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91.
Seminal plasma alpha-glucosidase activity and male infertility 总被引:7,自引:1,他引:7
Mahmoud AM; Geslevich J; Kint J; Depuydt C; Huysse L; Zalata A; Comhaire FH 《Human reproduction (Oxford, England)》1998,13(3):591-595
Measurement of alpha-glucosidase (alpha-GLUC) activity by means of a simple
colorimetric test using a commercial kit (EpiScreen; FertiPro, Lotenhulle,
Belgium) yielded results that were strongly correlated with the values
obtained for the neutral iso-enzyme measured by a fluorimetric reference
method (r=0.85, P=0.003, n=13). The former method was characterized by a
low intra- and inter-coefficient of variation (6.6 and 4.3% respectively).
Vasectomized men with azoospermia (n=27) had a significantly lower
alpha-GLUC activity in semen than vasectomized men with residual
spermatozoa present (n=11, P < 0.01) and men with azoospermia of primary
testicular origin (n=33, P < 0.01). Receiver operating curve (ROC)
analysis showed alpha-GLUC measurement to be reasonably accurate in
differentiation between cases with obstructive versus testicular
azoospermia at criterion value 13.5 U/l (sensitivity=82%, specificity=
70%). In cases with spermatozoa present, alpha-GLUC activity and output per
ejaculate were positively correlated with sperm concentration (r=0.53 and
0.38, n=472), linear velocity (r=0.35 and 0.30, n=224), curvilinear
velocity (r=0.32 and r=0.29, n=224), semen adenosine triphosphate (r=0.35
and 0.26, n=64), the concentration of 5alpha-dihydrotestosterone (r=0.31
and 0.29, n=74), and gamma-glutamyltransferase activity (r=0.62 and 0.32,
n=275) in seminal plasma. The activity of alpha-GLUC was inversely
correlated with ROS generation after 12-myristate, 13-acetate phorbol ester
stimulation (r=-0.27, n=104), and both alpha-GLUC activity and total output
were inversely correlated with the concentration of peroxidase- positive
white blood cells among samples with > or =1x10(6)/ml of these cells
(r=-0.30 and -0.19, n=165). It is concluded that simple photometric
measurement of alpha-GLUC activity in seminal plasma reflects the
functional state of the epididymis and may be helpful for the differential
diagnosis of certain cases with azoospermia.
相似文献
92.
The gradual increase in follicle stimulating hormone (FSH) concentrations
in women approaching menopause results from the depletion of the ovarian
follicular pool, a process referred to as 'ovarian ageing'. This study
investigates whether variable endogenous FSH concentrations, as have been
observed in normo-ovulatory young women, are related to menstrual cycle
characteristics, including predictors of ovarian ageing. Serum
concentrations of immunoreactive FSH, oestradiol, and inhibin-A and
inhibin-B were measured, and follicular growth was assessed by transvaginal
ultrasound throughout the follicular phase in 39 healthy volunteers (20-35
years) with regular menstrual cycles. Median serum FSH concentration on
cycle day 3 was 5.1 IU/l (range 3.6-11.2), and median maximum follicular
phase FSH was 6.2 IU/l (range 4.3-11.2), observed on cycle day 6 (range
2-15). Maximum FSH concentrations were not correlated with age or cycle
length, nor with maximum inhibin-B. The number of small (<10 mm) antral
follicles on cycle day 3 was 11 (range 4-21) and was not correlated with
age, nor with maximum FSH. Inhibin-A remained low until a significant rise
on cycle day 9 (range 3-12), which was significantly correlated with the
late follicular rise in oestradiol (r = 0.56, P = 0.01). These observations
indicate a lack of correlation between maximum follicular phase serum FSH
concentrations and parameters of ovarian ageing in women under the age of
35 years. In addition, FSH concentrations assessed on cycle day 3 represent
an underestimation of maximum early follicular phase FSH. Distinct
individual differences in intra-ovarian modification of FSH action,
resulting in differences in the FSH threshold for stimulation of ovarian
function, may be operative.
相似文献
93.
T. Koller MAS msk ZFH OMT svomp® Dipl.-Physiotherapeut FH H. Luomajoki 《Der Orthop?de》2013,42(6):449-452
There is evidence that phantom pain is associated with a disrupted organization of the sensory cortex and that this organization can be normalized by training with two-point discrimination (TPD). In this case study a reduction in all three phantom modalities (i.e. phantom pain, phantom feeling and painful phantom sensation) and a reduction in pain level from m=?4.13/10 visual analogue scale (VAS) to m=?0.67/10 (VAS) could be observed in a patient with an upper limb amputation during a test period of 28 days with TPD. The quality of life and performance increased significantly. This can be a promising indication for a better social and work reintegration. 相似文献
94.
