石杉碱甲类似物的研究II.N-甲基吡啶酮石杉碱甲类似物的合成

石杉碱甲(1)是从中草药石杉属植物千层塔(Lycopodium serratum Thunb.)中分得的一种高效可逆的乙酰胆碱酯酶抑制剂,临床试验证实它对早老性痴呆症有显著疗效。本文报道N-甲基吡啶酮石杉碱甲类似物2和3的合成。2-甲氧基-5-甲氧羰基-11-亚甲基-5,9-甲撑环辛-7-烯并吡啶(9)在乙腈中用三甲基氯硅烷和碘化钠选择性脱保护以定量的产率得吡啶酮10,再用甲醇钠和碘甲烷甲基化得N-甲基吡啶酮11,11经碱性水解,Curtius重排和氨基的脱保护得N-甲基吡啶酮石杉碱甲类似物2。通过类似的途径从中间体2-甲氧基-5-甲氧羰基-7-甲基-11-酮-5,9-甲撑环辛-7-烯并吡啶(14)合成了类似物3。类似物2和3的乙酰胆碱酯酶抑制活性均低于天然石杉碱甲。     

术中超声引导放射性^125Ⅰ粒子组织间植入治疗局部晚期胰腺癌

目的 探讨超声引导下术中放射性^125Ⅰ粒子组织间种植治疗无法切除胰腺癌的可行性和疗效。方法 2002年4月～2005年2月，我院行开腹^125Ⅰ粒子植入治疗晚期胰腺癌21例。术前根据治疗计划，确定粒子活度和种植粒子个数，肿瘤匹配周边剂量为65～110Gy，每颗粒子活度为0．4～0．5mCi。超声引导下插入粒子种植针，Mick粒子植入器植入^125Ⅰ粒子，粒子植入数10～75颗。8例粒子植入前或后行胃肠或胆肠吻合术。2例术后行外放疗联合单药吉西他滨化疗。1例术前置入支架，1例术后置入支架。结果 15例腹痛中，14例术后1～3d疼痛即开始缓解，其中7例疼痛完全缓解，7例部分缓解，1例无效，有效率93．3％（14／15）；除2例失访外，CR5例，PR7例，PD5例，NC2例。19例中位生存期5个月，1年生存率26．3％。1例出现乳糜漏，3例粒子移位到肝脏。无胰漏和胰腺炎等并发症。结论 放射性^125Ⅰ粒子组织间种植治疗胰腺癌具有安全、有效、创伤小和并发症发生率低等优点，是一种较好的补救治疗手段。     

蝎毒多肽对大鼠纤溶系统的作用

从蝎毒中获取具有多种药理活性的肽类混合物（简称ＳＶＰ），用下肢血管灌流和整体给药的动物模型，观察对血管灌流液内纤溶酶原激活物（ＰＡ）活性、血浆优球蛋白纤溶活性（ＥＦＡ）、纤溶酶（ＰＬ）活性的影响。结果：１～２０μｇ／ｍｌ灌流３ｍｉｎ和０．８，１．６ｍｇ·ｋｇ－１ｉｐＳＶＰ１ｈ血浆中ＰＡ活性与对照组相比明显升高（Ｐ＜０．０５或Ｐ＜０．０１），这可能与血管内皮细胞释放ＰＡ活性增加有关。     

The prevalence of LRRK2 Gly2385Arg variant in Chinese Han population with Parkinson's disease.

We conducted a case-control study to determine the prevalence of the LRRK2 Gly2385Arg variant in patients with Parkinson's disease in Han population in mainland China. Heterozygous LRRK2 Gly2385Arg variant was identified in 14 of 235 patients with Parkinson's disease (5.69%), but not in 214 unrelated healthy controls. Multivariate analysis indicated the frequency of Gly2385Arg variant in the female patients with early age at onset is higher than their male counterparts. The founder haplotype analysis showed the variant carriers shared the same founder. Clinically, the LRRK2 Gly2385Arg carriers presented with classical Parkinson's disease symptoms. Our study indicates that the LRRK2 Gly2385Arg variant is a potential ethnic-specific genetic risk factor of Parkinson's disease within Chinese Han ethnicity.     

