Enhanced antitumor efficacy by combination treatment with a human umbilical vein endothelial cell vaccine and a tumor cell lysate-based vaccine

Angiogenesis inhibitors combined with other anticancer drugs have been shown to inhibit tumor growth in animal models and some of them were recently used in clinical trials. In the present study, a whole hepatocellular carcinoma cell lysate based vaccine with diphtheria toxin (DT) and two tandem repeats of microbial HSP70 peptide epitope 407-426 (2 mHSP70407-426, M₂) as adjuvant, which was called HDM, was combined with a whole human umbilical vein endothelial cell (HUVEC) vaccine to develop a combination treatment regimen. This combination treatment regimen was named HUVEC-HDM, which was supposed to enhance its antitumor efficiency. HUVEC-HDM was administrated subcutaneously in both prophylactic and therapeutic procedures. Compared to either single vaccine, HUVEC-HDM induced a more significant inhibition on the growth and metastasis of H22 hepatocellular carcinoma in mice and prolonged the survival of tumor-bearing mice. Besides, HUVEC-HDM immunization elicited strong humoral and cellular immune responses targeting tumor cell as well as tumor angiogenesis, which could be responsible for the enhanced antitumor effect. Moreover, histochemistry analysis showed that HUVEC-HDM induced large areas of continuous necrosis within tumors, correlating well with the extent of tumor inhibition. These results not only highlight the superiority of the combined HUVEC-HDM treatment regimen, but also support the translation of such approaches into the clinic for the treatment of patients with hepatocellular carcinoma.     

暴露法在新生儿脐部护理中的应用

目的 探讨风险预测评估暴露法在新生儿脐部应用的效果.方法 采用随机分组方法将2012年7月1日至2014年1月31日在本院出生的1000例足月新生儿按双盲随机分为实验组和对照组,实验组采用风险评估暴露法对新生儿脐部的影响,评估：①新生儿营养状态和生产过程对新生儿的影响及脐部的情况;②断脐后,暴露其部位可能带来不良的影响;③家属与产妇对暴露脐部的方法接受程度等.脐部暴露方法的处理：用脐带夹结扎脐带包扎24 h后暴露脐带至脐带脱落,这期间每天用75％酒精消毒脐部一次.对照组采用传统的脐部包扎法.观察两组新生儿脐带脱落天数、脐部愈合时间、脐部红肿、出血、分泌物、新生儿呼吸改变等情况.结果 实验组脐部红肿1例（0.20％）、脐部出血2例（0.40％）、脐炎12例（2.42％）、脐带脱落时间（6.59±1.85）d、脐部愈合时间（2.14±0.59）d,对照组脐部红肿12例（2.41％）、脐部出血11例（2.21％）、脐炎80例（16.06％）、脐带脱落时间（7.52±2.21）d、脐部愈合时间（2.35±0.65）d、呼吸改变5例（1.00％）,两组比较差异有统计学意义（P＜0.05）.结论 脐部暴露法应用于新生儿可促进脐带脱落及脐部愈合,减少脐部感染的发生,是安全、有效的新生儿脐部护理方法,值得临床推广应用.     

Dandelion root extract protects NCM460 colonic cells and relieves experimental mouse colitis

The protective potential of dandelion on acute hepatitis, lung injury and colorectal cancer has recently been revealed. Importantly, ulcerative colitis (UC), a clinically defined inflammatory bowel disease, accelerates the risk of colorectal cancer. However, studies focusing on the activity of dandelion on UC are extremely limited. In the present study, we found that an aqueous extract of dandelion root increases cell viability and decreases apoptosis in dextran sodium sulfate (DSS)-incubated NCM460 human colonic epithelial cells, probably through removing the production of reaction oxygen species and blocking nuclear factor-kappaB signaling. We then examined the anti-colitis efficacy of this extract in an in vivo study. We detected that dandelion root extract efficiently ameliorates progressive acute injury as demonstrated by a reduction in body weight loss, severity scores of disease index and shortened colon length during DSS treatment, as well as reducing the inflammatory conditions and oxidative stress in the colon of DSS-induced mice. Our study clearly demonstrates that dandelion has a strong cytoprotective effect on NCM460 colonocytes and shows powerful defense on an established experimental mouse model of DSS-induced UC. Therefore, dandelion root extract can be an effective anti-colitis complex mixture and can provide a complementary alternative to currently available therapeutic intervention in UC.     

sRNA Antitoxins: More than One Way to Repress a Toxin

Bacterial toxin-antitoxin loci consist of two genes: one encodes a potentially toxic protein, and the second, an antitoxin to repress its function or expression. The antitoxin can either be an RNA or a protein. For type I and type III loci, the antitoxins are RNAs; however, they have very different modes of action. Type I antitoxins repress toxin protein expression through interacting with the toxin mRNA, thereby targeting the mRNA for degradation or preventing its translation or both; type III antitoxins directly bind to the toxin protein, sequestering it. Along with these two very different modes of action for the antitoxin, there are differences in the functions of the toxin proteins and the mobility of these loci between species. Within this review, we discuss the major differences as to how the RNAs repress toxin activity, the potential consequences for utilizing different regulatory strategies, as well as the confirmed and potential biological roles for these loci across bacterial species.     

呼吸机质量控制的应用与分析

目的：通过对呼吸机进行质量控制，了解医院在用呼吸机的性能状况及存在问题，以便对呼吸机进行预防性维护和保养。方法：依照总后卫生部颁发的呼吸机质量检测技术规范，采用々业的检测设备，对呼吸机的潮气量、吸呼比、氧浓度、压力参数等各项指标进行检测。结果：探讨呼吸机使用过程中存在的问题及解决方法，指标不合格的呼吸机与设备本身使用年限有密切关系。结论：对呼吸机进行必要的质量控制，提高呼吸机的整体性能，有效防范医疗器械不良事件的重复发生。     

射频消融治疗功能性孤立肾肾细胞癌患者的护理

对11例功能性孤立肾肾细胞癌患者采取射频消融治疗,术后恢复顺利,随访未见复发.提出个性化的心理护理、精心的术前准备,以及术后密切观察有无出血、输尿管狭窄、肾功能情况,做好临近组织损伤、发热的预防护理和出院指导,对患者顺利康复及生活质量的提高有良好的促进作用.