静脉注射异搏定终止室上性心动过速的心电图变化

我院自1994年1月至1995年12月应用静脉注射异搏定治疗阵发性室上性心动过速39例(48例次),现将该药有效转复的心电图变化报道如下: 1 资料与方法 1.1 临床资料 本组病例39例(48例次)均为男性;年龄最小45岁,最大64岁,平均49岁。病程为3个月至5年。除有冠心病4例,高血压3例外,其余均为无器质性心脏病。室上性心动过速的发作均经心电图证实。39例中共有48例次的发作,其中3次发作的2例;2次发作的5例,1次发作的32例。 1.2 方法 异搏定静脉注射剂量为0.1～0.2mg/kg/     

A Clinical Study on the Treatment of Chronic Pelvic Inflammation of Qi-stagnation with Blood Stasis Syndrome by Penyanqing Capsule (盆炎清胶囊)

Objective: To observe the clinical efficacy of Penyanqing Capsule (盆炎清胶囊, PYQC) in treating pelvic inflammation of Qi-stagnation with blood stasis syndrome. Methods: The randomized, single blinded, parallel positive drug controlled method was adopted, with 82 patients assigned into two groups by envelop method. The 42 patients in the treated group received PYQC 3 times a day, 4 capsules each time taken orally; the 40 patients in the control group were given orally Fuyankang tablets (妇炎康片, FYKT) 3 times a day, 6 tablets each time. The therapeutic course for both groups was 2 months, and 2 courses of treatment were given successively to observe the comprehensive effect, changes of symptoms and signs before and after treatment. The effects of PYQC on hemorrheological character in part of the patients and on the pathogenetic chlamydia and mycoplasma were also observed. Results: The total effective rate in the treated group was 83.3 %, which was insignificantly different from that in the control group ( 77.5 %, P ＞ 0.05 ).However, PYQC could significantly lower the hemorrheologic indexes in patients and showed definite influence on the pathogenetic chlamydia and mycoplasma. Conclusion: PYQC has good therapeutic effect in treating chronic pelvic inflammation of Qi-stagnation with blood stasis syndrome, and showed definite effect on chlamydia and mycoplasma.     

127例乙肝表面抗体ELISA检测与化学发光法的比较

Objective To compare the difference between ELISA and chemiluminescence assay in de-tecting hepatitis B surface antibody (HBsAb) serum valued in 5～100mIU/ml. Method 127 HBsAb serum samples that had valued by chemiluminescence were detected using 10 domestic ELISA kits. The correlation between the results of ELISA kits and chemiluminescence assay were analyzed. Coincidence between the results of ELISA kits and chemiluminescence at different OD value range were also analyzed. Results The detected results between 10 domestic ELISA kits and chemiluminescence assay are positive correlation (P<0.01); The optimal coincidence rates of the results of ELISA with those samples valued by chemiluminescence at more than 100mIU/ml was 99.4%; the coinci-dence rate is from 42% to 80% in cut-off±20%. The average coincidence rate is under 60%. Conclusions Though the detected results of 10 domestic ELISA reagents is related closely, the coincidence rate of the results at 5～10mIU/ ml in detecting anti-HBs is low. So it is important to make a positive standard in detecting anti-HBs.     

血清胸苷激酶1检测及其在肿瘤治疗监测和预后中的作用

目的探讨胸苷激酶1(sTK1)在肿瘤诊断和预后判断中的价值。方法利用免疫印迹增强化学发光法检测肿瘤患者组、良性疾病组和正常对照组的血清sTK1浓度及阳性率。结果sTK1诊断肿瘤的灵敏度是74·1%,特异度是97·3%;肿瘤患者组sTK1浓度为0·99~40·63pmol/L,平均浓度为4·70pmol/L,阳性率74·1%,明显高于良性疾病组(浓度为0·54~7·14pmol/L,平均浓度为1·70pmol/L,阳性率18·1%)和健康人对照组(浓度为0·14~2·15pmol/L,平均浓度为0·53pmol/L,阳性率2·73%),差异有显著性(P<0·001);肿瘤患者未经治疗组、治疗效果不佳组与治疗缓解组sTK1平均浓度比较,差异有显著性(P<0·001)。结论血清胸苷激酶1是肿瘤诊断和预后判断的有价值的指标。     

