老年Colles骨折患者门诊治疗观察

目的观察老年Colles骨折门诊行手法复位石膏夹板外固定的临床疗效。方法回顾性分析2009年1月－2010年1月本院门诊行手法复位石膏夹板外同定治疗的老年Colles骨折患者50例临床资料。结果本组50例患者骨折均愈合良好,外固定4周,获得随访49例,失访1例,随访时间6—14个月,平均10．5个月,并发症：功能受限2例,萎缩性骨炎1例。结论老年coUes骨折门诊手法复位外固定治疗简便、安全、有效、费用低廉,值得基层医院推广。     

职业卫生现场采样的质量控制

在职业卫生技术服务过程中,现场采样的质量控制至关重要.依据GBZ159-2004.<工作场所空气中有害物质监测的采样规范>[1]要求,职业卫生现场采样应根据工作场所具体条件和职业病危害因素的具体情况,对职业病危害因素检测项目确定、采样条件、采样方法、采样点、采样设备选择应用、样品保存运输等,严格按照国家有关的规范、标准执行.     

两种膨润土细胞毒性的体外试验研究

Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite.Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay,neutral red uptake(NRU) assay,lactate dehydrogenase(LDH) leakage assay,apoptosis assay and hemolysis assay.In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0,0.3125,0.6250,1.2500 and 2.5000 mg/ml for ten min.In other four assays,human B lymphoblast cells (HMy2.CIR) served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10,20,30,60,120 and 180 μg/ml for four h.Results In hemolysis assay,the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control (P＜0.05);in CCK-8 assay,the cellular activities in acid bentonite group at the doses ≥30 μg/ml and in organic bentonite group at the doses ≥20μg/ml were significantly lower than that of control (P＜0.01);the similar results appeared in NRU assay and LDH assay,and the dose-effect relationship was observed in above 4 assays.In apeptosis assay,the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control (P＜0.05).Moreover,the results of five in vitro assays indicated the eytotoxicity induced by organic bentonite was higher than that induced by acid bentonite.Conclusion Two kinds of bentonite could induce cytotoxicity,such as apoptosis and damage of cell membrane.The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.     

大姚县青石棉污染区主要恶性肿瘤的健康生命损失年分析

目的评价云南省大姚县环境青石棉污染对人群健康及社会造成的危害。方法通过查阅当地县级医院门诊和住院登记恶性肿瘤病例、人口死因登记报告中1994至2003年期间死亡的恶性肿瘤患者。用健康生命损失年评价当地恶性肿瘤引起的死亡、健康寿命损失及社会负担。结果1994至2003年，青石棉污染区肺癌、肝癌、间皮瘤、胃癌、结肠直肠癌的死亡率分别为10．15／10万、9．04／10万、8．48／10万、3．96／10万、3．55／10万。除女性乳腺癌外，男女各主要恶性肿瘤死亡率均随年龄增长而增长。肝癌、肺癌、问皮瘤、白血病、胃癌的健康生命损失年损失分别是1981．39、1886．63、1799．23、948．01、754．18人年。健康生命损失年损失的年龄分布主要集中于15～44岁和45～59岁年龄组。恶性肿瘤引起的间接经济损失以肺癌、肝癌、间皮瘤造成的损失较大，分别占总损失的15．02％、13．98％、13．01％。青石棉污染引起的健康生命损失年为3092．23人年，间接经济损失517．58万元。结论肺癌、肝癌、间皮瘤、白血病和女性乳腺癌对污染区人群健康和社会危害影响较大。恶性肿瘤造成的中老年人死亡较为严重，引起的青壮年死亡造成的社会损失最大。     

两种膨润土细胞毒性的体外试验研究

Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite.Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay,neutral red uptake(NRU) assay,lactate dehydrogenase(LDH) leakage assay,apoptosis assay and hemolysis assay.In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0,0.3125,0.6250,1.2500 and 2.5000 mg/ml for ten min.In other four assays,human B lymphoblast cells (HMy2.CIR) served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10,20,30,60,120 and 180 μg/ml for four h.Results In hemolysis assay,the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control (P＜0.05);in CCK-8 assay,the cellular activities in acid bentonite group at the doses ≥30 μg/ml and in organic bentonite group at the doses ≥20μg/ml were significantly lower than that of control (P＜0.01);the similar results appeared in NRU assay and LDH assay,and the dose-effect relationship was observed in above 4 assays.In apeptosis assay,the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control (P＜0.05).Moreover,the results of five in vitro assays indicated the eytotoxicity induced by organic bentonite was higher than that induced by acid bentonite.Conclusion Two kinds of bentonite could induce cytotoxicity,such as apoptosis and damage of cell membrane.The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.     

阻塞性睡眠呼吸暂停低通气综合征与儿童生长发育

阻塞性睡眠呼吸暂停低通气综合征(obstructive sleep apnea hypopnea syndrome,OS-AHS)是儿童常见疾病之一,直接关系到儿童体格、认知发育及心理健康,已逐渐受到重视。本文就近年来关于此病对儿童生长发育影响的研究做一综述。     

阻塞性睡眠呼吸暂停低通气综合征与儿童生长发育

阻塞性睡眠呼吸暂停低通气综合征（obstructive sleep apnea hypopnea syndrome,OS-AHS）是儿童常见疾病之一,直接关系到儿童体格、认知发育及心理健康,已逐渐受到重视。本文就近年来关于此病对儿童生长发育影响的研究做一综述。     

吡哌酸片溶出度光纤传感实时分析研究

 目的建立吡哌酸片剂溶出度的光纤传感实时检测方法。方法采用转篮法,转速100r·min^-1,以稀盐酸(24→1000)为溶出介质,用光纤化学传感药物溶出度实时分析系统在320nm处检测紫外吸收。同时用药典规定方法检测溶出浓度,以作相互对照。结果在50.～249μg·mL^-1内,吸光度与吡哌酸片的浓度成线性关系,平均回收率为100.4%。光纤传感实时检测方法所得结果与药典方法结果无显著性差异(P>0.05)。结论所建立的吡哌酸片剂溶出度的光纤传感实时检测方法可行,在位在线、连续定量地反映了药物体外溶出特性。     

两种膨润土细胞毒性的体外试验研究

Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite.Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay,neutral red uptake(NRU) assay,lactate dehydrogenase(LDH) leakage assay,apoptosis assay and hemolysis assay.In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0,0.3125,0.6250,1.2500 and 2.5000 mg/ml for ten min.In other four assays,human B lymphoblast cells (HMy2.CIR) served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10,20,30,60,120 and 180 μg/ml for four h.Results In hemolysis assay,the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control (P＜0.05);in CCK-8 assay,the cellular activities in acid bentonite group at the doses ≥30 μg/ml and in organic bentonite group at the doses ≥20μg/ml were significantly lower than that of control (P＜0.01);the similar results appeared in NRU assay and LDH assay,and the dose-effect relationship was observed in above 4 assays.In apeptosis assay,the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control (P＜0.05).Moreover,the results of five in vitro assays indicated the eytotoxicity induced by organic bentonite was higher than that induced by acid bentonite.Conclusion Two kinds of bentonite could induce cytotoxicity,such as apoptosis and damage of cell membrane.The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.     

自制复方甲硝唑痤疮擦剂治疗痤疮50例

痤疮是青少年人的多发病、常见病。人群中约有80％-90％的人患过此病。它不仅影响人的容貌美，而且带来精神和肉体上的痛苦。目前，治疗方法各异，疗效不甚显著。我院自2001-2005年，采用自制复方甲硝唑擦剂治疗痤疮50例，取得满意的效果，现报告如下。