缺血性脑血管病患者颈动脉粥样硬化斑块与超敏-CRP关系

目的探讨脑梗死患者颈动脉粥样硬化性斑块与超敏C-反应蛋白（Hs-CRP）含量的关系。方法分别测定30名通过颈动脉超声检查发现有粥样硬化斑块的急性脑梗死（A组）、脑供血不足患者（B组）和无颈动脉粥样硬化斑块老年健康体检者（C组）血清中Hs-CRP含量。结果A组Hs-CRP含量明显高于B组和C组（P〈0.05）;而B组和C组之间无显著性差异。结论Hs-CRP与动脉硬化引起的急性脑梗死有关,Hs-CRP与稳定性颈动脉硬化性斑块患者的脑供血不足的症状无关。     

人胃癌SGC7901细胞膜钾离子通道的特性

目的　研究人胃癌细胞系 SGC790 1细胞膜钾离子通道特性 .方法　以人胃癌细胞系 SGC790 1为研究对象 ,采用穿孔膜片钳全细胞记录法 .结果　人的胃癌 SGC790 1细胞系的静息膜电位为 (- 32 .2± 2 .2 ) m V,在钳制电压 - 40 m V,阶跃电压在 - 80～ +80 m V,记录到一种跨膜电流 ,该跨膜电流具有电压依赖、外向整流特性 ,该电流在延迟 2 5 ms后最大激活 ,80 0 m s内不失活 ,翻转电位在 - 6 0～ - 40 m V,能被已知的钾通道的阻断剂 4- AP或 TEA阻断 .结论　在人胃癌SGC790 1细胞系细胞膜上存在延迟整流钾离子通道 .     

血清抑制素A在预测孕早期先兆流产孕妇妊娠结局中的应用

目的探讨抑制素A在预测孕早期先兆流产孕妇妊娠结局中的价值。方法选择孕6～13周稽留流产孕妇18例及B超检查示活胎的先兆流产孕妇27例（妊娠继续组17例和难免流产组10例）,同孕龄正常孕妇40例。采用ELISA法测定各组孕妇血清抑制素A水平。结果正常孕妇血清抑制素A在孕早期随孕龄增加而增加,孕10～11周达高峰,孕12～13周逐渐降低。难免流产组血清抑制素A水平明显低于妊娠继续组（P〈0.01）;当抑制素A〈0.3 MoM时可筛查出90%的难免流产,敏感性为90%,特异性为94.1%。结论孕早期血清抑制素A的检测有助于预测先兆流产孕妇不良妊娠结局,当血清抑制素A〈0.3 MoM时对预测难免流产有较高的敏感性和特异性。     

Predictors of post coronary artery bypass grafting atrial fibrillation

Objective: To investigate the incidence and relative risk factors of post coronary artery bypass grafting(post-CABG) atrial fibrillation (AF). Methods: 312 patients with CABG were reviewed and divided into an AF group and a non-AF group. Statistical analysis was used to compare the data between the two groups and screen for risk factors of post-CABG AF. Results: 103/312(33.01%) patients developed post-CABG AF. Univariate analysis showed that patients in AF group compared with those in non-AF group were more likely to have advanced age (≥ 70 years), early postoperative withdrawal of β-blockers, hypertension, left atrial enlargement ( ≥40 mm), a history of AF, prolonged p-wave duration ( ≥ 120 ms) and increased number of grafts (≥3). Multivariate logistic regression analysis showed that advanced age (≥70 years), early postoperative withdrawal of β-blockers, hypertension, left atrial enlargement (≥40 mm) and a history of AF were highly related to post-CABG AF. Conclusion: The incidence of AF in patients following CABG was 33.01% in this study. Advanced age, early postoperative withdrawal of β-blockers,hypertension, left atrial enlargement and a history of AF were independent risk factors of post-CABG AF.     

Expansion of CD3+CD8+PD1+ T lymphocytes and TCR repertoire diversity predict clinical responses to adoptive cell therapy in advanced gastric cancer

The adoptive cell therapy (ACT) and delivery of ex vivo activated cellular products, such as dendritic cells (DCs), NK cells, and T cells, have shown promise for the treatment of gastric cancer (GC). However, it is unknown which cells can improve patient survival. This study was focused on the antitumour activity of a subset of these cellular products and their relationships with clinical outcomes. Nineteen patients were enrolled at the Capital Medical University Cancer Center, Beijing Shijitan Hospital, from June 1, 2013, to May 30, 2016. CD8⁺PD1⁺ T-cell sorting was carried out using flow cytometry, and the T-cell receptor (TCR) repertoire during ex vivo expansion for 15 days was analyzed by next-generation sequencing. After 15 days of culture, the number of CD8⁺ T cells had increased significantly, and the number of CD4⁺ T cells had increased correspondingly. After ex vivo expansion, CD8⁺ T cells exhibited significantly enhanced expression of PD-1, LAG-3, and TIM-3 but not 4-1BB. Survival analysis showed that patients with a pro/pre value of CD8⁺PD-1⁺ T cells >2.4 had significantly favorable overall survival (OS) (median OS time, 248 days versus 96 days, P=0.02) and progression-free survival (PFS) (median PFS time, 183 days vs. 77 days, P=0.002). The sorted CD8⁺PD-1⁺ T cells displayed enhanced antitumor activity and increased IFN-γ secretion after coculture with autologous tumor cell lines. TCR repertoire diversity was decreased after ex vivo expansion, which decreased the Shannon index and increased the clonality value. The prognosis of patients was significantly improved and was associated with the extent of CD8⁺PD-1⁺ T-cell expansion. In summary, this study showed that after ex vivo expansion for 15 days, CD8⁺PD-1⁺ T cells could be identified as tumor-reactive cells in patients treated for GC. Changing TCR species can predict the extent of CD3⁺CD8⁺PD1⁺ T-cell growth and the effect of ACT treatment.     

军队鼠害综合防治技术规范的制定

通过查阅分析相关文献、现场调研、征求专家意见及会议审定研究制定军队标准《军队鼠害综合防治技术规范》。该标准规范了军队鼠害综合治理,为依法开展鼠害防治工作提供技术支持。     

肾透明细胞癌CYP4A11、Ki-67表达及与患者临床病理特征的关系

目的 研究细胞色素P4504A11(CYP4A11)及Ki-67在肾透明细胞癌中的表达及相关性,并探讨其与患者临床病理特征的关系.方法 采用免疫组织化学染色方法检测我院2010—2015年收治的行肾癌切除术并经病理诊断的151例肾透明细胞癌组织中CYP4A11及Ki-67的表达情况,分析CYP4A11及Ki-67的表达...     

基于网络药理学和多中心临床数据的清肺承气汤治疗腹腔感染所致急性呼吸窘迫综合征的作用机制研究

目的 采用网络药理学和多中心临床数据探讨清肺承气汤治疗腹腔感染所致急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)的作用机制.方法 通过中药系统药理学分析平台(Traditional Chinese Medicine Systems Pharmacology Dat...