鼻咽癌31例EB病毒壳抗原免疫球蛋白A抗体的测定(摘要)

本文应用免疫荧光细胞化学方法对西安地区31例鼻咽癌患者、13例非鼻咽癌患者(包括头颈部其它恶性肿瘤6例,鼻咽粘膜慢性炎症7例)进行了VeA-IgA抗体测定。检测结果:31例鼻咽癌中,28例系低分化鳞癌,VcA-IgA抗体阳性者25例(滴度1:10至1:160),占低分化鳞癌的89.28％,高分化鳞癌2例,腺癌1例,该两型癌VcA-IgA抗体均为阴性。13例非鼻咽癌中,头颈部其它恶性肿瘤6例均阴性;鼻咽粘膜慢性炎症5例阴性,2例阳性(滴度1:10)。对照试     

儿童恶性骨肿瘤患者Th1/Th2亚群漂移的研究

儿童恶性骨肿瘤的发病率仅次于造血系统恶性肿瘤,其中成骨肉瘤约占儿童恶性肿瘤的5%。本病对手术及放化疗的效果不佳,预后极差[1]。随着肿瘤免疫学的发展,以细胞因子为核心的免疫治疗逐渐被临床所接受。根据分泌细胞因子的不同,可将辅助性Ｔ淋巴细胞亚群分为辅助性亚群1(Ｔｈ1)和辅助性亚群2(Ｔｈ2)两个亚群。Ｔｈ1亚群与Ｔｈ2亚群平衡状态的失调,必然导致细胞因子网络的紊乱,从而影响细胞免疫和体液免疫的功能。通过检测细胞因子的水平,可以反映Ｔｈ1/Ｔｈ2的平衡状态[2]。恶性骨肿瘤患儿体内Ｔｈ1/Ｔｈ2状态尚未见报道。本实验对此进行了…     

纳米疫苗经不同途径给药后的抗肿瘤免疫学效应比较

目的比较纳米乳剂包裹的复合抗原NE(MHS)经不同途径免疫小鼠后的抗肿瘤免疫学效应,探索肿瘤疫苗有效的给药途径。方法采用磁力超声法制备纳米疫苗NE(MHS)(融合蛋白MAGE1-HSP70和超抗原SEA按摩尔比100∶1混合),评价其粒径、包裹率及稳定性。以NE(MHS)经口服、皮下、静脉和腹腔注射4种途径免疫C57BL/6小鼠,IFN-γELISPOT和LDH杀伤实验检测小鼠特异性细胞免疫反应情况,肿瘤治疗、攻击实验比较4种不同途径免疫的抗肿瘤效应。结果所制备纳米乳剂为乳白色均质胶体混悬液,粒径为20±5nm,包裹率为87%。B16-MAGE1接种后第28天,口服免疫组小鼠肿瘤体积明显大于其他3组(P<0.05),而小鼠脾淋巴细胞中分泌IFN-γ的特异性T淋巴细胞频数及对B16-MAGE1的杀伤效果、荷瘤小鼠的生存期、小鼠成瘤时间等4组间两两比较均无统计学差异。结论纳米乳剂疫苗NE(MHS)经不同途径给药后对小鼠的抗肿瘤免疫学效应相近,表明其适用于多种途径给药(包括口服),是一种使用方便的肿瘤基因工程纳米疫苗。     

131 碘肝癌单克隆抗体Fab片段偶合物体内放射免疫显像研究

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The determination and significance of C/EBP in cells of liver cancer and different liver tissues

C/EBP is a sequence-specific DNA-binding protein.In order to identify its distribu-tion,localization and function,immunocytochemical technique(ABC method)was done usinganti-C/EBP polypeptide antibodies 1103~#,425~# in liver specimens from 20 normal adult,5neonatal,6 patients with hepatitis,25 patients with liver cirrhosis,80 patients with hepatocellu-lar carcinoma(40 cases were associated with surrounding nontumorous tissues)and 26 patientswith cholangiocarcinoma(15 cases were associated with surrounding nontumorous tissues).Theresults showed that C/EBP was diffusely distributed in nuclei and cytoplasm of differentiated liv-er cells and very low or undetectable in liver cancer cells.The expression of C/EBP was in pro-portion to differentiated degree of tumor cells,and was obviously weaker than that in surround-ing nontumorous tissues.C/EBP positive staining has also been found in regenerating epithelialcells of bile ductules.The results suggested that C/EBP might play an important role in estab-lishing and maintaining the differentiation of liver cells and might exert an inhibiting effect againsttransformation of liver cells and proliferation of neoplastic tissue.     

人原发性肝细胞癌中HBxAg的免疫组化及免疫电镜研究

作者用免疫组化和胶体金免疫电镜方法,观察乙型肝炎x抗原(HBxAg)在肝癌组织(35例)及癌旁肝组织(29例)内的分布及亚细胞定位。结果显示,HBxAg在癌组织及癌旁肝组织内的检出率分别为60.0％和82.8％,阳性物质主要位于细胞浆内。电镜观察,HBxAg主要定位于粗面内质网和胞浆基质中,少数位于核内,并可见游离核糖体及核孔附近阳性,推测HBxAg在内质网及游离核糖体上合成,并可经核孔进入核内。     

肝癌免疫导向药物内在化及其意义

肝癌免疫导向药物内在化及其意义隋延仿孙志伟陈志南肝癌免疫导向药物内在化及其意义@隋延仿@孙志伟@陈志南...     

Relationship Between Effect of ~(60)Co Radiotherapy on Nasopharyngeal Carcinoma and EB Virus Capsid Antigen-lmmunoglobulin A Antibody Level

By using immunofluorescent cytochemical method,EB-VCA-IgA antibody levelswere studied in 35 cases of nasopharyngeal carcinoma.It was found that VCA-IgAantibody level declined with the improvement of the patient's condition and rose up withthe worsening of the disease in most cases.It is indicated that the change of EB-VCA-IgAantibody level might be used as an objective indication for the determination of ~(60)Coradiotherapy effect.     

肝癌单克隆抗体HAb18及其碘标记物的质控研究与应用

肝癌单克隆抗体ＨＡｂ１８及其碘标记物的质控研究与应用陈志南，刘智广，米力，仇凯，隋延仿，刘彦仿，刘成刚，徐力青，曲萍（病理学教研室８６３肝癌导向药物课题组）关键词肝肿瘤，抗体，单克隆；ＨＡｂ１８，质量控制中图号Ｒ３９２近年单克隆抗体在人类疾病的治疗和...     

肝癌单克隆抗体HAb18-1血清学诊断肝癌的应用

单克隆抗体技术的发展提供了一个有效的手段去发现一些新的肿瘤相关抗原(TA-A)或肿瘤抗原新的位点,并可检测这些抗原的表达。 本文报告应用本室制备的肝癌单抗H-Ab 18-1与郑州搏赛生物试剂实验研究所国