牛乳铁蛋白质量标准的研究

目的研究牛乳铁蛋白的质量标准。方法采用化学及高效液相色谱法(HPLC)进行定性鉴别； 采用HPLC法对牛乳铁蛋白的含量进行测定。结果牛乳铁蛋白的浓度在25 ～ 1 000 μg·mL^-1与其峰面积成良好的线性关系 ，r＝0.999 9。结论该法操作简便，精密度好，结果准确可靠，可作为牛乳铁蛋白质量标准的重要指标。     

尖吻蝮蛇毒中一种新类凝血酶的分离纯化

用阴离子交换色谱和凝胶过滤色谱技术,从尖吻蝮蛇毒中纯化得到一个具有凝血活性的组分.经Superdex 75 HR10/30预装柱检测,纯度达99.91%.SDS-PAGE显示为单一条带,还原、非还原条件下分子量分别为59.25k、52.58k.该组分的凝血酶比活为41.5u/mg,精氨酸酯酶比活为14.29u/mg,但不激活血浆凝血因子ⅩⅢ.EDTA不能抑制其凝血活性,而苯甲磺酰氟则产生不可逆抑制作用.结果表明该酶是一种新尖吻蝮蛇毒类凝血酶.     

通过改造工程细胞提高重组蛋白类药物产量的研究进展

哺乳动物细胞是表达蛋白类生物产品的理想宿主细胞。但是,表达水平常较低。通过对工程细胞进行改造,如抑制细胞凋亡、对细胞周期进行调控、提高翻译后加工能力、改变细胞新陈代谢的特性、实现基因组热点整合等,可有效地提高重组蛋白类药物的产量,为生物药品的规模化生产奠定基础     

内侧楔骨远端倾斜与拇外翻关系的相关性

目的通过直接测量所选定的足负重正位X线片上内侧楔骨远端倾斜的相关角度,研究其与拇外翻之间的相关关系。方法回顾性选取了中国人民解放军联勤保障部队第九二〇医院2018年1月至2021年2月期间影像科拍摄的足负重正位片,测量所有样本HVA并根据其大小将所有样本分为正常组、轻中度拇外翻组和重度拇外翻组,再测量每个样本的IMA、MAA、MCA、DMCA等,最后对2组的测量指标进行统计分析。结果共收集679例足负重正位片,左足330例(49%)、右足349例(51%),男足262例(39%),女足417例(61%);总患者数为538例,其中男性222例(41%),女性316例(59%);患者平均年龄38(18～82)岁,其中男性平均年龄28(18～73)岁、女性平均年龄42(18～82)岁。轻中度拇外翻组和重度拇外翻组中IMA、MAA、MCA均大于正常组,差异有统计学意义(P <0.05),且重度拇外翻组IMA和MCA均大于轻中度拇外翻组,差异有统计学意义(P <0.05);而DMCA在轻中度拇外翻组和重度拇外翻组中均与正常组之间无统计学差异(P>0.05)。结论内侧楔骨远端关节...     

乳铁蛋白对大鼠成骨细胞增殖与分化的影响

目的观察乳铁蛋白(Lf)对大鼠成骨细胞增殖分化的影响,初步探讨Lf对成骨细胞的作用机制。方法用不同浓度的Lf干预成骨细胞,在不同时间分别测定细胞数目的增殖、细胞内碱性磷酸酶活性,并用半定量RT-PCR法检测成骨细胞内RANKL/OPG mRNA基因的表达。结果Lf呈时间和浓度依赖性地促进大鼠成骨细胞增殖及碱性磷酸酶活性,每组实验在不同时间的差异均有显著的统计学意义(P<0.05),其中400μg/ml组在72h时细胞增殖数目达到最大。在碱性磷酸酶活性方面,400μg/ml组在72h时则有所下降。不同浓度Lf、不同作用时间均能促使成骨细胞中OPG mRNA表达增加,同时抑制RANKL mRNA表达。结论Lf能促进成骨细胞的增殖分化,并且能够调节成骨细胞RANKL/ OPG mRNA表达,从而抑制破骨细胞介导的骨吸收。     

At the heart of the problem: congestive cardiac failure as a cause of ascites: A narrative review

