Endothelial progenitor cells modulated by IL-1β in multiple organ dysfunction syndrome in porcine

Objective To investigate the modulation of EPCs by interleukin 1β (IL-1β) and p38 mitogen activated protein kinase (p38MAPK) and the pathogenesis resulting from their dysdifferenfiation after trauma.Method Thirty pigs were divided into a control group (n = 15) and a multiple organ dysfimction syndrome (MODS) group (n = 15), the latter of which were subjected to a "two-hit" injury including hemon'hagic shock and endotoxemia. Phosphorylation of p38MAPK in peripheral blood mononuclear cells was monitored by western blotting. The concentration of IL-1β in peripheral blood plasma was determined by ELISA and the numbers of EPCs with FCM in peripheral blood plasma were monitored. The morbidity rates in the two groups were compared by chi square test. The levels of phosphorylation of p38MAPK in peripheral blood mononuclear cells, the concentmtions of IL-1β in peripheral blood plasma and the numbers of EPCs in the peripheral blood were compared between groups using with Student's t lest. Results The level of p38MAPK phosphorylation was more augmented and the concen-tration of IL-1β higher in peripheral blood mononuelear cells and plasma from MODS pigs compared with those from control pigs; nevertheless the mauler of EPC conspicuously decreased in the peripheral blood (P <0.01). The morbidity rate in the MODS group was much higher than that in the control group (P < 0.01). There were fewer EPCs in the peripheral blood of animals in group M than in the peripheral blood of animals in group C (P <0.01). Conclusions p38MAPK phosphorylation is important for the pathogenesis of MODS. p38MAPK phospho-rylation might cause the concentration of IL-1β in the peripheral blood plasma to rise and could cause a drop in the numbers of EPCs, thereby aggravating the inflanmmatory reaction in MODS.     

小型钛板坚强内固定治疗下颌骨骨折的临床经验

目的探讨小型钛板坚强内固定治疗下颌骨骨折的治疗效果。方法回顾分析273例下颌骨骨折患者应用小型钛板坚强内固定的治疗效果及随访结果,总结有关处理经验和教训。结果 273例中269例患者术后伤口Ⅰ期愈合,1例伤口感染、3例伤口延期愈合。主要并发症包括术后咬合关系紊乱10例,骨折愈合不良致骨折再错位1例,重度开口困难1例,手术造成牙根损伤3例、颏神经损伤1例。术后3个月作疗效评价。Ⅰ级:伤口愈合好,骨折端对位好无错位愈合,咬合关系正常,无开口困难等功能障碍;Ⅱ级:伤口愈合良好,骨折端无错位愈合或轻微错位,咬合关系基本正常,无或轻度开口功能障碍;Ⅲ级:伤口感染骨折断端不愈合,骨折端复位差错位愈合,咬合关系紊乱,有重度开口困难等功能障碍;3个等级分别为Ⅰ级占91.21%(249/273),Ⅱ级占6.96%(19/273),Ⅲ级占1.83%(5/273)。结论下颌骨骨折应用小型钛板坚强内固定治疗效果可靠,通过完善的围手术期处理,可以达到较好的临床疗效。     

升清降浊胶囊对慢性肾衰模型大鼠的保护作用及血清SOD、MDA的影响

目的探讨升清降浊胶囊对慢性肾衰模型大鼠的保护作用及抗氧化作用。方法在成功复制大鼠慢性肾功能衰竭模型的基础上,给予不同剂量升清降浊胶囊治疗,测定大鼠红细胞、血红蛋白、血肌酐及尿素氮(BUN)以及血清超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果升清降浊胶囊能够升高慢性肾衰模型大鼠全血中红细胞、血红蛋白水平,降低血肌酐及尿素氮水平,能够升高大鼠血清SOD含量,降低MDA的含量。结论升清降浊胶囊能改善肾衰模型大鼠肾功能指标,具有保护肾脏的作用,其机制可能与其提高大鼠抗氧化能力有关。     

