全文获取类型
收费全文 | 898篇 |
免费 | 53篇 |
国内免费 | 41篇 |
专业分类
耳鼻咽喉 | 3篇 |
儿科学 | 35篇 |
妇产科学 | 19篇 |
基础医学 | 87篇 |
口腔科学 | 24篇 |
临床医学 | 98篇 |
内科学 | 213篇 |
皮肤病学 | 10篇 |
神经病学 | 58篇 |
特种医学 | 170篇 |
外科学 | 57篇 |
综合类 | 25篇 |
预防医学 | 30篇 |
眼科学 | 15篇 |
药学 | 79篇 |
中国医学 | 4篇 |
肿瘤学 | 65篇 |
出版年
2022年 | 3篇 |
2021年 | 14篇 |
2020年 | 6篇 |
2019年 | 6篇 |
2018年 | 21篇 |
2017年 | 17篇 |
2016年 | 14篇 |
2015年 | 14篇 |
2014年 | 24篇 |
2013年 | 22篇 |
2012年 | 31篇 |
2011年 | 41篇 |
2010年 | 32篇 |
2009年 | 42篇 |
2008年 | 25篇 |
2007年 | 44篇 |
2006年 | 25篇 |
2005年 | 21篇 |
2004年 | 22篇 |
2003年 | 16篇 |
2002年 | 15篇 |
2001年 | 15篇 |
2000年 | 15篇 |
1999年 | 15篇 |
1998年 | 51篇 |
1997年 | 47篇 |
1996年 | 39篇 |
1995年 | 32篇 |
1994年 | 25篇 |
1993年 | 34篇 |
1992年 | 11篇 |
1991年 | 13篇 |
1990年 | 10篇 |
1989年 | 28篇 |
1988年 | 20篇 |
1987年 | 22篇 |
1986年 | 13篇 |
1985年 | 22篇 |
1984年 | 14篇 |
1983年 | 15篇 |
1982年 | 15篇 |
1981年 | 16篇 |
1980年 | 18篇 |
1978年 | 9篇 |
1977年 | 8篇 |
1976年 | 7篇 |
1975年 | 5篇 |
1970年 | 3篇 |
1969年 | 2篇 |
1935年 | 2篇 |
排序方式: 共有992条查询结果,搜索用时 15 毫秒
21.
Gustavo Lucena Kortmann Verônica Contini Guilherme Pinto Bertuzzi Nina Roth Mota Diego Luiz Rovaris Vanessa Rodrigues Paixão-Côrtes Leandro Leal de Lima Eugenio Horacio Grevet Carlos Alberto Iglesias Salgado Eduardo Schneider Vitola Luis Augusto Rohde Paulo Belmonte-de-Abreu Claiton Henrique Dotto Bau 《European archives of psychiatry and clinical neuroscience》2013,263(3):181-188
Attention-deficit/hyperactivity disorder (ADHD) affects approximately 5 % of school-aged children and 2.5 % of adults. Genetic studies in ADHD have pointed to genes in different neurobiological systems, with relatively small individual effects. The mineralocorticoid receptor is the main receptor involved in the initial triggering of stress response. Therefore, its encoding gene (NR3C2) is a candidate for psychiatric disorder studies, including ADHD, and behavioral phenotypes. There is evidence that the Val allele of the MRI180V polymorphism (rs5522) increases the risk of depression, attention and cognitive deficits. We investigated the possible role of the mineralocorticoid receptor gene in the symptom dimensions and susceptibility to persistent ADHD. We compared genotype and allele frequencies in 478 adult patients with ADHD and 597 controls and symptom dimensions in 449 patients and 132 controls. Diagnoses were based on the DSM-IV criteria. ADHD symptom dimensions were investigated with SNAP-IV for ADHD severity and Barkley scales for severity and impairment. Carriers of the Val allele presented higher inattention, hyperactivity/impulsivity and impairment scores, while genotype and allele frequencies did not differ between patients and controls. These results are consistent with a possible link between genetic variations in the HPA axis and inattention and hyperactivity measures. 相似文献
22.
