锂盐对大鼠行为和间脑递质含量的影响

本文观察锂盐对大鼠行为、体温、心率及间脑递质含量的影响:方法:分别给体重为180～220克的成年大鼠(雌雄兼用)腹腔注射锂盐,第一组10mg/100g/天(n=7),第二组20mg/100g/天(n=8),各连续3天。第三组10mg/100g/天(n=7),连续5天。以等量的生理盐水作对照(n=6),用 RF-510型荧光分光光度仪,测定间脑单胺类递质含量。     

羟氯喹对女性头面部盘状红斑狼疮与亚急性皮肤型红斑狼疮皮损疗效比较

皮肤红斑狼疮(cutaneous lupus erythematosus,CLE)是红斑狼疮中一种累及皮肤而系统表现相对较轻的类型,以育龄期妇女发病为主[1]。在CLE病谱中,以盘状红斑狼疮(discoid lupus erythematosus,DLE)及亚急性皮肤型红斑狼疮(subacute cutaneous lupus erythematosus,SCLE)尤为常见[2]。抗疟药、维A酸类药物、沙利度胺、氨苯砜等均为CLE系统性治疗中的常用药物,但维A酸类和沙利度胺类都有较强的致畸性,使得其在具有生育要求的女性患者中使用有一定     

126株生殖器部位念珠菌对三唑类药物的敏感性分析

目的:了解生殖器部位念珠菌感染的菌型、分布及其对三唑类药物的敏感性,指导临床合理使用抗真菌药。方法:对2015年1月-2016年9月培养出的126株念珠菌,用ATBID32C酵母菌鉴定反应板鉴定菌种,用Rosco纸片扩散法进行体外药敏检测,比较不同菌型念珠菌的药敏趋势。结果:126例生殖器部位念珠菌感染者中,白念珠菌93例(73.81%)、近平滑念珠菌13例(10.32%)、平滑念珠菌9例(7.14%)、无名念珠菌4例(3.17%)、其他念珠菌7例(5.56%);念珠菌检出构成比以30-39岁最多;女性多于男性。药敏结果分析发现,白念珠菌中74.19%对氟康唑敏感,而仅有45.16%对伊曲康唑敏感,敏感率差异具有统计学意义(χ~2=16.29,P0.01);非白念株菌中60.61%对氟康唑敏感51.52%对伊曲康唑敏感,差异无统计学意义(χ~2=0.55,P=0.457)。结论:生殖器部位念珠菌感染以白念珠菌为主,其次为近平滑念珠菌。白念珠菌对氟康唑的敏感率较伊曲康唑高,非白念珠菌对两种药物的敏感率无明显差别。     

茶多酚诱导的Nrf2通路和自噬在痤疮发病机制中的作用

痤疮是一种常见的毛囊皮脂腺的慢性炎症性疾病,主要累及表皮和毛囊皮脂腺单位,引起局部皮肤炎性或非炎性病变。其发病机制主要包括雄激素刺激皮脂分泌亢进、皮脂排泄受阻、毛囊内痤疮丙酸杆菌的定植及炎性反应。茶多酚,作为一种Nrf2激活剂,其有效成分没食子儿茶素没食子酸酯可激活Nrf2信号通路或自噬,通过抑制痤疮丙酸杆菌的增殖、抑制炎症的蔓延、减少皮脂的分泌等途径治疗痤疮。深入研究Nrf2信号通路和自噬在痤疮发病机制中的关系和作用,有助于为临床治疗痤疮开辟一个新方向。     

试论精血不足与皮肤内源性衰老的相关性*

皮肤老化程度是评价人体衰老程度最重要的指标之一，而针对皮肤老化的中西医病因的探讨，对于治疗皮肤老化性疾病以及预防推迟甚至逆转皮肤老化具有重要的临床意义。本文从皮肤老化的现代病因、中西医病因病机着手，结合中医理论及自身临床经验，对中医精血不足与皮肤内源性衰老的相关性进行了论述，为临床应用补精活血方法治疗皮肤老化性疾病提供一定的理论与临床依据。     

Synergistic effects of tetrandrine on the antifungal activity of topical ketoconazole cream in the treatment of dermatophytoses: A clinical trial

