中国苏芸金芽孢杆菌肯尼亚亚种Ag株发酵工艺条件研究

 目的 开发具有特异性杀虫谱特性的Bt菌新株系.方法 在对摇瓶培养研究的基础上,通过改变培养基成分、通气量和pH等操作条件,检测不同条件对菌体生长量、芽孢及伴孢晶体形成的影响,研究中国苏芸金芽孢杆菌肯尼亚亚种Ag株(Bt Ken-Ag)发酵罐间歇发酵的工艺条件.结果 中国Bt Ken-Ag发酵罐发酵最佳工艺条件:发酵温度维持30℃,发酵前期,控制pH为7.0,用较大的通气量1:2.0 V/(V·min);发酵后期不控制pH,并适当减小通气量为1:0.5V/(V·min).发酵前期菌体达到7.5×10<'10>/ml的较高浓度,发酵后期菌体向芽孢转化的转化率高达98%以上.结论 初步得出适于该菌间歇发酵的最佳工艺,为该菌制剂的应用提供可靠依据.     

加巴喷丁胶囊治疗疱疹后神经痛的多中心临床观察

Objective To observe the clinical efficacy and safety of gahapentin in the treatment of postherpetic neuralgia. Methods A multicenter, randomized, double-blind, placebo-controlled, parallel design, 6-week study was performed. Patients with postherpetic neuralgia were recruited into this study and randomly divided into two groups to receive gabapentin or placebo 1800 mg daily in three divided doses with a forced titration schedule, respectively. The primary efficacy measure was change in the pain score based on a visual analogue scale from baseline to the final week of therapy, and secondary measure was the improvement in sleep quality scored on a 5-point severity scale. Efficacy and safety evaluation was performed at baseline, and 1, 3, and 6 weeks atter the treatment. Results One hundred and forty-one patients were recruited in four clinical centers, and 125 patients completed the trial, of whom 66 were in the treatment group and 59 in the control group. An improvement was observed in both pain scores and sleep scores on week 1, 3 and 6 in both two groups, and the improvement was greater in gabapentin-treated group than that in the control group. The response rate was 29.58% and 57.75%, respectively in gabapentin-treated group on week 1 and 3, com-pared to 13.04% and 40.58%, respectively, in the control group (t = 5.94, 4.12, respectively, both P ＜0.05).Gabapentin was well tolerated, and the most common adverse events were dizziness, vertigo, somnolence and transient abnormality of hepatic function. Conclusion Gabapentin could markedly reduce pain intensity and improve sleep quality with a low incidence of adverse events in patients with postherpetic neuralgia.     

Effects of α-melanocyte-stimulating hormone on the production of TNF-α and IL-10 by peripheral blood mononuclear cells from patients with psoriasis

Objective To investigate the effects of alpha-melanocyte-stimulating hormone (alpha-MSH) on the production of tumor necrosis factor-alpha (TNF-alpha) and intedeukin-10 (IL-10) by peri-pheral blood monohuclear cells (PBMCs) from patients with psoriasis vulgaris. Methods Heparinized peri-pheral blood was obtained from 20 patients with psoriasis vulgaris and 10 healthy human controls. PBMCs were isolated, cultured in complete medium, and stimulated with phytohemagglutinin (PHA) alone, the com-bination of PHA and various concentrations of alpha-MSH, or nothing. After another 48-hour culture, ELISA and real-time PCR were performed to measure the secretion levels of TNF-alpha and IL-10 in the super-natants of cultured PBMCs as well as the mRNA expression levels of TNF-alpha and IL-10 in PBMCs. Results The secretion level of TNF-alpha in the supematants of patient-derived PBMCs stimulated by nothing or PHA alone was significantly higher than that from normal control-derived PBMCs (329.87 ± 99.33 ng/L vs 116.95 ± 37.15 ng/L, 1756.01 ± 183.60 ng/L vs 1287.30 ± 152.36 ng/L, both P<0.01). alpha-MSH of all tested concentrations (10-13, 10-11, 10-7,mol/L) could inhibit the secretion of TNF-alpha by PBMCs com-pared with PHA alone (all P < 0.01), and the maximum effective concentration was 10-13 mol/L. On the con-Wary, a significant decrease was observed in the secretion level of IL-10 in the supematants of patient-derived PBMCs stimulated by nothing or PHA alone compared with normal control-derived PBMCs (P <0.05 or 0.01). Moreover, the secretion of IL-10 by PBMCs was promoted by alpha-MSH of all tested con-centrations (P < 0.01 or 0.05), with the maximum effective concentration being 10-13 mol/L (P < 0.01). The alpha-MSH of 10-13 mol/L down-regulated the mRNA expression of TNF-alpha (P < 0.001), but up-regnlated that of IL-10 (P < 0.001) in PHA-stimulated PBMCs from patients. Conclusion alpha-MSH can regulate the production of TNF-alpha and IL-10 by PHA-stimulated PBMCs from patients with psoriasis vulgaris.     