Heart failure leading to cardiac ascites is an extremely rare and underrecognized entity in clinical practice. Recognizing cardiac ascites can be difficult, especially since patients presenting with ascites may have more than 1 etiology. Various biomarkers are available to aid in the diagnosis of cardiac ascites, though with differing sensitivities and specificities. Such biomarkers include serum albumin, ascitic albumin and protein, as well as serum N-terminal pro-brain natriuretic peptide (NT-proBNP). While serum NT-proBNP is a powerful biomarker in distinguishing the etiology of ascites and monitoring treatment progression, its cost can be prohibitive in low-resource settings. Clinicians practicing under these circumstances may opt to rely on other parameters to manage their patients. We go on further to report a series of 3 patients with cardiac ascites to illustrate how these biomarkers may be employed in the management of this patient population. Clinicians should always keep in mind the differential diagnosis of cardiac failure as a cause of ascites. The resolution of cardiac ascites may serve as a surrogate clinical marker for response to antifailure therapy in lieu of NT-proBNP at resource-scarce centers.     

Two important factors affecting the prognosis of patients treated with sorafenib followed by regorafenib for advanced hepatocellular carcinoma

ObjectiveTo investigate the effects of hand–foot syndrome (HFS) and fatigue on disease progression and survival in patients treated with sorafenib followed by regorafenib for advanced hepatocellular carcinoma.MethodsA retrospective analysis of patients with advanced hepatocellular carcinoma treated with sorafenib in our hospital from 1 October 2018 to 31 October 2021 was performed, and clinical and pathological data and follow-up results were obtained. Patients were divided into groups according to the severity of HFS and fatigue. Survival analysis among the groups was performed using the Kaplan–Meier method, continuous variables were analyzed using the t-test, and factors associated with survival were evaluated using multivariate Cox regression analysis.ResultsThe study included 150 men and 23 women with a mean age of 60.77 years (range: 40–85 years). The median overall survival (OS), progression-free survival (PFS), and time to tumor progression (TTP) increased with increasing severity of HFS. Conversely, the median OS, PFS, and TTP decreased with increasing severity of fatigueConclusionHFS and fatigue were independent risk factors affecting TTP, PFS, and OS among patients treated with sorafenib followed by regorafenib for advanced hepatocellular carcinoma.     

距骨颈骨折的手术治疗

目的探讨距骨颈骨折的手术治疗及并发症防治。方法回顾2000年至2004年手术治疗的距骨颈骨折12例,总结其手术入路、内固定方法及术后处理。结果所有患者均在术后12~14d伤口拆线出院,无伤口感染、不愈合或皮肤坏死发生。12例患者中有10例获得随访,随访7个月至5年,平均3年4个月。按Hawkins标准优2例、良7例、中1例,创伤性骨关节炎4例,距骨无菌性坏死2例。结论对于复位不理想的距骨颈骨折应积极行切开复位内固定,强调骨折的解剖复位,避免术后过早负重,降低术后并发症的发生几率。     

中美婴儿运动表现测试常模差异分析

目的比较中美婴儿运动表现测试(TIMP)常模,明确本地化研究和修正的重要性,为指导TIMP在国内的推广提供依据。方法经TIMP手册正反译,评估人员培训、信度考核后,分别在华东、华北、华南、华中、西南、西北、东北区域11家医疗机构采样,建立中国TIMP常模,并就样本情况、得分情况及亚组分类与美国常模进行对比。结果中国TIMP常模共13个周龄组,样本总数为1 035例,其中男性占56%,女性占44%,汉族占比95%,少数民族5%,早产儿和足月儿分别占比31%、69%;美国常模共12个周龄组,样本总数为990例,其中男性占52%,女性48%,白种人占58%,黑种人25%,高危、中危、低危婴儿分别占比35%、30%、35%。中国TIMP常模各周龄组得分均值低于同周龄组的美国常模(P<0.01);中国常模第10、50、90百分位数曲线低于美国常模。中国常模足月儿与早产儿亚组对比,大多数周龄组得分无显著差异;美国常模中高危儿亚组得分显著低于低危组(P<0.001)。结论基于两国人口特征和儿童保健发展现状的不同,中美TIMP常模取样人群存在差异。中国常模各周龄组的得分均值低于美国常模同周...     

洪善贻治疗晚期肺癌经验

近年来我国肺癌发病率不断提高,虽然早期肺癌的检出率逐年提高,但晚期肺癌仍占多数.大量研究证明,合理的中医药干预能延长晚期肺癌患者生存期,提高生活质量.洪善贻主任中医师系第三批全国名老中医药专家学术经验继承工作指导老师、浙江省名中医,在宁波、舟山悬壶50余载,擅长恶性肿瘤及杂病的诊治.笔者有幸跟师学习数载,时受点拨,兹对...