槲皮素固体脂质纳米粒的制备与含量测定

目的建立槲皮素固体脂质纳米粒的制备工艺与含量测定方法。方法以山嵛酸甘油酯、胆固醇、大豆卵磷脂为载体材料,丙酮 三氯甲烷（1：1）为有机相,以泊洛沙姆、聚乙二醇、聚山梨酯80、纯化水为水相,乳化温度75 ℃,搅拌器转速600 r&#8226;min 1,冰水固化温度2 ℃进行制备;采用高速离心 高效液相色谱法测定槲皮素含量,色谱柱：Diamonsil C18柱（250 mm×4.6 mm,5 μm）;流动相：甲醇 4.3%乙酸溶液（55：45）,流速1.0 mL&#8226;min 1;检测波长254 nm,进样量20 μL,柱温30 ℃。结果制得固体脂质纳米粒稳定,槲皮素在2.0～200.0 μg&#8226;mL 1范围内线性关系良好,A=1.343 5C－1.490 4（r =0.999 6）,平均回收率为97.7%,RSD=1.09%（n =6）。结论所建制备工艺稳定,含量测定方法便捷,可靠,准确,可行。     

直肠癌全系膜切除及双吻合器术后吻合口瘘的相关因素分析与防治对策

目的探讨直肠癌行全系膜切除及双吻合器手术切除后吻合口瘘的相关原因及预防、诊断、治疗措施。方法回顾性分析我院2001年1月—2009年6月252例(男159例,女93例)直肠癌患者,行直肠全系膜切除及双吻合器术后吻合口瘘的发生率及治疗情况。结果 该组病例吻合口瘘的发生率为5.6%(14/252);确诊吻合口瘘发生时间为4~9 d,平均为6.8 d。其中8例高流量瘘采用早期横结肠造瘘与腹腔冲洗引流手术;6例低流量瘘采用非手术治疗,骶前双腔管冲洗与肛管固定引流术。2次治疗至出院时间为28~80 d,平均为48.6 d。术后吻合口瘘发生与患者的全身情况、吻合技术、吻合口位置,肿瘤Dukes分期、术前放化疗、是否行预防性造瘘等有相关性。结论术前充分的评估,术中细致的操作及选择性预防性造瘘的应用和围术期合理的处理是预防术后吻合口瘘的关键;术后及时正确的判断,恰当的手术时机的把握,充分的引流、营养支持等综合性措施是治疗吻合口瘘的必要手段。     

老年人结肠癌并急性肠梗阻86例外科处理分析

目的探讨老年人结肠癌并发急性肠梗阻的特点、诊断、外科处理方法与预后。方法回顾性分析我院2000年1月—2009年8月86例年龄>60岁的老年患者手术治疗的结肠癌并发急性肠梗阻病人的临床资料,其中一期切除吻合58例(右半结肠一期切除吻合39例,左半结肠一期切除吻合19例),分期手术19例(右半结肠4例,左半结肠15例),行Hartmann手术。肿瘤无法切除行单纯结肠造瘘和捷径手术9例。结果术后出现并发症8例,发生率为9.3%,其中切口感染3例,腹腔感染2例,肺部感染2例,感染性休克1例,1例死亡,病死率1.2%,85例痊愈出院。结论老年人结肠癌致肠梗阻的外科治疗要根据老年病人的具体情况选择合理的术式,准确的早期诊断,恰当的围术期处理,正确的手术方式的选择,是提高其预后的关键。     

双源CT成像评价冠状动脉病变的临床价值

目的 通过双源CT对冠脉检查与选择性冠状动脉造影的对比分析,探讨DSCT对冠脉病变诊断的意义.方法 55例同期行双源CT冠状动脉血管成像及选择性冠状动脉血管造影(CAG)检查的患者,分析比较DSCTCA的敏感性、特异性及准确度.结果 (1)DSCT冠状动脉造影按病例计算.诊断冠心病的敏感度、特异度、阳性和阴性预测值、准确度分别为97.7%、72.6%、93.5%、88.9%和92.7%;按病变血管计算,其敏感度、特异度、阳性和阴性预测值、准确度分别为94.9%、95.8%、92.5%、97.1%、95.5%;按可评价节段计算,检测节段病变总的敏感度、特异度、阳性和阴性预测值、准确度分别为88.2%、96.9%、90.5%、96.1%、94.7%.(2)DSCT造影与选择性冠脉造影比较对冠心病的诊断的差别无统计学意义;对诊断各支血管的敏感度、特异度、阳性和阴性预测值、准确度的差别无统计学意义.结论 双源CT对冠心病的诊断准确度接近选择性冠状动脉造影.在冠状动脉疾病的筛查方面,DSCTA在一定程度上可替代CAG.     

