Differentiation of human epidermal stem cells into fibroblasts induced by TGF-β1 in vitro

Objective To investigate the correlation between human epidermal stem cell (hESCs) and hypertrophic scar or keloid. Methods Improved collagen Ⅳ-coated adhesion methods was used to isolate and culture the epidermal stem cells after neutral protease selectively digested the dermo-epidermal junctions. After the cells were cultured and expanded in vitro, and passage 3 hESCs were induced by different concentrations of TGF-β1 (0.1, 5.0, and 10.0 ng/ml). Morphological fea-tures and identification of these cells were meseasured by HE, Masson, immunohistochemical staining on the days 3 and 7, respectively. Results After induced by TGF-β1 for 3 and 7 days, the morpholo-gy of the epidermal stem cell (hESCs) was changed into fusiform shape, similar to fibroblasts. 70 % ofthe cell which was induced by TGF-β1 were blue stained in the cytoplasm by Masson stain, which is the distinctive method for collagen, suggesting collagen appeared or increased in the cells. The collagen concentrations in supernatants of hESCs were 0.4150±0.0014, 0.3380±0. 0020, and 0.3870±0.0020, much higher than that in control group (0.0780±0.0025) and normal skin fibro-blast group (0.15004±0.0051) (P<0.05). Immunohistochemical staining revealed that positive rates of these cells for anti-vimentin staining were more than (95.00±1.20)% in experiments and (5.70±0.20)% in control group. Conclusion The differentiantion of hESCs induced by TGF-β1 into fibro-blasts indicates that hESCs may play a role in the pathogenesis of hypetrophic scar and keloid.     

PCR技术在尖锐湿疣诊断和鉴别诊断中的应用

ＰＣＲ技术在尖锐湿疣诊断和鉴别诊断中的应用范治璐，刘志宇，徐志强，董德刚，聂大平，韩锐关键词探针，核酸，原位杂交，尖锐湿疣中图法分类号Ｒ７５２．５３乳头状瘤病毒广泛存在于自然界，可感染人及许多脊椎动物，而人类乳头状瘤病毒感染对象只是人。本文门诊诊断为...     

小儿腹内恶性淋巴瘤九例报告

小儿腹内恶性淋巴瘤临床上比较少见,早期诊断十分困难,目前尚无特异的检查疗法。临床上主要靠穿刺,剖腹探查,病理检查确诊,预后较差。我院1979～1988年共收治小儿腹内恶性淋巴瘤9例。报告如下。临床资料一、性别、年龄、临床表现本组病例中男7例,女2例,男女之比为3.5:1。年龄3.5～13岁,以4～7岁为最多,共6例。全组病例均有间歇性腹痛,最初腹痛不经治疗可自行缓解,随着病情进展而呈阵发性加剧。从开始腹痛到就诊时间最短17天,最长4个月。腹痛伴腹部包块者5例,腹泻2例,血便2例,黄疸1例。6例     

干槽症的细菌学研究及甲硝唑微栓预防和治疗应用

本文通过对19例拔牙后干槽症患者牙窝内容物的细菌培养和药敏试验,对引起该病的特殊细菌和病因进行了探讨。同时采用甲硝唑微栓置入383例下颌阻生第三磨牙拔牙创内,观察其预防和治疗干槽症的效果,结果表明甲硝唑微栓在预防和治疗干槽症上具有明显效果。     

78结合牙1例

患者张某，女，52岁，因左上后牙自发痛2月余来诊。此牙2年前曾诊治。2月前此牙痛复发，在当地医院诊治至今，目前仍有咬合痛，偶有自发痛，来我科要求拔除，检查：7倾侧远中处银汞充填，近中洞达根底，叩（ ），松动（-），牙龈（-），8未查及，无牙列拥挤，根尖片：7根尖透射影，8阻生，78相连，其间未见牙周膜，诊断：（1）7慢性根尖周炎；（2）78为结合牙（8阻生）。     

湘西土家族、苗族、侗族癫痫流行病学调查

湘西土家族、苗族、侗族癫痫流行病学调查王华，雷花香，黄定梅，邓朝晖多年来，虽然癫痫的流行病学研究较多，但有关土家族、苗族、侗族的癫痫调查资料却极少。作者于１９９２年３月～７月对居住在湘西的这三个民族的人群进行了癫痫的流行病学调查。现将有关资料报告如下...     

Dental Whitening脱色剂漂白氟斑牙的临床疗效观察

氟斑牙是牙体异常矿化着色引起的牙体疾病，严重影响牙齿的发育和面容美观 ,患者心理压力巨大。随着人们生活水平的提高，门诊的求诊量逐年增多。近年来我们采用美国新产品ＤｅｎｔａｌＷｈｉｔｅｎ ｉｎｇ脱色剂对９８例氟斑牙患者进行漂白治疗，效果良好。１资料与方法１．１临床资料（１）患者９８例 ,其中男２０例 ,女７８例 ,年龄１７～４５岁 ,平均年龄２５岁。（２）临床分级 :Ｉ级，氟斑牙呈棕黄色斑纹。Ⅱ级 ,氟斑牙呈棕黄色伴点状釉质发育不全。Ⅲ级 ,氟斑牙呈棕色伴坑状釉质发育不全。（３）材料：美国产ＤｅｎｔａｌＷｈｉｔｅ…     

缺血性心脏病(CHD)30例临床治疗

1998－01～1998－07我们应用生脉注射液治疗CHD30例，取得较好疗效，现将结果报道如下。l资料与方法1．l病例选择。本组60例，均有不同程度的胸闷、心悸、气短症状；心电（ECG）示心肌缺血性改变（ST－T改变）；二维心动超声（＊＊G）示左小室舒张功能降低，随机分成两组。治疗组，男16例，女14例，平均年龄64岁；对照组，男17例，女13例，平均年龄63岁。l．2方法。治疗组：常规疗法加生脉注射液6Oml溶于5％Glmoze或O．85％Natriichloricli25Oml中ivgtt．qd，两周一疗程。对照组：常规疗法，硝酸酯类药物，和／或加用p受体阻滞剂．和…