小鼠异体Sertoli细胞诱导胰岛移植免疫耐受的作用

Objective To study whether the sertoli cell allograft can achieve the immunotolerance and protect the co-transplant islet allograft on the heterotopic situation. Methods The diabetic C57 mice were used as recipients, and healthy BALB/C mice as islet donors,respectively. Healthy BALB/C and C57 mice were used as testis sertoli cell donors. The recipients were randomly divided into 4 groups,6 mice in each group : group A: only transplant with islet allograft;group B: co-transplant with islet allograft and serto-li isograft;group C:co-transplant with islet allograft and sertoli allograft;group D:sham-operated group. The blood and urine glucose levels in the models, and the survival time of the graft were observed. Results The mean survive time of graft in groups A, B, and C was (6.50±2.35 ), (55.67±4.84), and (51.33± 5.05 ) days respectively. In group D, blood glucose level was abnormal. The hyperglycemia of the diabetic C57 mice could be reversed by the transplant methods of groups B and C. The mean survival time in groups B and C was longer than in group A P < 0.05, but there was no significant differences between groups B and C,P > 0.05. Conclusion The sertoli cells can induce local immunotolerance and protect the co-transplant islet allograft. Sertoli cell isograft can obtain the same local immunotolerance as the sertoli cell allograft.     

盆筋膜解剖及其与直肠固有筋膜关系研究

目的 探讨盆筋膜的结构、范围及其与直肠固有筋膜的层次关系.方法 2020年12月至2021年3月对由广东药科大学生命与生物制药学院人体解剖与胚胎学系及中山大学中山医学院人体解剖学教研室提供的12具(男性5具、女性7具)骨盆标本进行大体观察,并对Denonvilliers筋膜进行组织学观察.结合2019年12月至2021...     

小鼠异体Sertoli细胞诱导胰岛移植免疫耐受的作用

Objective To study whether the sertoli cell allograft can achieve the immunotolerance and protect the co-transplant islet allograft on the heterotopic situation. Methods The diabetic C57 mice were used as recipients, and healthy BALB/C mice as islet donors,respectively. Healthy BALB/C and C57 mice were used as testis sertoli cell donors. The recipients were randomly divided into 4 groups,6 mice in each group : group A: only transplant with islet allograft;group B: co-transplant with islet allograft and serto-li isograft;group C:co-transplant with islet allograft and sertoli allograft;group D:sham-operated group. The blood and urine glucose levels in the models, and the survival time of the graft were observed. Results The mean survive time of graft in groups A, B, and C was (6.50±2.35 ), (55.67±4.84), and (51.33± 5.05 ) days respectively. In group D, blood glucose level was abnormal. The hyperglycemia of the diabetic C57 mice could be reversed by the transplant methods of groups B and C. The mean survival time in groups B and C was longer than in group A P < 0.05, but there was no significant differences between groups B and C,P > 0.05. Conclusion The sertoli cells can induce local immunotolerance and protect the co-transplant islet allograft. Sertoli cell isograft can obtain the same local immunotolerance as the sertoli cell allograft.     

以问题为基础学习结合病案讨论教学法在普通外科学见习教学中的应用

Objective To investigate the application effect of 4 teaching methods, traditional teaching method, problem-based learning (PBL), case-based discussion and PBL combined with casebased discussion in clerkship teaching of general surgery. Methods One hundred and thirty-two clinical probationers of grade 2003 and 2004 were divided into 4 groups (33 in each group). Four teaching methods were applied respectively. Performance records of theoretical test, clinical skill assessments and questionnaires were analyzed statistically at the end of clerkship. Results The results of PBL and case-based discussion teaching were superior to traditional teaching ( P ＜ 0.05 ). And the result of PBL combined with case-based discussion teaching was superior to the others (P ＜0.05). Conclusions PBL combined with case-based discussion teaching method can stimulate clinical probationers' learning interests, promote the training of clinical thinking and elevate their learning efficiency.     

胚胎猪皮移植效果的实验研究

目的:探讨胚胎猪皮作为烧伤创面移植覆盖物的可行性,为临床应用提供一种新的创面覆盖物。方法:通过对实验动物(家兔、幼猪)进行胚胎猪皮和幼猪皮的异体、异种皮移植,观测移植效果,利用免疫组织化学方法及Western blot检测观察移植物内CD8+和CD4+T淋巴细胞的浸润情况。结果:家兔异种皮移植效果最差;幼猪异体皮移植效果次之;胚胎猪皮无论是在异体还是异种移植效果上均强于同组幼猪皮移植;CD8+和CD4+细胞计数值及其蛋白表达明显低于同组幼猪皮移植。结论:胚胎猪皮抗原性小,可作为一种新的创面覆盖物应用于临床研究。     

全反式维甲酸提高人结肠癌细胞亚株SW480/M5对奥沙利铂敏感性

目的探讨全反式维甲酸(all-trans retinoic acid,ATRA)提高人结肠癌细胞亚株SW480/M5对奥沙利铂(oxaliplatin,L-OHP)敏感性的可能机制.方法 MTT法筛选ATRA和L-OHP实验浓度.流式细胞仪检测ATRA对肿瘤细胞周期影响.分别用ATRA、L-OHP、ATRA联合L-OHP作用SW480/M5细胞,MTT法检测药物对肿瘤细胞抑制率,流式细胞仪检测肿瘤细胞周期及凋亡率,原子光谱吸收仪检测肿瘤细胞DNA含铂(Pt)量. 结果L-OHP抑制SW480/M5细胞增殖的GI50为58.0mg/L,主要阻滞肿瘤细胞在S和G2/M期.ATRA 8.0μmol/L作用24小时后,G1期细胞减少,S期细胞增多;作用72小时后,S期和G2/M期细胞增加并明显抑制肿瘤细胞增殖.8.0μmol/LATRA作用至48小时后联合L-OHP,两药联合由相加作用转变为协同作用;联合用药后S期和G2/M期细胞明显增多,细胞DNA含Pt量显著增加,呈时效依赖性.相对于单独用药,联合用药并不上调肿瘤细胞凋亡率.结论ATRA通过改变SW480/M5细胞周期和提高细胞DNA含Pt量,明显增加肿瘤细胞对L-OHP敏感性.     