Objective:To evaluate the synergistic effects of tetrandrine(TET)on the antifungal activity of topical ketoconazole(KCZ)in the treatment of dermatophytoses.Methods:The minimum inhibitory concentrations(MICs)for KCZ and combined KCZ and TET were compared in vitro.A randomized,double-blind trial was conducted among 97 patients with dermatophytoses who were assigned to 3 groups and received: treatment with combination of 2%KZC and 2%TET cream(KCZ+TET group),or only 2%KZC cream(KCZ group),or 2%TET cream(TET group).Patients with tinea corporis and/or tinea cruris were treated for 2 weeks, separately.The patients with tinea pedis and/or tinea manuum were treated for 4 weeks.Results:Compared with KZC alone,combined use of KZC and TET showed lower MICs against clinical isolates of dermatophytes (P<0.05 for all).In the patients with tinea corporis and/or tinea cruris,the rates of overall cure(clinical cure plus mycologic clearance)were 81.25%vs.33.33%for combined treatment and KZC monotherapy,respectively, after 4 weeks.All clinical indices were significantly different between the combination therapy and only KCZ therapy groups(P<0.05).Among the patients with tinea pedis and/or tinea manuum after 4 weeks treatment,the overall cure rates in the KCZ + TET group and KCZ group were 75.00%vs.40.00%,respectively.In the KCZ + TET group,all the clinical indices were significantly better than those in the KCZ group and TET group(P<0.05). The rates of overall efficacy in the TET group were all zero.No local skin redness or itching was observed during TET treatment.No clinically significant changes were found in post-treatment routine blood,urine,or stool tests, ECG,or tests for liver and kidney function;no serious adverse events occurred.Conclusion:TET synergistically enhanced the clinical efficacy of topical KZC cream in the treatment of dermatophytoses.     

miR-214-5p通过DNMT1介导的AXIN2基因DNA甲基化修饰在皮肤基底细胞癌中的作用机制

目的 探讨皮肤基底细胞癌中轴抑制蛋白2(Axis inhibition protein 2,AXIN2)基因启动子甲基化对基因转录的影响及miR-214-5p通过靶向DNA甲基转移酶1(DNA methyltransferase1,DNMT1)对AXIN2甲基化率的调控机制。方法 收集2022年1月-2023年6月在广州市皮肤病防治所就诊治疗的102例皮肤基底细胞癌(Cutaneous basal cell carcinoma, BCC)患者作为研究对象,提取癌组织和癌旁正常组织标本及基线资料。焦磷酸测序法检测AXIN2基因启动子区甲基化率。实时荧光定量PCR检测AXIN2、DNMT1基因mRNA和miR-214-5p的表达水平。将miR-214-5p模拟物(mimic)、抑制物(inhibitor)及其阴性对照(mimic NC和inhibitor NC)分别对基底细胞癌A431细胞进行转染,48 h后检测DNMT1基因mRNA表达水平和AXIN2基因甲基化率。结果 BCC癌组织的AXIN2基因甲基化率显著高于癌旁正常组织(t=5.128,P<0.001),AXIN2基因mRN...     

姜黄素和茶多酚对不同时间UVB致无毛鼠皮肤急性光损伤的防御作用

目的 探讨姜黄素和茶多酚对不同时间UVB致无毛鼠急性光损伤的防御作用。方法 取清洁级无毛鼠36只, 随机分为对照组及药物组(0 s、30 s、60 s、120 s、240 s);每组各2只。于照射前30 min在无毛鼠背部涂抹姜黄素或茶多酚, 照射距离为15 cm, 采用3倍最小红斑量(MED)的UVB约200～540 mJ/cm²分别照射各组, 取皮肤组织, 制成石蜡切片, 光学显微镜观察。结果 对照组无毛鼠皮肤出现不同程度鳞屑, 胶原间可见散在淋巴细胞, 且120 s及240 s时出现胶原红染、排列紊乱、均质化等改变。药物组无毛鼠真皮中炎性细胞浸润减少, 胶原受损现象得到改善。结论 外用姜黄素和茶多酚可通过减轻炎症细胞浸润及减轻胶原受损程度而防御UVB致无毛鼠皮肤急性光损伤。     

IL-33和IL1RL1基因单核苷酸多态性与特应性皮炎相关性研究

目的：分析IL-33和IL1RL1基因单核苷酸多态性(SNPs)与特应性皮炎（AD）的相关性。方法：PCR-SSP方法检测89例AD患者和138名正常对照组受试者IL-33和IL1RL1编码基因的等位基因和基因型频率。结果：AD患者的IL-33 rs1800925 C等位基因的频率高于对照组，而T等位基因和rs764706552等位基因G低于对照组（均P＜0.001）。IL-33 rs1800925基因型CC和rs764706552基因型CG与AD显著正相关（均P＜0.001）。两组患者的IL1RL1基因的等位基因和基因型频率无明显差异（均P＞0.05）。结论：IL-33基因可能与AD的发病相关。