Nd∶YAG激光周边虹膜切除术对视觉质量的早期影响

目的:评价Nd∶YAG激光周边虹膜切除术(LPI)对视觉质量的早期影响.方法:前瞻陛自身对照研究.利用双通道视觉质量分析系统检测2016年3-6月在北京协和医院眼科行LPI的患者在LPI前和1周后的客观散射指数(OSI)、调制传递函数截止频率(MTF cut-off)、斯特列尔比(SR)、客观对比度视力、平均客观散射指数(mean OSI)以及调节力.对LPI治疗前后各参数进行配对t检验和秩和检验分析.结果:共42例(52眼)患者接受LPI,年龄41～ 80岁,平均(63.5±8A)岁.LPI前与LPI治疗1周后视力、眼压比较差异无统计学意义;100％、20％和9％对比度视力在LPI前分别为0.73±0.37、0.49±0.25和0.29±0.13,LPI治疗1周后分别为0.77±0.30、0.54±0.26和0.32±0.15,LPI治疗前后差异无统计学意义;LPI前与LPI治疗1周后OSI、MTF cut off、SR、mean OSI以及调节力比较差异均无统计学意义.结论:Nd∶YAGLPI对早期视觉质量、调节力等均无明显影响,是预防性治疗原发性前房角关闭安全、有效的方法.     

Endoscopy screening effect on stage distributions of esophageal cancer: A cluster randomized cohort study in China

Efficacy of endoscopic screening for esophageal cancer is not sufficiently definitive and lacks randomized controlled trial evidence. The present study proved short‐term screening efficacy through describing and comparing disease stage distributions of intervention and control populations. Villages from Linzhou and Cixian were cluster randomly allocated to the intervention or to the control group and the target population of 52 729 and 43 068 individuals was 40‐69 years old, respectively, and the actual enrolled numbers were 18 316 and 21 178, respectively. TNM stage information and study‐defined stage information of esophageal cases from 2012 to 2016 were collected. Stage distributions were compared between the intervention and control groups in the total target population, as well as in the subgroup populations in terms of enrolment and before or after intervention. There were a total of 199 and 141 esophageal cancer cases in the intervention and control groups, respectively. For the target population, distributions of TNM stage were borderline significant between the two groups after intervention (P = .093). However, subgroup analysis of the enrolled population during the after‐intervention period had statistical significance for both TNM and study‐defined stage. Natural TNM stage distributions were approximately 32%, 41%, 24% and 3% for stages I to IV vs 71%, 19%, 7% and 3% in the intervention population. The natural study‐defined stage distributions from early, middle to advanced stages were approximately 18%, 49% and 33% vs 59%, 33% and 8%. Early‐stage esophageal cancer cases accounted for a higher proportion after endoscopy screening, and the efficacy in the target population depends on the intervention compliance.     

牛磺酸对糖尿病性白内障防治的实验研究

目的探讨牛磺酸对链脲佐菌素（ＳＴＺ）诱导的大鼠糖尿病性白内障的防治效果。方法１４０只ＳＤ大鼠随机分为６组,实验组通过口服、点眼、结膜下注射等途径补充牛磺酸,定期观察晶状体的变化,测定血糖及晶状体中山梨醇、超氧化物歧化酶（ＳＯＤ）、丙二醛（ＭＤＡ）含量并与对照组相比较。结果白内障时晶状体中山梨醇及ＭＤＡ含量显著增加,ＳＯＤ活力明显下降。各牛磺酸用药组晶状体中山梨醇含量无显著性改变,但ＳＯＤ活力明显增加,ＭＤＡ含量明显降低,白内障发生时间推迟,混浊程度减轻,全身应用牛磺酸还可以降低血糖。结论牛磺酸对ＳＴＺ诱导的糖尿病性白内障有一定的防治作用。     

复方苦参注射液对晚期消化道肿瘤患者血清白细胞介素2水平的影响

目的探讨复方苦参注射液对晚期消化道肿瘤患者血清白细胞介素2表达水平的变化及临床意义。方法用双抗体夹心法检测血清白细胞介素2的水平,然后比较实验组和对照组水平的差异。结果对照组治疗前后白细胞介素2水平,无显著差异(P>0.05)。实验组治疗前数据(14.505±1.071)pg/ml,治疗后数据(25.917±1.914)pg/ml,两者比较,治疗后均数明显大于治疗前(P<0.05),有显著差异;实验组和对照组比较(P<0.05),有显著差异。结论复方苦参注射液能够提高晚期消化道肿瘤患者血清白细胞介素2水平,提高患者免疫力,发挥抗肿瘤作用。     

