迟发性佝偻病与支气管哮喘

目的 观察迟发性佝偻病并发哮喘的治疗反应,探讨维生Ｄ（ＶＤ）缺乏的哮喘发病中的作用。方法 ３１例迟发性佝偻病并发哮喘患儿随机分成Ａ、Ｂ两组,１５例单纯哮喘患儿为Ｃ组。３组均吸入二丙酸倍氯米松气雾剂（ｂｅｃｏｔｉｄｅ ｉｎｈａｌｅｒ）１年,Ａ组另加ＶＤ３治疗。于治疗前后别测定肺功能等。结果 治疗前３组用力呼气一秒量（ＦＥＶ１．０）及用力呼气峰流速（ＰＥＦ）均减低,但组间无差别;Ａ、Ｂ两组骨钙素和反映     

SETDB1: A perspective into immune cell function and cancer immunotherapy

Oncogene SET Domain Bifurcated 1 (SETDB1)/ESET, an H3K9 methyltransferase, was originally discovered over two decades ago; however, its function in the immune response was not first reported until 2011. SETDB1 immune functions include B cell maturation, T cell activity regulation, and immune escape in cancer cells. In B lymphocytes, SETDB1 mediates the transition from pro-B to pre-B cells and represses endogenous retroviruses (ERV) to encourage B cell lineage differentiation and maturation. SETDB1 alters T cell function by methylating IL-2 and IL-17 promoters and mediating T cell lineage commitment and development. In addition, SETDB1 plays a critical role in ERV silencing within a variety of immune cells, which can indirectly weaken the immune response. Although SETDB1 is critical for normal immune cell function, overexpression in cancer cells negatively impacts immune cell fights against cancer through decreased tumour immunogenicity. Within cancer cells, SETDB1 overexpression represses production and infiltration of antitumour immune cells, mediates immune escape through TE and ERV silencing, represses the type I interferon pathway, and interferes in immune checkpoint blockade (ICB) outcomes by regulation of PD-L1 expression and IFN signalling. In this review, we further discuss the immunological mechanisms of SETDB1 in normal and cancerous cells and its implications in cancer immunotherapy.     

Characterization and phylogenetic analysis of a neurovirulent Zika virus isolated from Cambodia in 2019

The geographic range of Zika virus (ZIKV) has expanded from Asia to the Americas, leading to the 2015–2016 pandemic with enhanced neurovirulence. At present, ZIKV is continuously circulating in many Southeast Asian countries. Unfortunately, the persistent evolution of ZIKV in Southeast Asia and its influence on the biological characteristics of the virus remain incompletely understood. In this study, the in vitro and in vivo properties of a new ZIKV isolate obtained from Cambodia in 2019 (CAM/2019) were characterized and compared with those of the Cambodian strain (CAM/2010). Compared with CAM/2010, the CAM/2019 virus showed similar plaque morphology and growth curves in cell cultures and induced comparable viremia and organ viral loads profiles in both BALB/c and A129 (IFNAR1^−/−) mice upon intraperitoneal (i.p.) inoculation. Remarkably, the CAM/2019 virus exhibited enhanced neurovirulence in neonatal mice compared with CAM/2010, with a 74-fold reduction in the 50% lethal dose (LD₅₀). Consistently, CAM/2019 produced higher viral loads in the brains of BALB/c neonatal mice than CAM/2010 did. Sequence alignment showed that the CAM/2019 virus has acquired 12 amino acid substitutions, several of which were found to be associated with neurovirulence. In particular, the CAM/2019 virus shared an A1204T substitution in NS2A with the Thai isolate SI-BKK02 that was isolated from a microcephaly case. Taken together, our results indicate that a ZIKV strain isolated with specific mutations has emerged in Cambodia, highlighting the need for extensive molecular and disease surveillance in Cambodia and other Asian countries.     

Screening and identification of 3-aryl-quinolin-2-one derivatives as antiviral agents against influenza A

Quinolin-2-one represents an important and valuable chemical motif that possesses a wide variety of biological activities; however, the anti-influenza activities of quinolin-2-one-containing compounds were rarely reported. Herein, we describe the screening and identification of 3-aryl-quinolin-2-one derivatives as a novel class of antiviral agents. The 3-aryl-quinolinone derivatives were synthesized via an efficient copper-catalyzed reaction cascade that we previously developed. Using this synthetic method, preliminary structure–activity relationships of this scaffold against the influenza A virus infection were systematically explored. The most potent compound 34 displayed IC₅₀ values of 2.14 and 4.88 μM against the replication of H3N2 (A/HK/8/68) and H1N1 (A/WSN/33) strains, respectively, without apparent cytotoxicity on MDCK cells. We further demonstrated that 27 and 34 potently inhibited the plaque formation of the IAV, rendering this scaffold attractive for pursuing novel anti-influenza agents.     