Microtubule reassembly in surface-activated platelets 总被引:2,自引:0,他引:2
It is generally accepted that a circumferential microtubule supports the discoid shape of resting platelets. The fate of the many-coiled polymer following platelet activation, however, has been a subject of considerable debate. Morphological investigations have suggested that the circumferential coils are constricted into tight rings around centrally concentrated organelles during platelet shape change. Biochemical studies employing colchicine-binding assays, on the other hand, have indicated that the bundle of microtubules dissolves almost completely within seconds after activation and reassembles in a new location one to four minutes later. The present study has accepted the latter hypothesis in order to examine the second part of the disassembly-reassembly theory proposed in biochemical studies. Platelets exposed to low temperatures sufficient to remove all microtubules were placed on glass slides and microscope grids to cause surface activation during rewarming. The combined stimuli of rewarming and surface activation might have been expected to cause more rapid assembly than warming alone or activation alone. This was not the case. Reassembly of microtubules during rewarming and simultaneous surface activation was not accelerated. In contrast to the constriction of microtubule rings observed during activation in control platelets, the diameters of coils that developed in chilled platelets one to two hours after rewarming and surface activation were twice those of control cells. 相似文献
23.
Cardoso AA; Schultze JL; Boussiotis VA; Freeman GJ; Seamon MJ; Laszlo S; Billet A; Sallan SE; Gribben JG; Nadler LM 《Blood》1996,88(1):41-48
Even if neoplastic cells express tumor associated antigens they still may fail to function as antigen presenting cells (APC) if they lack expression of one or more molecules critical for the induction of productive immunity. These cellular defects can be repaired by physiologic activation, transfection, or fusion of tumor cells with professional APC. Although such defects can be repaired, antitumor specific T cells may still fail to respond in vivo if they may have been tolerized. Here, human pre-B cell acute lymphoblastic leukemia (pre-B ALL) was used as a model to determine if primary human tumor cells can function as alloantigen presenting cells (alloAPC) or alternatively whether they induce anergy. In the present report, we show that pre-B cell ALL express alloantigen and adhesion molecules but uniformly lack B7-1 (CD80) and only a subset express B7-2 (CD86). Pre-B ALL cells are inefficient or ineffective alloAPC and those cases that lack expression of B7-1 and B7-2 also induce alloantigen specific T- cell unresponsiveness. Under these circumstances, T-cell unresponsiveness could be prevented by physiologic activation of tumor cells via CD40, cross-linking CD28, or signaling through the common gamma chain of the interleukin-2 receptor on T cells. Taken together, these results suggest that pre-B ALL may be incapable of inducing clinically significant T-cell-mediated antileukemia responses. This defect may be not only due to their inability to function as APC, but also due to their potential to induce tolerance. Attempts to induce clinically significant antitumor immune responses may then require not only mechanisms to repair the antigen presenting capacity of the tumor cells, but also reversal of tolerance. 相似文献
24.
25.
Suppression of the migration and invasion is mediated by triptolide in B16F10 mouse melanoma cells through the NF‐kappaB‐dependent pathway 下载免费PDF全文
Hui‐Yu Jao Fu‐Shun Yu Chun‐Shu Yu Shu‐Jen Chang Kuo‐Ching Liu Ching‐Lung Liao Bin‐Chuan Ji Da‐Tian Bau Jing‐Gung Chung 《Environmental toxicology》2016,31(12):1974-1984
Melanoma cancer is one of the major causes of death in humans worldwide. Triptolide is one of the active components of Tripterygium wilfordii Hook F, and has biological activities including induced cell cycle arrest and induction of apoptosis but its antimetastatic effects on murine melanoma cells have not yet been elucidated. Herein, we investigated the effect of triptolide on the inhibition of migration and invasion and possible associated signal pathways in B16F10 murine melanoma cancer cells. Wound healing assay and Matrigel Cell Migration Assay and Invasion System demonstrated that triptolide marked inhibiting the migration and invasion of B16F10 cells. Gelatin zymography assay demonstrated that triptolide significantly inhibited the activities of matrix metalloproteinases‐2 (MMP‐2). Western blotting showed that triptolide markedly reduced CXCR4, SOS1, GRB2, p‐ERK, FAK, p‐AKT, Rho A, p‐JNK, NF‐κB, MMP‐9, and MMP‐2 but increased PI3K and p‐p38 and COX2 after compared to the untreated (control) cells. Real time PCR indicated that triptolide inhibited the gene expression of MMP‐2, FAK, ROCK‐1, and NF‐κB but did not significantly affect TIMP‐1 and ‐2 gene expression in B16F10 cells in vitro. EMSA assay also showed that triptolide inhibited NF‐κB DNA binding in a dose‐dependent manner. Confocal laser microscopy examination also confirmed that triptolide inhibited the expression of NF‐κB in B16F10 cells. Taken together, we suggest that triptolide inhibited B16F10 cell migration and invasion via the inhibition of NF‐κB expression then led to suppress MMP‐2 and ‐9 expressions. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1974–1984, 2016. 相似文献
26.