应用基因芯片技术筛选结直肠癌及癌旁组织基因的差异表达

目的用基因芯片技术筛选结直肠癌及癌旁组织基因差异表达,寻找结直肠癌相关基因并了解其在肿瘤发生及转移过程中的作用。方法选用有8000条人类基因的cDNA表达谱芯片,以6例临床手术切除的结直肠癌组织、癌旁3cm和5cm肠黏膜组织及正常肠黏膜组织为研究对象,筛查相关基因的差异表达。结果与正常对照组相比．在癌组织样品中存在显著性表达差异的基因有769个,其中发生上调表达的有363个．下调表达的有406个：在癌旁3cm样品中存在显著性表达差异的基因有155个．其中发生上调表达的有52个．下调表达的有103个：在癌旁5cm样品中存在显著性表达差异的基因有230个．其中发生上调表达的有46个,下调表达的有184个。在癌组织与正常对照组间,具有差异表达的基因较多（19．5％．769／3944）,而在癌旁3Cm、5cm两组直肠黏膜组织与正常对照组间．差异表达的基因则较少．占总体基因数量的3．9％～5．8％。差异表达的基斟中具有明显功能类型的基因主要包括肿瘤相关基因、细胞增殖与凋亡调控基因、基因转录调控基因及细胞外基质成份及其降解相关的基因。结论不同来源组织间在生物学性质上具有各自的特性,多种基因参与了结直肠癌的发生与发展。癌旁组织也存在着基因的差异表达．显示其也有恶变倾向。     

小鼠异体Sertoli细胞诱导胰岛移植免疫耐受的作用

Objective To study whether the sertoli cell allograft can achieve the immunotolerance and protect the co-transplant islet allograft on the heterotopic situation. Methods The diabetic C57 mice were used as recipients, and healthy BALB/C mice as islet donors,respectively. Healthy BALB/C and C57 mice were used as testis sertoli cell donors. The recipients were randomly divided into 4 groups,6 mice in each group : group A: only transplant with islet allograft;group B: co-transplant with islet allograft and serto-li isograft;group C:co-transplant with islet allograft and sertoli allograft;group D:sham-operated group. The blood and urine glucose levels in the models, and the survival time of the graft were observed. Results The mean survive time of graft in groups A, B, and C was (6.50±2.35 ), (55.67±4.84), and (51.33± 5.05 ) days respectively. In group D, blood glucose level was abnormal. The hyperglycemia of the diabetic C57 mice could be reversed by the transplant methods of groups B and C. The mean survival time in groups B and C was longer than in group A P < 0.05, but there was no significant differences between groups B and C,P > 0.05. Conclusion The sertoli cells can induce local immunotolerance and protect the co-transplant islet allograft. Sertoli cell isograft can obtain the same local immunotolerance as the sertoli cell allograft.     

阑尾套叠的诊治

目的 探讨阑尾套叠的特征和诊治方法.方法 回顾1990年1月至2006年12月收治的5例阑尾套叠患者的临床资料,结合文献报道,就该病特点及诊治经验进行探讨.结果 本组2例表现为定位不明确、反复发作绞窄性腹痛;2例表现为急性阑尾炎症状;1例腹部可触及包块.5例患者中2例术前行腹部B超检查,腹部X片检查3例,纤维结肠镜检查1例.所有病例均行手术治疗,术中发现阑尾套叠,4例行阑尾切除术,1例行常规阑尾切除及盲肠壁修补术.术后5例患者痊愈出院.结论 阑尾套叠是一种非常少见的疾病,其临床表现多变,术前很难作出诊断;纤维结肠镜检查能够协助诊断,但是常误诊为盲肠息肉而摘除,引起肠瘘,甚至休克死亡;手术是主要的治疗方法.     

小鼠异体Sertoli细胞诱导胰岛移植免疫耐受的作用

Objective To study whether the sertoli cell allograft can achieve the immunotolerance and protect the co-transplant islet allograft on the heterotopic situation. Methods The diabetic C57 mice were used as recipients, and healthy BALB/C mice as islet donors,respectively. Healthy BALB/C and C57 mice were used as testis sertoli cell donors. The recipients were randomly divided into 4 groups,6 mice in each group : group A: only transplant with islet allograft;group B: co-transplant with islet allograft and serto-li isograft;group C:co-transplant with islet allograft and sertoli allograft;group D:sham-operated group. The blood and urine glucose levels in the models, and the survival time of the graft were observed. Results The mean survive time of graft in groups A, B, and C was (6.50±2.35 ), (55.67±4.84), and (51.33± 5.05 ) days respectively. In group D, blood glucose level was abnormal. The hyperglycemia of the diabetic C57 mice could be reversed by the transplant methods of groups B and C. The mean survival time in groups B and C was longer than in group A P < 0.05, but there was no significant differences between groups B and C,P > 0.05. Conclusion The sertoli cells can induce local immunotolerance and protect the co-transplant islet allograft. Sertoli cell isograft can obtain the same local immunotolerance as the sertoli cell allograft.