内皮祖细胞在创伤后多器官功能障碍中的变化及意义

Objective To investigate characteristics of changes in bone marrow endothelial progenitor cells (EPCs) and implications on multiple organ dysfunction syndrome (MODS) as a result of trauma.Methods Eighteen mini-pigs were randomized into two groups: MODS group (n=9) and control group (n=9).The animal models of MODS were reproduced by "two-hit" injury with hemorrhagic shock and lipopolysaccharide (LPS) injection.Bone marrow and peripheral blood of them were collected at five time points: normal condition (T1), before injection of LPS (T2), and 0 (T3), 24 (T4) and 48 hours (T5) after injection of LPS.Erythrocytic lysate was added to the samples, and the number of leucocytes in every sample was counted.The rate of EPCs in each sample was determined by flow cytometry.Number of EPCs in bone marrow and peripheral blood were calculated, and the results were analyzed statistically.Results The number of EPCs (×106/L) in bone marrow of control group at T1-5 was 7.64±0.68,7.32±0.55, 7.58±1.13, 7.77±0.70, and 7.88±0.84, respectively, and in peripheral blood control group was 3.54±0.26,4.06±0.64, 3.74±0.55, 3.61±0.37, and 3.98±0.63, respectively.The number of EPCs (×106/L) in bone marrow in the experimental group was 7.45 ± 1.55, 6.58 ± 0.80, 11.27 ± 1.20, 10.88±1.15, and 8.36 ± 2.88, respectively.The number of EPCa (× 106/L) in peripheral blood in the experimental group was 3.21±0.48, 8.71±2.04, 5.98±0.77, 1.27±0.91, and 2.14±0.96, respectively.The number of EPCs in bone marrow of experimental group was larger than that of control group at T3, T4, T5.The number of EPCs in the experimental group in peripheral blood was larger than that of control group at T2, T3, T4, T5.The number of EPCs in bone marrow was larger than that in peripheral blood at every time point (all P＜0.05).Conclusion The number of EPCs in peripheral blood elevates sharply in the earlier period, then plummetes quickly during MODS after a trauma.While the number of EPCs in bone marrow descends mildly at first, then rises obviously.Along with the aggravation of MODS, a declination of EPCs in bone marrow emerges.The change in bone marrow EPCs plays an important role in recovery of MODS.     

自酸蚀剂材料在口腔修复正畸托槽粘接中的应用

目的：总结近年有关自酸蚀剂粘接正畸托槽的现状。 方法：由作者应用计算机检索万方数据库中与使用自酸蚀剂粘接正畸托槽有关的文献,检索时限为2000-09/2010-08。检索关键词：自酸蚀剂;托槽;抗剪切强度。纳入标准：①选入针对性强,相关度高的文献。②排除一些重复性研究。对资料进行初审,并查看每篇文献后的引文。 结果：共20篇文献符合标准,自酸蚀剂粘接剂与传统釉质粘接剂比较,正畸托槽的抗剪切强度相近。两种方法在托槽脱落后的釉质粘接材料残留无显著差异。 结论：自酸蚀剂与传统釉质酸蚀粘接剂比较,其抗剪切强度可靠,粘接剂残留指数低,能满足粘接正畸托槽的需要。     

腹腔镜胆囊切除术中胆道损伤的预防和处理

目的探讨腹腔镜胆囊切除术（laparoscopic cholecystectomy,LC）致胆道损伤的原因、预防措施和处理方法。方法对7例LC引起的胆道损伤病例进行回顾性分析。结果7例胆道损伤病例中,术中钛夹误夹闭胆总管1例;3例Mirizzi综合征致胆总管损伤;右肝管损伤2例,其中1例是由于胆囊管变异所致;1例因胆囊颈部粘连严重,分离中在肝门处断离左肝管,并损伤右肝管。所有病例均行胆总管修补或端端吻合术,T管引流获得成功。结论LC引发的胆道损伤应引起足够的重视。发现不及时或处理不当都可能引起严重并发症。术者经验、局部粘连严重和胆道变异是胆道损伤的主要原因。可采用胆总管修补成形、端端吻合术或胆总管空肠Roux-en—Y吻合术治疗胆道损伤。