Nina R. Mota Claiton H. D. Bau Tobias Banaschewski Jan K. Buitelaar Richard P. Ebstein Barbara Franke Michael Gill Jonna Kuntsi Iris Manor Ana Miranda Fernando Mulas Robert D. Oades Herbert Roeyers Aribert Rothenberger Joseph A. Sergeant Edmund J. Sonuga‐Barke Hans‐Christoph Steinhausen Stephen V. Faraone Philip Asherson 《American journal of medical genetics. Part B, Neuropsychiatric genetics》2013,162(6):546-549
27.
28.
Many people believe that euthanasia and assisted suicide are condoned carte blanche in the Netherlands. Not true. Both are formally forbidden by criminal law and can be administered only when certain procedures and criteria have been followed. Below, a look at the policies and practices regarding euthanasia and assisted suicide in The Netherlands and the role of nurses in this area. 相似文献
29.
Background
Genetic polymorphisms of the TCF7L2 gene are strongly associated with large increments in type 2 diabetes risk in different populations worldwide. In this study, we aimed to confirm the effect of the TCF7L2 polymorphism rs7903146 on diabetes risk in a Brazilian population and to assess the use of this genetic marker in improving diabetes risk prediction in the general population. 相似文献30.
Maarten E Witte Lars B Richard J Rodenburg Jeroen A Belien Rene Musters Thierry Hazes Liesbeth T Wintjes Jan A Smeitink Jeroen JG Geurts Helga E De Vries Paul van der Valk Jack van Horssen 《The Journal of pathology》2009,219(2):193-204
Mitochondrial dysfunction has been implicated in the development and progression of multiple sclerosis (MS) lesions. Mitochondrial alterations might occur as a response to demyelination and inflammation, since demyelination leads to an increased energy demand in axons and could thereby affect the number, distribution and activity of mitochondria. We have studied the expression of mitochondrial proteins and mitochondrial enzyme activity in active demyelinating and chronic inactive MS lesions. Mitochondrial protein expression and enzyme activity in active and chronic inactive MS lesions was investigated using (immuno)histochemical and biochemical techniques. The number of mitochondria and their co‐localization with axons and astrocytes within MS lesions and adjacent normal‐appearing white matter (NAWM) was quantitatively assessed. In both active and inactive lesions we observed an increase in mitochondrial protein expression as well as a significant increase in the number of mitochondria. Mitochondrial density in axons and astrocytes was significantly enhanced in active lesions compared to adjacent NAWM, whereas a trend was observed in inactive lesions. Complex IV activity was strikingly up‐regulated in MS lesions compared to control white matter and, to a lesser extent, NAWM. Finally, we demonstrated increased immunoreactivity of the mitochondrial stress protein mtHSP70 in MS lesions, particularly in astrocytes and axons. Our data indicate the occurrence of severe mitochondrial alterations in MS lesions, which coincides with enhanced mitochondrial oxidative stress. Together, these findings support a mechanism whereby enhanced density of mitochondria in MS lesions might contribute to the formation of free radicals and subsequent tissue damage. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. 相似文献