经尿道钬激光膀胱肿瘤切除术对表浅层膀胱癌患者术中出血量及预后的影响

目的 探讨经尿道钬激光膀胱肿瘤切除术对表浅层膀胱癌患者术中出血量及预后的影响.方法 选取新乡医学院第三附属医院2013年9月至2016年1月收治的85例表浅层膀胱癌患者,根据手术术式不同分为对照组42例(给予经尿道膀胱肿瘤电切术治疗)与观察组43例(实施经尿道钬激光膀胱肿瘤切除术治疗),观察比较两组手术情况(膀胱冲洗时间、留置尿管时间、术中出血量)及手术创伤相关指标[胰岛素抵抗指数(HOMA-IR)、纤维蛋白原(FIB)、凝血酶原活动度(PTA)],并比较两组并发症发生率及复发率.结果 观察组术中出血量及留置尿管、膀胱冲洗时间均小于对照组,差异均有统计学意义(均P＜ 0.05);观察组HOMA-IR低于对照组,FIB及PTA均高于对照组,差异均有统计学意义(均P＜ 0.05);观察组并发症发生率为4.65％(2/43),低于对照组21.43％(9/42),差异有统计学意义(P＜0.05);观察组复发率为2.33％(1/43),低于对照组19.05％(8/42),差异有统计学意义(P＜0.05).结论 经尿道钬激光膀胱肿瘤切除术治疗表浅层膀胱癌,效果显著,可减少术中出血量,改善患者手术创伤相关指标值及预后.     

不同储存温度和时间对糖尿病患者尿白蛋白测定的影响

研究了不同保存时间和温度对糖尿病患者尿白蛋白排出的率测定的影响。４１例糖尿病患者１２小时尿样本分别储存于室温、４℃、－２０℃的条件下，于当天、１４、３０、６０天测定尿白蛋白的含量。结果显示在４℃储存时，尿白蛋白浓度在６０天时无明显改变。室温下储存６０天时有轻度的降低。－２０℃储存一个月后有明显下降，９例（３９．１％）患者ＵＡＥ从２０－２００μｇ／ｍｉｎ降至正常范围，而４℃条件下只有３例（１３．１￥     

Toll样受体4在脓毒症大鼠胰岛中的表达

目的 研究Toll样受体4(TLR4)在脓毒症大鼠胰岛中的表达变化及其对血糖的影响.方法 SPF级SD雄性大鼠腹腔内注射脂多糖(LPS)5 mg/kg,间隔3h注射第2次,制备脓毒症模型.大鼠被随机(随机数字法)分为正常对照组(n=5)、LPS组(n=5)、抗TLR4抗体组(n=5)和抗TLR4抗体+LPS组(n=5),分别采用RT-PCR法、Western-blot法和免疫组化法检测大鼠胰岛TLR4的表达.大鼠给药处理6h后行葡萄糖静脉试验(IVGTT)测定静脉全血葡萄糖和胰岛素水平,计算胰岛素、血糖曲线下面积(AUC).结果 正常组大鼠胰岛细胞有TIR4表达,LPS处理大鼠6h后胰岛组织中TLR4蛋白和mRNA的表达升高,与对照组比较差异具有统计学意义(P＜0.01),注射抗TLR4抗体预处理后用LPS刺激,LPS诱导的TLR4蛋白及mRNA水平的升高均被抑制(P＜0.01).LPS处理后与正常对照组比较,大鼠IVGTT 10、30、60、120 min血糖明显升高,30、60、120 min胰岛素分泌降低(P＜0.01).抗TLR4抗体干预后能降低30 ～ 120min血糖的升高,改善LPS对胰岛素分泌的抑制(P＜0.01).结论 脓毒症大鼠胰岛细胞TLR4表达升高,LPS-TLR4系统可能是应激性高血糖的发生机制.     

NF-κB p65 repression by the sesquiterpene lactone, Helenalin, contributes to the induction of autophagy cell death

Background

This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.

Methods

VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.

Results

Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.

Conclusions

These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.     

原发性浆细胞白血病(附11例报告)

目的　探讨原发性浆细胞白血病（ＰＰＣＬ）的临床特点。方法　对１１ 例ＰＰＣＬ进行回顾性分析。结果　７ 例接受治疗,除２ 例仍存活外（１例存活已达１ ａ 现仍在观察中,另１例为新近诊断）其余均已死亡（１例存活５ａ,其余均于１ ａ内死亡）。结论　ＰＰＣＬ与急性白血病类似。