HMGB1对缺氧复氧环境下心肌细胞凋亡的影响及机制研究

目的 探讨高迁移率蛋白1(HMGB1)对缺氧复氧环境下心肌细胞凋亡的影响及机制。 方法 心肌细胞H9C2分为正常组、H/R组、siRNA-NC组、HMGB1 siRNA组。其中正常组细胞为正常培养的H9C2细胞,H/R组、siRNA-NC组、HMGB1 siRNA组细胞分别进行缺氧复氧处理,HMGB1 siRNA组、siRNA-NC组细胞在缺氧处理前48 h分别转染HMGB1小干扰RNA(HMGB1 siRNA)和小干扰RNA阴性对照(siRNA control)。RT-PCR和Western blot检测细胞中HMGB1表达水平,流式细胞术检测细胞凋亡,试剂盒检测细胞中丙二醛(MDA)、超氧化物歧化酶(SOD)水平及培养液上清中乳酸脱氢酶(LDH)水平,Western blot检测信号转导与转录因子3(STAT3)、磷酸化的STAT3(p-STAT3)、活化的含半胱氨酸的天冬氨酸蛋白水解酶3(Cleaved Caspase-3)蛋白表达水平。 结果 与正常组相比,H/R组细胞中HMGB1 mRNA、HMGB1蛋白、细胞凋亡率、LDH和MDA、 Cleaved Caspase-3水平显著升高(P<0.05),而SOD、p-STAT3水平明显降低,差异均有统计学意义(P<0.05)。siRNA-NC组细胞中HMGB1 mRNA和蛋白水平、细胞凋亡率、LDH、MDA及SOD水平、STAT3、p-STAT3、Cleaved Caspase-3水平与H/R组相比差异无统计学意义(P>0.05)。与H/R组相比,HMGB1 siRNA组细胞中HMGB1 mRNA、HMGB1蛋白、细胞凋亡率、LDH和MDA、 Cleaved Caspase-3水平明显降低,而SOD、p-STAT3水平明显升高,差异均有统计学意义(P<0.05)。 结论 HMGB1表达下调可能通过降低缺氧复氧对心肌细胞的氧化损伤而抑制心肌细胞凋亡。     

沉默MyD88抑制NF-κB p65活化对TNF-α诱导的心肌细胞凋亡和炎症损伤的保护作用

为探究沉默髓样分化因子88(myeloid differentiation factor 88,MyD88)抑制NF-κB p65活化对TNF-α诱导的心肌细胞凋亡和炎症损伤的影响,采用TNF-α处理心肌细胞,将其随机分为4组:Control组、TNF-α组、TNF-α+shRNA-NC组和TNF-α+sh-MyyD8...     

脐血干细胞经冠状动脉移植可降低心律失常评分及室性心律失常发生率

BACKGROUND:Until now, it is still unclear whether the stem cell transplantation triggers adverse effects on the myocardial electrical activity, resulting in the emergence of arrhythmia. OBJECTIVE:To explore the effect of intracoronary transplantation of umbilical cord blood stem cells on arrhythmia score and incidence of ventricular arrhythmia. METHODS:According to therapeutic strategies, 73 patients with coronary heart disease were assigned to receive drug therapy in control group (n=38) and umbilical cord blood stem cell transplantation in observation group (n=35). Arrhythmia score, incidence of ventricular arrhythmia and adverse reactions were recorded and analyzed before and 1, 4, 8 weeks after transplantation. RESULTS AND CONCLUSION:After treatment, arrhythmia scores were significantly reduced in the two groups, especially in the observation group, to exhibit a continuous decline trend (P < 0.05). Compared with the control group, the incidence of ventricular arrhythmia was significantly lower in the observation (P < 0.05). However, there were no significant changes in the blood pressure, heart rate and blood oxygen saturation before and after transplantation, and no acute heart failure and death occurred in the two groups. These results suggest that the intracoronary transplantation of umbilical cord blood stem cells exhibits superiorities in the treatment of coronary artery disease, significantly reducing the arrhythmia score, reducing the incidence of ventricular arrhythmia, and resulting in less adverse reactions.     

醋柳黄酮对大鼠离体主动脉环的舒张作用及机制研究

目的:探讨醋柳黄酮(TFH)对大鼠离体胸主动脉环的舒张作用及其与内皮的关系.方法:采用离体血管环技术制备大鼠胸主动脉环,并置于灌流槽中.使用累积加药法,检测TFH对去甲肾上腺素(NE)预收缩的胸主动脉环张力的影响,并分为去内皮组及非去内皮组,对TFH扩血管作用对照研究.结果:TFH对离体大鼠内皮完整和去内皮的胸主动脉环均呈剂量(浓度为0.02g/L;0.04g/L;0.06g/L)依赖性扩血管作用,THF在0.02g/L;0.04g/L的浓度对内皮完整的主动脉环较去内皮主动脉环舒张血管作用更强,有统计学差异(P＜0.05),在0.06g/L的浓度两组均为完全舒张,最大舒张反应均为100%.结论:THF呈剂量依赖性扩血管作用,其舒张血管作用在小剂量呈内皮依赖性,而大剂量呈非内皮依赖性.     

新乡市社区老年人慢性病现状及其影响因素

我们对社区老年人高血压、糖尿病、冠心病、脑卒中四种病的现状及其影响因素进行了调查分析。社区内３９５３６人中６０岁以上的老年人４７６６人,采用“整群＋系统”二级抽样,应答人数２４５３人（男７１６,女１７３７）,应答率８７．１１％。采用统一调查表,诊断分...