P Bavi Z Jehan R Bu S Prabhakaran N Al-Sanea F Al-Dayel M Al-Assiri T Al-Halouly R Sairafi S Uddin K S Al-Kuraya 《British journal of cancer》2013,109(10):2735-2743
Background:
Recently, the anaplastic lymphoma kinase (ALK) has been found to be altered in several solid and haematological tumours. ALK gene copy number changes and mutations in colorectal cancers (CRCs) are not well characterised. We aimed to study the prevalence of ALK copy number changes, translocations, gene mutations and protein expression in 770 CRC patients, and correlate these findings with molecular and clinico-pathological data.Methods:
ALK gene copy number variations and ALK expression were evaluated by fluorescence in situ hybridisation (FISH) and immunohistochemistry, respectively.Results:
Translocations of the ALK gene were not observed; 3.4% (26 out of 756) of the CRC patients tested had an increase in ALK gene copy number either amplification or gain. Interestingly, increased ALK gene copy number alteration was associated with poor prognosis (P=0.0135) and was an independent prognostic marker in multivariate Cox proportional hazards model. The study reveals a significant impact of ALK gene copy number alterations on the outcome of patients with CRC.Conclusion:
The findings of our study highlight a potential role of targeting ALK in advanced CRCs by using ALK FISH and ALK IHC as a screening tool to detect ALK alterations. Based on these findings, a potential role of ALK inhibitor as a therapeutic agent in a subset of CRC merits further investigation. 相似文献95.
Uddin S Ahmed M Hussain A Abubaker J Al-Sanea N AbdulJabbar A Ashari LH Alhomoud S Al-Dayel F Jehan Z Bavi P Siraj AK Al-Kuraya KS 《The American journal of pathology》2011,178(2):537-547
To identify genes potentially playing an important role in the progression of colorectal carcinoma (CRC), we screened global gene expression using cDNA expression array on 41 CRC tissue samples and 25 noncancerous colorectal tissue samples. Among the up-regulated genes, forkhead box M1 (FOXM1) has been shown to play a critical role in pathogenesis of various malignancies. Using immunohistochemistry on 448 Saudi CRC samples in tissue microarray format, FoxM1 protein overexpression was seen in 66% of CRC tissues and was significantly associated with poorly differentiated and highly proliferative tumors (P = 0.0200 and 0.0018, respectively). FoxM1 expression was also significantly associated with MMP-9 protein expression (P = 0.0002). In vitro data using CRC cell lines showed that inhibition of FoxM1 by thiostrepton resulted in inhibition of proliferation and induction of apoptosis in a dose-dependent manner. Overexpression of FoxM1 potentiated cell proliferation, cell transformation, and migration/invasion of CRC cells via up-regulation of FoxM1 target genes MMP2 and MMP9 and protected these cells from thiostrepton-mediated antiproliferative effects. Finally, in vivo, overexpression of FoxM1 promoted growth of CRC-cell line xenograft tumors in nude mice. Altogether, our data indicate that FoxM1 signaling contributes to aggressiveness in a subset of CRC and that the FOXM1 gene may serve as a useful molecular biomarker and potential therapeutic target. 相似文献
96.
DL Smit AR Mensenkamp S Badeloe MH Breuning MEH Simon KY Van Spaendonck CM Aalfs JG Post S Shanley IPC Krapels LH Hoefsloot RJA Van Moorselaar TM Starink J‐P Bayley J Frank MAM Van Steensel FH Menko 《Clinical genetics》2011,79(1):49-59
Smit DL, Mensenkamp AR, Badeloe S, Breuning MH, Simon MEH, van Spaendonck KY, Aalfs CM, Post JG, Shanley S, Krapels IPC, Hoefsloot LH, van Moorselaar RJA, Starink TM, Bayley J‐P, Frank J, van Steensel MAM, Menko FH. Hereditary leiomyomatosis and renal cell cancer in families referred for fumarate hydratase germline mutation analysis. Heterozygous fumarate hydratase (FH) germline mutations cause hereditary leiomyomatosis and renal cell cancer (HLRCC), an autosomal dominant syndrome characterized by multiple cutaneous piloleiomyomas, uterine leiomyomas and papillary type 2 renal cancer. The main objective of our study was to evaluate clinical and genetic data from families suspected of HLRCC on a nationwide level. All families referred for FH mutation analysis in the Netherlands were assessed. We performed FH sequence analysis and multiplex ligation‐dependent probe amplification. Families with similar FH mutations were examined for haplotype sharing. In 14 out of 33 families, we identified 11 different pathogenic FH germline mutations, including 4 novel mutations and 1 whole‐gene deletion. Clinical data were available for 35 FH mutation carriers. Cutaneous leiomyomas were present in all FH mutation carriers older than 40 years of age. Eleven out of 21 female FH mutation carriers underwent surgical treatment for symptomatic uterine leiomyomas at an average of 35 years. Two FH mutation carriers had papillary type 2 renal cancer and Wilms' tumour, respectively. We evaluated the relevance of our findings for clinical practice and have proposed clinical diagnostic criteria, indications for FH mutation analysis and recommendations for management. 相似文献
97.