北京石景山地区孕妇女婴幼儿血铅动态研究

在严格的质量保证措施下，北京石景山地区２７０名孕妇及其婴儿进行了血铅的追瞎调查。结果显示：孕３月血铅水平为４５．０μｇ／Ｌ，分娩前血铅水平为６４．８μｇ／Ｌ，孕期血铅呈升高趋势，其中后３月升高明显，分娩前血铅约为孕３月血铅的１．５倍；脐带血铅水平为５１．９μｇ／Ｌ，其中有１０．４％的胎儿脐血铅高于１００μｇ／Ｌ；     

T7 RNA聚合酶催化的荧光扩增技术检测人CEA

目的 建立检测人CEA的T7 RNA聚合酶催化的荧光扩增技术.方法 以亲和素作为连接分子,连接生物素化的检测抗体和生物素化的DNA,加入T7RNA聚合酶进行转录扩增反应,对生成的RNA产物进行荧光检测,并同时进行夹心ELISA方法检测人CEA.结果 成功的建立了检测人CEA的T7 RNA聚合酶催化的荧光扩增技术,其检测CEA的灵敏度达2×10 -3 ng/ml,比夹心EL ISA 方法灵敏度高125倍.结论 T7 RNA聚合酶催化的荧光扩增技术较夹心ELISA方法具有更高的敏感性,有可能作为一种新的检测方法用于临床的早期诊断.     

抗肿瘤药复方三生注射液的研究

复方三生注射液是由生附片等六味中药组成的静注用灭菌水溶液。本文综合报道了该制剂的制备方法,质量分析,抗肺癌作用的药理及临床等研究的主要内容。结果表明,本品是治疗中晚期肺癌有效的中药复方注射剂。     

Study on "massage to activate the meridian" apparatus in the treatment of cholecystolithiasis

This apparatus is designed in accordance with the doctrine and principles of traditional Chinese medicine and based on the theory of meridian and specific massage procedure in conjunction with the "Information Theory" and electron utilizing technique. It utilizes a programmed induction "Special Wave" to stimulate a series of acupuncture points, combined with administration of western drugs from a regime of "General Attack" treatment on the gallbladder stones, to enhance their evacuation. By stimulating the specific acupuncture points with this apparatus through the action of "Special Wave" strong contraction of the gallbladder can be elicitated. Experiments on dogs showed that under such stimulation the intra-gallbladder pressure can be increased 26 times, producing a jet of bile flow which facilitates the expulsion and evacuation of stones in the gallbladder. Method of use: first place a conductive rubber pad on the acupuncture point to be stimulated, then use a hand-held electrode to massage-stimulate the meridian. It is carried out forward and backward along the direction of meridian. The whole procedure is carried out under direct visualization with the help of ultrasonic B-scan. When dilatation of the common bile duct is observed, the hand-help electrode is used to perform push-compress massage on the meridian, and acupuncture points making the stones enter the common bile duct B-scan give proper orientation for the manipulation. This method has been tested clinically on 410 patients with good results. The evacuation rate of the gallbladder stones was 92.7% with complete evacuation achieved in 17.3%.(ABSTRACT TRUNCATED AT 250 WORDS)     

左心室局部心肌梗塞时的组分式模型及计算机仿真

为研究左心室局部心肌梗塞时心肌各部分的能量供需状况及心脏辅助装置对改善心肌能量供给的影响，我们建立了一个由左心室正常区域心肌和梗塞区域心肌两部分组成的组分式模型，我们的模型以Ｓｕｎａｇａｗａ的模型为基础，并在此基础上加以扩展，与Ｓｕｎａｇａｗａ的模型相比，我们的模型允许梗塞区域心肌的收缩性在一定范围内变化，从而可以模拟各种不同范围和不同程度的梗塞情况。用建立好的左心室组分式模型取代我们原有的狗的心