的８０．５％，肌肉注时为４２％。鼻腔用药后１０～３０分钟血清浓度达到高峰，范围在３．７～６．６μｇ／ｍｌ，半衰期长达３小时左右，表明鼻粘膜对庆大霉素有良好的通透性，可使其迅速吸收入血，并能在血中长时间维持有效浓度。     

脾结核的临床特征

目的 探讨脾结核的临床特点、诊断和治疗。方法 回顾性分析经临床、手术及病理证实的11例脾结核的临床特征。结果 11例患者中，男7例，女4例，平均年龄29岁。6例术后证实，5例经内科保守治疗后证实。多数患者表现为发热、消瘦、血沉增快、贫血、脾肿大，B超发现脾脏肿大11例，其中8例脾内多发占位，3例为孤立性占位；7例行CT检查，6例发现脾脏多发性大小不等的结节状低密度灶，1例为单发类圆形低密度影，边缘不清，增强扫描病灶内部无强化；6例患者伴后腹膜、脾门区等淋巴结肿大，5例患者伴其它脏器结核。结论 不明原因发热、消瘦、血沉增快、贫血、脾肿大为脾结核的主要临床表现，腹部B超和腹部CT能对脾结核作出较准确的判断，脾结核患者经正规治疗可治愈。     

天麻化学成分研究(Ⅰ)

目的 研究天麻中的弱极性成分。方法 硅胶柱色谱结合制备薄层层析分离，光谱分析鉴定结果。结果 分离并鉴定了7个化合物。他们分别为：对差劲苄基乙基醚（I），β-谷甾醇（Ⅱ），棕榈酸（Ⅲ），2，2’-亚甲基-二（6-叔丁基-4-甲基苯酚）（Ⅳ），对羟苄基甲醚（Ⅴ），邻苯二甲酸二甲酯（Ⅵ）和对羟基苯甲醛（Ⅶ）。结论 化合物Ⅳ和Ⅵ为天麻中首次报道成分，其中，化合物Ⅳ为四取代苯的二聚体，为首次从天麻属及兰科植物中分离鉴定。