98.
Alhussein J Alzahrani El-Harith A El-Harith Jutta Milzer Obeid E Obeid Manfred Stuhrmann Adel Al-Dayel Eltayeb A Mohamed Siddig Al-Egail Majid Daoud Ayub Chowdhury Adnane Guella Ibrahim Aloraifi Thomas F Schulz 《Nephrology, dialysis, transplantation》2005,20(11):2532-2536
BACKGROUND: Human herpes virus-8 (HHV-8) is a herpes virus that is always associated with Kaposi's sarcoma. Previous studies suggested a high rate of Kaposi's sarcoma in renal transplant patients in Saudi Arabia. The aim of this study was to investigate the prevalence of HHV-8 in Saudi renal transplant recipients and healthy controls. METHODS: An immunofluorescence technique was used to detect antibodies to the latent nuclear antigen (LANA) of HHV-8 in renal transplant patients, members of a family affected with Kaposi sarcoma, as well as healthy controls. RESULTS: A significantly higher HHV-8 seroprevalence was detected in renal transplant recipients from Saudi Arabia (27 out of 150; 18%) and in members of a family affected with Kaposi sarcoma (seven out of 10; 70%) relative to the seroprevalence in healthy controls (10 out of 577; 1.7%). Seropositivity for HHV-8 in these transplant patients was not significantly influenced by: the existence of relatives with kidney failure, the donors' country of origin, the recipients' home region within Saudi Arabia, the haemodialysis centre, the time that elapsed since the renal transplantation operation and the immunosuppressive regimen used. CONCLUSION: The present results provide some explanation for the previously noted high incidence of Kaposi's sarcoma in Saudi transplant patients. 相似文献
99.
100.
BL Foster HW Tso AB Tran FH Nociti Jr S Narisawa MC Yadav MD McKee JL Millán MJ Somerman 《Journal of bone and mineral research》2013,28(2):271-282
Tissue‐nonspecific alkaline phosphatase (TNAP) is expressed in mineralizing tissues and functions to reduce pyrophosphate (PPi), a potent inhibitor of mineralization. Loss of TNAP function causes hypophosphatasia (HPP), a heritable disorder marked by increased PPi, resulting in rickets and osteomalacia. Tooth root cementum defects are well described in both HPP patients and in Alpl?/? mice, a model for infantile HPP. In Alpl?/? mice, dentin mineralization is specifically delayed in the root; however, reports from human HPP patients are variable and inconsistent regarding dentin defects. In the current study, we aimed to define the molecular basis for changes in dentinogenesis observed in Alpl?/? mice. TNAP was found to be highly expressed by mature odontoblasts, and Alpl?/? molar and incisor roots featured defective dentin mineralization, ranging from a mild delay to severely disturbed root dentinogenesis. Lack of mantle dentin mineralization was associated with disordered and dysmorphic odontoblasts having disrupted expression of marker genes osteocalcin and dentin sialophosphoprotein. The formation of, initiation of mineralization within, and rupture of matrix vesicles in Alpl?/? dentin matrix was not affected. Osteopontin (OPN), an inhibitor of mineralization that contributes to the skeletal pathology in Alpl?/? mice, was present in the generally unmineralized Alpl?/? mantle dentin at ruptured mineralizing matrix vesicles, as detected by immunohistochemistry and by immunogold labeling. However, ablating the OPN‐encoding Spp1 gene in Alpl?/? mice was insufficient to rescue the dentin mineralization defect. Administration of bioengineered mineral‐targeting human TNAP (ENB‐0040) to Alpl?/? mice corrected defective dentin mineralization in the molar roots. These studies reveal that TNAP participates in root dentin formation and confirm that reduction of PPi during dentinogenesis is necessary for odontoblast differentiation, dentin matrix secretion, and mineralization. Furthermore, these results elucidate developmental mechanisms underlying dentin pathology in HPP patients, and begin to explain the reported variability in the dentin/pulp complex pathology in these patients. © 2013 American Society for Bone and Mineral Research 相似文献