痢疾杆菌R质粒的检测、消除及药物敏感试验

目的：探讨得疾杆菌的耐药性。方法：采用Ｋ－Ｂ法测定痢疾杆菌的耐药谱,对耐药菌株进行Ｒ闰的检测及接合传递人工诱导痢疾杆功秋稳定Ｌ型,观察Ｒ质粒丢失情况。结果：４５株痢疾杆菌４４经,耐药率９７．８％,其中三重耐药菌株占９３．２％,四重以上耐药菌株占７３．３％。８８．９％（４０／４５）的菌株检测出Ｒ质粒,质粒的接合传递率为６１．４％。痢疾杆菌Ｌ型未见质粒丢失。结论：痢疾杆菌的耐药性十分严重,自由质粒控制     

Effect of intranasal structure-normalizing surgery on allergic rhinitis: A primary report of 41 cases

The heatment of allergic rhinitis is yet qUite a challeopng and intrigUing Pchem, and there has not beeneradicable modality. Dings often relieve symptomS tempowhly. Selechve nerve block surgerys such as section andelectroCauterization of Vidian nerve, anterior ethlnoidalnerve and sphenoplatine nerve, have brought us a newProspect, but long-term results still need checking andthey are not considend to be adopted until Ph~otherapy and ilnlnunotherapy come to failu.[l]. Clinically, allergic rh…     

PTF—V1在二尖瓣狭窄球囊扩张术中的临床效果分析

目的 探讨二尖瓣狭窄患者行经皮二尖瓣球囊扩张术（ＰＢＭＶ０前后心电图ＰＴＦ－Ｖ１的变化及其与血流动力学各指标的相关性。方法 分析２９例窦性心律的二尖瓣狭窄患者ＰＢＭＶ前后ＰＴＦ－Ｖ１的变化,并与二尖瓣面积（ＭＶＡ）、左房平均压（ＭＬＡＰ）和左房内径（ＬＡＤ）作相关分析。结果 ＰＢＭＶ术后ＰＴＦ－Ｖ１显著改善（Ｐ〈０．０１）,与ＭＬＡＰ、ＬＡＤ呈显著正相关,与ＭＶＡ呈显著负相关（Ｐ〈０．０５）。结论     

成年大鼠纹状体、边缘区和苍白球的计算机三维重建

目的　建立和了解成年大鼠纹状体、边缘区和苍白球在脑中的立体形态和相互关系 .方法　在大鼠脑的连续冠状切片 Nissl染色的基础上通过 Onyx2超级图形工作站应用计算机图形技术进行了三维重建 .结果　边缘区位于尾壳核和苍白球之间 ;尾壳核的立体形态呈近似的内凹半球形 ,从嘴侧到尾侧随着脑平面的增宽尾壳核逐渐向外侧 (即靠近外轮廓的方向 )移位 ,体积为 (37.77± 9) m m3,最大嘴尾径为 6 .2mm;最大背腹径为 4.9m m;最大内外径为 3.5 2 9mm.苍白球呈块形 ,位于尾壳核的内侧 ,除内侧外其他三个方向均被尾壳核包绕 ,体积为 (4.0 5± 0 .0 6 ) mm3,最大嘴尾径为 4.41mm,最大背腹径为 2 .6 45 mm ,最大内外径为 1.5 44 m m.边缘区呈现一个片状扇形结构 ,体积为 (0 .48± 0 .0 2 ) mm3,最大嘴尾径为 1.6 m m,最大背腹径为 2 .15 8m m,最大内外径为0 .17mm;同尾壳核和苍白球一样从嘴侧到尾侧随着脑平面的增宽边缘区亦逐渐向外侧 (即靠近外轮廓的方向 )移位 ,其移位的幅度亦明显大于脑平面增宽的幅度 ;整个边缘区从嘴侧到尾侧呈均匀变化 ,其片状逐渐变宽 ,长度 (背腹径 )逐渐变小 ,从而形成一个盘状结构 .结论　建立了大鼠脑纹状体、边缘区和苍白球的三维立体结构     

脐血临床应用前的实验研究

对５８例脐血红细胞进行了计数和血红蛋白含量测定,并测定乙肝两对半、丙肝抗体、人类免疫缺陷病毒抗体、肝功能及Ｔ细胞亚群分类和细菌培养。结果表明无传染病的正常孕妇分娩时的脐血,可作血源供临床使用并能用于治疗多种原因引起的贫血。