基于日常检测结果的HBsAg定性检测假阳性或假阴性的室内质量控制方法

目的建立一种利用实验室日常检测数据进行定性免疫测定假阳性或假阴性监测的室内质量控制方法。方法以实验室日常不少于20次检测的阳性率比值为基础,计算阳性率和阴性率比值的均值(-x)和标准差(s),绘制质控图,以12s为告警规则,13s和32s为失控规则。结果对实验室372批次两对半ELISA定性检测中的HBsAg实测数据进行分析,阳性率为0.20±0.05,与前20次测定的阳性率为0.21±0.04;阴性率为0.80±0.05,与前20次测定的0.779±0.049相近。372批次测定中,无论以阳性率比值还是以阴性率比值为基础,分别都能判断有18次超出x-±2s,按照所规定的质控规则有3次失控,符合统计学上的正态分布。结论临床实验室可采用日常检测数据对免疫常规检测进行假阳性或假阴性监测。     

实施ISO15189质量标准 规范医学实验室继续教育管理

继续教育质量管理体系是医院检验科整体质量管理系统中的重要组成部分。我院检验科于2005年6月通过了ISO15189实验室认可。在认可的准备期间，我们按照ISO15189要求，整理、编制了与我科人力资源管理程序配套的《检验科继续教育培训方案》和《检验科继续教育考核办法》等继续教育质量体系文件，从而确定了我科继续教育质量管理标准，规范了我科继续教育质量行为，使继续教育活动有法可依、有章可循、有程序可遵、有标准可比、有记录可查，为检验科继续教育质量管理活动奠定了坚实的基础。     

类视网膜母细胞瘤结合蛋白8基因的PCR检测

目的通过检测类视网膜母细胞瘤结合蛋白8（Similar to retinoblastoma binding protein 8,STRBP8）在人食管上皮细胞癌变过程中的差异表达,为寻找食管癌特异性标志物和研究食管癌的发病机制提供新线索。方法从GenBank中获取STRBP8的基因序列,并根据其CDS区设计相应的引物;利用TRIZOL法提取食管癌细胞总RNA,通过RT-PCR对目的基因进行扩增。结果本实验从食管癌细胞中成功扩增出STRBP8的特定目的基因。结论 STRBP8在食管癌细胞中存在表达。     

广州地区吸入过敏原引起过敏性鼻炎过敏原谱分析

目的分析广州地区过敏性鼻炎的主要吸入过敏原,以作该地区流行病学过敏原筛查及诊断分析。方法采用德国Mediwiss过敏原检测系统,检测明确诊断为过敏性鼻炎的成人患者1 170例,分析主要过敏原并排序。结果常见过敏原阳性率排序分别为户尘螨60.9%、蟑螂22.1%、点青霉烟曲霉7.9%、杨树5.0%、矮豚草4.8%、猫狗毛4.4%、蒿4.0%;男女患者过敏原阳性率比较差异无统计学意义(P>0.05);同一患者同时检出1～2种过敏原的占多数,达72.8%。结论广州地区吸入性过敏原以户尘螨、蟑螂为主,在本地区进行成人过敏性鼻炎过敏原流行病学调查时,可选择户尘螨、蟑螂、点青霉烟曲霉、杨树、矮豚草、猫狗毛等为重点。     

应用EP12-A2评价HBsAg定性分析不精密度

摘要：目的：探讨EP12-A2在乙肝病毒表面抗原（HBsAg）定性试验分析性能评价中的作用。 方法：对一个HBsAg血清S逐步稀释,用ELISA试剂重复测定40次,得到试剂A和B的批内阳性率为5%、35%~65%范围和95%的浓度样本,分别用Roche公司电化学发光仪Cobas e411测定相应的C5、C50和C95发光值。以HBsAg发光值为横坐标,ELISA试验阳性率为纵坐标绘制批内不精密度曲线。将两试剂C5_批内、C50_批内、C95_批内及C50_批内±15%各浓度点每天用ELISA试剂重复检测3次,共测10 d,计算阳性率并绘制批间不精密度曲线。比较两HBsAg试剂的C50和C5~C95区间。 结果 通过ELISA试验和电化学发光检测得出,A试剂不精密度曲线的C5、C50和C95浓度点发光值分别为9.63、10.34和10.91 COI,B试剂为3.78、4.64和5.06 COI。对两试剂C5~C95区间的分析,A试剂C50_批内±8%和B试剂C50_批内±19%包含了各自的C5~C95区间,A试剂的精密度较B试剂好。两试剂的病人样本方法学比对的结果一致。 结论:比对HBsAg试剂的C50和C5~C95区间能反映试剂的灵敏度和不精密度。不精密度曲线可应用于定性分析的性能评价,并有较好的推广价值。