重组2型腺相关病毒-转化生长因子β3对实验性肝纤维化大鼠肝脏组织病理学和Ⅰ型胶原表达的影响

Objective To investgate the effects of TGF β 3 on rat hepatic fibrosis. Methods The TGF β 3 cDNA was cloned into rAAV2 vector. Rats were randomly divided into four groups: normal control group, model group, negative control group and TGF β 3 group. Hepatic fibrosis was induced by hypodermic injection of 40% CCI4. Recombinant AAV2-TGF β 3 viral particles were injected via the vena caudalis one week before CCh treatment. Rats were executed 8 weeks after CCI4 treatment, global histological change was observed after HE staining, the distribution of the collagen fibers was observed after masson staining, his-tochemistry was done to observe the expression of collagen Ⅰ; The positive area rate of the collagen fibers and the average optical rate of collagen Ⅰ were quantified. Results HE staining indicated that collagen fibers were reduced in the TGF β 3 group. Masson staining shown that the collagen fibers were distributed around the blood vessel, in the portal area and disse space. Compared to the model group (13.2%±2.2%) and negative control group (12.3%±1.5%), the collagen fibers in liver tissues of TGF β3 group (7.7% ± 1.5%) were significantly decreased (q = 9.456, P < 0.01; q = 8.217, P < 0.01). Histochemistry indicated that the collagen fibers of liver tissues of TGF 15 3 group (0.185±0.033) were significantly higher than those in the model group (0.252±0.042) and the negative control group (0.230±0.029), (q = 6.228, P < 0.01; q = 4.346, P < 0.01). Conclusion TGF β 3 alleviates the damage to hepatic cell and the level offibrosis in CCI4 treated rats and inhibits the expression of collagen Ⅰ.     

卡文在老年人围手术期应用的初步观察

胃肠外营养自20世纪70年代以来广泛应用于临床，它是通过静脉途径供给患者所需的营养物质，经历了从营养素分瓶输注到倡导混合输注的过程。临床在输注方式上目前仍以分瓶输注为多，但这并不合理，以往的三升袋是将氨基酸、葡萄糖、脂肪酸等营养制剂在配制室混合后输注，由于其营养输入的合理性而受到营养专家多年倡导，但在实际应用上并不如分瓶输注普遍，主要是其存在诸多不便，如渗透压高、保存时间短、要有专用的配制室、输注时间长等，另外在混合输注过程中出现患者不适的比例也较高。     

漏斗胸闭合修补术对患儿心肺功能影响的中期评估

背景/目的:自从引入漏斗胸闭合修补术后,越来越多的患者愿意接受该手术治疗。然而对于该手术对心肺功能的长期影响一直存在争论。本研究旨在对闭合修补术对漏斗胸患儿的肺功能、心功能、运动耐量,以及患儿对其胸部外观的认可和主观运动能力的长期影响进行观察评估。方法:对所有接受漏斗胸闭合修补术的患儿进行前瞻性评估。患儿接受的检查包括术前CT检查、肺功能检查、运动耐量检测以及评估心功能的ECG。在植入支杠后3个月和21个月以及取出支杠后3个月时分别对患儿进行检查。结果:共有48例患儿有术前和术后检查资料,其中有11例在支杠取出后…     

以FAM方案联合化疗治疗恶性肿瘤73例疗效观察

<正> 我院自1990年以来,采用以FAM方案,F(氟尿嘧啶),A(阿霉素或表阿霉素),M(丝裂霉素)为主联合化疗,同时加用其他抗肿瘤制剂治疗各种中晚期恶性肿瘤,有效缓解了病情,延长病人生存期,现将临床疗效观察结果报告如下。     

蒙医药治疗慢性活动性乙型肝炎33例

笔者从一九八七年至一九九一年十二月底以蒙医辨证论治结合蒙医传统疗术导泻、放血疗法为主治疗慢性活动性乙型肝炎33例疗效满意,现报告如下。 一般资料 病例33例,其中:男19例,女14例;病程最长5年,最短1年;年龄最小19岁,最大51岁。主要症状以两胁或右胁胀痛、胸闷、腹胀、食欲不振、肝掌、蜘蛛痣、肝脾肿大、舌淡或暗红、苔厚白或薄白等。化验室检查:HBSAg阳性24例,抗—HBC阳性28例,HBeAg阳性16例,肝功能异常26例。     

MEDICA钾钠氯分析仪检修一例

1故障现象 仪器校标后不能进入分析血样状态. 2分析与检修     

022 淡黄色巴豆叶精油的成分分析及其抗癌活性

淡黄色巴豆（拟）Croton flavens L．是加勒比海地区特有植物，作者研究了该植物叶精油的化学成分并进行了细胞毒试验。