超声引导经皮脾内注射鱼肝油酸钠治疗肝硬变性脾功能亢进13例

1材料和方法 1.1材料男11例,女2例,年龄14岁～61岁,肝功能A级4例,B级6例,C级3例.都有食管静脉曲张.脾脏肋下3 cm～10 cm.常有出血症状、体征18例次.血小板(Pt)数(21～64)×109/L,白细胞(WBC)计数(2.4～3.4)×109/L,血红蛋白(Hb)70 g/L～135 g/L,骨髓穿刺检查排除了血液病,且骨髓中出现相应的外周血细胞系的幼稚细胞过度增生.     

钆塞酸二钠MR增强成像与CT增强成像诊断肝脏局灶性病变的对照研究

目的 对比钆塞酸二钠MR增强成像与CT增强成像在诊断肝脏局限性病变中的诊断价值。方法 纳入我院2015至2021年术前进行钆塞酸二钠MR增强成像和CT增强成像且具有术后病理诊断结果 的患者66例。以术后病理结果 为标准,对比钆塞酸二钠M R增强成像与CT增强成像对肝脏局灶性病变的诊断价值及准确性。结果 66例患者中,有29例肝细胞肝癌、15例胆管细胞癌、12肝脏血管瘤、3例肝腺瘤、4例肝脏局灶性增生、3例肝脓肿。CT增强成像诊断的准确性分别为83%、80%、75%、67%、50%、67%,钆塞酸二钠MR增强成像诊断诊断的准确性分别为97%、100%、92%、67%、100%、100%。钆塞酸二钠MR增强成像的对肝脏局灶性病变诊断的准确率高于CT增强成像,差异具有统计学意义(P=0.005)。结论 钆塞酸二钠MR增强成像对肝脏局灶性病变具有更高的诊断价值,优于CT增强成像。     

上海地区汉族健康人群PAF乙酰水解酶基因4号外显子275位点G/A单核苷酸多态性

目的了解血小板活化因子乙酰水解酶(PAF-AH)基因4号外显子275位点G/A位点单核苷酸多态性(SNP)在中国上海地区汉族健康人群中的分布及与其他种族比较的特点。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对100名上海地区汉族健康者PAF-AH4号外显子275位点G/A位点的SNP进行了检测,计算其基因型和等位基因频率,并结合文献进行了不同种族间的分析比较。结果上海地区汉族健康人群GG基因型频率最高(88%),GA基因型次之(12%),未发现AA基因型。与英国人相比,上海地区汉族人群G等位基因频率较高。结论上海地区汉族健康人群G→A位点碱基突变率较低,其SNP与英国人相比分布存在明显差异。     

艾滋病合并隐球菌性脑膜炎1例

患者男,42岁。因发热17d,头痛,呕吐10d,于2004年8月11日在我院神经科住院治疗,患者于2004年7月23日外出旅游后出现咽痛,咳嗽,发热,体温38.5℃,7月28日在我院急诊科就诊给予抗生素及对症处理,咽痛,咳嗽好转,但出现头胀痛,以双侧颞部为重,并出现2次非喷射性呕吐,未予以特殊处理。8月5日头痛,呕吐加重,出现烦躁,不能行走,     

利用基因芯片筛选与银屑病发病相关的GPCR家族基因

目的从基因水平上探讨G蛋白耦联受体(GPCRs)在银屑病发病机制中的作用,讨论通过抑制GPCRs的方法治疗银屑病的可能性,为后期寻找银屑病的新的治疗靶点提供依据。方法利用基因芯片检测技术和实时定量PCR的方法找出3例斑块型银屑病患者受累皮肤组织和非受累皮肤组织与GPCRs相关的大于2倍差异基因。结果通过基因芯片技术和实时定量PCR验证得出6个上调基因(CXCR6,GPR110,GPR171,CXCR2,PTARF,LPHN2)和11个下调基因(GPR12,CHRM1,ADRB2,CHAM3,NPY1R,ADRA1B,GPR182,ADAR2A,LGR6,GPRC5C,F2R)。其中,CXCR6已被证实与银屑病的发病相关。结论银屑病患者受累皮肤组织和非受累皮肤组织与GPCRs相关的差异表达基因与银屑病的发病机制密切相关,值得进一步深入探讨。     

疱疹样天疱疮合并晚期宫颈癌1例

患者,女,50岁。躯干红斑、脓疱,伴剧痒1个月。组织病理检查示:角层下水疱,棘细胞海绵水肿,中性粒细胞及嗜酸粒细胞浸润。直接免疫荧光示:棘细胞间IgG沉积。患者3个月前被诊断为晚期宫颈癌。     

FoxM1在寻常性银屑病患者皮损中的表达

目的研究寻常性银屑病患者皮损中Fox M1(forkhead box M1)表达情况。方法寻常性银屑病及正常对照标本各30例,采用En Vision二步法进行免疫组化染色。结果 Fox M1在正常皮肤组织中表达减低,在寻常性银屑病皮损染色强度显著增高(Z=-4.965,P=0.000,P0.05)。结论 Fox M1可能与银屑病皮损角质形成细胞过度增生相关。