不同持续时间的周期性压力对构建组织工程软骨的影响

Objective To determine the best duration for exerting the cyclic pressure under which the tissue-engineered cartilage is constructed. Methods Free chondrocytes isolated from rabbit articular cartilage were seeded into polylactic acid-co-glycolic acid(PLGA) scaffolds after expansion in vitro, and ran-domized into 4 groups. In Groups 1 to 3, chondrocytes were cultured under daily cyclic pressure (0 ～ 200 kPa, 0.1Hz) for 4 hours, 8 hours, 12 hours respectively; Group 4 was a control in which no pressure was exerted. In each group, after 2 weeks of culture, the tissue engineered cartilages were observed in vitro and assessed by his-tological staining of liE. Next, the content of DNA and the secretion of type Ⅱ collagen and GAG in cartilages were detected quantitatively. Results Under the daily cyclic pressure (0 ～200 kPa, 0.1 Hz), the scaf-fold-chondrocytes complex in the group of 8 hours got the largest volume, smooth, lucidus, and elastic surface, the most queuing chondrocytes, and the highest content of type Ⅱ collagen and GAG (P < 0.01). Conclusions Since chondrecytes are baro-senstive, the metabolism of chondrocytes can be affected by the time of cyclic pressure. Under the effect of 0 ～200 kPa, 0.1Hz, the daily cyclic pressure of 8 hours may be optimal for chondrocytes to multiply and synthesize extracellular matrixes such as type Ⅱ collagen and GAG.     

中西医结合保守治疗异位妊娠85例

目的:观察氨甲蝶呤(MTX)及中草药联合保守治疗异位妊娠的疗效。方法:对85例异位妊娠的患者随机分组,分别应用MTX及中草药联合治疗异位妊娠52例,治愈50例,总有效率96.15％;MTX治疗33例,治愈25例,总有效率75.76％,两者差异有显著性(P<0.05);而远期效果无显著性差别(P>0.05)。结论:MTX及中草药联合治疗近期效果明显优于单用MTX,远期效果无显著性差别。说明MTX及中草药联合治疗异位妊娠是一种高效、安全的方法。     

恶性肿瘤在治疗过程中并发的伪膜性肠炎的护理

伪膜性肠炎是金黄色葡萄球菌所致的急性肠道疾病 ,临床上常见于应用广谱抗菌素的患者 ,也是化疗过程中一种极为严重的并发症 ,死亡率很高。我院曾遇到过 1例化疗并发伪膜性肠炎、麻痹性肠梗阻的患者 ,经治疗痊愈。现将本例临床情况及护理经过简要介绍如下。病例简介 :女 ,38岁。因不规则阴道流血 4 0余天 ,气急 3ｄ而急诊入院。全身情况很差 ,急性重病容 ,呼吸急促。查体 :子宫孕 10周大 ,双侧附件均增厚 ,左侧有一鸭蛋大小之包块。胸片显示双肺布满阴影。妊娠试验 1 5 0阳性。诊断为子宫绒毛膜上皮癌 ,双肺广泛转移 ,盆腔转移。入院第2ｄ开…     

α-干扰素中枢镇痛功能及其结构基础

神经系统和免疫系统之间有着广泛的联系,细胞因子在其中起着重要作用.细胞因子被证实有免疫调节和神经、内分泌等多种生物学效应,具有多个功能位点和结构域,并且可以与不同受体或受体亚型相结合,产生相应的生物效应[1～3].α-干扰素(IFNα)是研究最早的、也是研究最多的作用于神经系统的免疫细胞因子之一,其中枢镇痛功能及其结构基础是近年来研究的热点.本文就国内外对这方面的研究综述如下.     

溃疡病外科治疗现状与展望

溃疡病外科治疗现状与展望高峰，董国光，王金亭（附属第一医院普外科）石颖（义县人民医院外科）胃、十二指肠溃疡是一种常见病，约１５～２０％的患者需手术治疗。自Ｂｉｒｌｌｏｒｔｈ胃大部切除术问世以来，已有百余年的历史，它是通过切除胃泌酸区、减少胃酸分泌和切...     

血管活性肠肽与结肠癌

血管活性肠肽与结肠癌高峰张胜本张连阳第三军医大学大坪医院野战外科研究所普外科四川省重庆市６３００４２ＳｕｂｊｅｃｔｈｅａｄｉｎｇｓＶａｓｏａｃｔｉｖｅｉｎｔｅｓｔｉｎａｌｐｅｐｔｉｄｅＲｅｃｅｐｔｏｒｓ，ｖａｓｏａｃｔｉｖｅｉｎｔｅｓｔｉｎａｌ...     

宫乐散治疗宫颈糜烂的临床研究

宫颈糜烂是生育年龄妇女的常见病,多发病之一,发病率高达70-80％,我们采用自行配制宫乐散(以消炎生肌为主)的方法随机抽样治疗宫颈糜烂200例,取得了满意的效果。     

高血压性脑出血外科治疗体会

报告33例高血压性脑出血施行外科手术治疗的临床体会。结合术前病情分级、血肿类型、血肿量、中线结构移位程度等,对手术治疗的适应症选择、手术方式及入路、提高治疗效果、减少术后并发症等作了讨论。提出了手术治疗重要性。     

不同持续时间的周期性压力对构建组织工程软骨的影响

Objective To determine the best duration for exerting the cyclic pressure under which the tissue-engineered cartilage is constructed. Methods Free chondrocytes isolated from rabbit articular cartilage were seeded into polylactic acid-co-glycolic acid(PLGA) scaffolds after expansion in vitro, and ran-domized into 4 groups. In Groups 1 to 3, chondrocytes were cultured under daily cyclic pressure (0 ～ 200 kPa, 0.1Hz) for 4 hours, 8 hours, 12 hours respectively; Group 4 was a control in which no pressure was exerted. In each group, after 2 weeks of culture, the tissue engineered cartilages were observed in vitro and assessed by his-tological staining of liE. Next, the content of DNA and the secretion of type Ⅱ collagen and GAG in cartilages were detected quantitatively. Results Under the daily cyclic pressure (0 ～200 kPa, 0.1 Hz), the scaf-fold-chondrocytes complex in the group of 8 hours got the largest volume, smooth, lucidus, and elastic surface, the most queuing chondrocytes, and the highest content of type Ⅱ collagen and GAG (P < 0.01). Conclusions Since chondrecytes are baro-senstive, the metabolism of chondrocytes can be affected by the time of cyclic pressure. Under the effect of 0 ～200 kPa, 0.1Hz, the daily cyclic pressure of 8 hours may be optimal for chondrocytes to multiply and synthesize extracellular matrixes such as type Ⅱ collagen and GAG.     

肝门部胆管成形空肠袢皮下埋置术治疗肝胆管结石的临床观察

为探讨肝门部胆管成形空肠袢皮下埋置术治疗肝胆管结石的效果，笔者回顾性分析近5年来采用肝门部胆管成形空肠袢皮下埋置术治疗23例肝胆管结石患者的临床资料。全部病例手术成功;对2例结石复发患者利用纤维胆道镜通过皮下埋置的空肠袢取石后治愈。本组结果提示本术式系一种理想的治疗肝胆管结石的方法，能为手术后结石复发的治疗提供一条方便快捷的通道。