云南重楼与球药隔重楼皂苷含量差异及光合特性研究

目的 了解云南重楼与球药隔重楼皂苷含量差异及光合特性,为重楼人工栽培及药用资源开发利用奠定理论基础。方法 以6年生云南重楼和球药隔重楼为材料,使用HPLC测定皂苷含量,LI-6800光合测定仪测定光合参数,紫外吸收法测定生化参数,石蜡包埋法测定叶片显微结构。结果 云南重楼与球药隔重楼皂苷含量及光合特性具有较大差异：(1)球药隔重楼总皂苷含量较云南重楼高118.97%,云南重楼中薯蓣皂苷类含量占总皂苷的95.86%;(2)球药隔重楼光补偿点仅为7.20μmol·m^-2·s^-1,且净光合速率、蒸腾速率、气孔导度、胞间CO₂浓度/环境CO₂浓度均显著低于云南重楼;(3)球药隔重楼叶绿素b含量显著高于云南重楼,且叶绿素a/b含量显著低于云南重楼;(4)云南重楼可溶性糖、可溶性蛋白及脯氨酸含量显著高于球药隔重楼,MDA含量显著低于球药隔重楼;(5)球药隔重楼叶片厚度、栅海比显著高于云南重楼。结论 球药隔重楼具有较高的皂苷含量且具有更好的抗寒性及耐阴性,可以考虑将球药隔重楼作为云南重楼的替代品进行深入研究与开发...     

《鼻炎鼻窦炎个体化治疗与调养》出版: 儿童慢性鼻窦炎的内科治疗

《鼻炎鼻窦炎个体化治疗与调养》一书是由周作新主编,由河南科学技术出版社于2017年出版.该书介绍了鼻炎鼻窦炎的定义、临床症状、病理、病因、基础检查、临床诊断等基础知识,重点阐述鼻炎鼻窦炎药物治疗及预防措施,涉及西药治疗、中药方剂治疗、中成药治疗、营养调养及新型治疗方法.全书结构层层递进,叙述深入浅出,结合生活案例解读鼻...     

浅谈现代护理工作者应具备的能力

随着现代医学科学的发展,护理学科在护理理论、护理技术、护理体制和护理范围上都得到了不断的更新和发展。21世纪护理学的知识系统已由单一的患者扩大到全社会的人群,护士也已由从属地位变成独立的科技工作者。这无疑对     

提上睑肌腱膜修复治疗老年性后发性上睑下垂

目的 探讨与运用手术疗法,治疗因内眼手术导致的老年性后发性上睑下垂.方法 采用提上睑肌腱膜缝合术与腱膜折叠术进行修复矫正.结果 32例60眼进行腱膜缝合术,16例32眼进行腱膜折叠术,成功率为90%.结论 本组手术疗法不难看出,提上睑肌膜的修复,其上睑缘位置得到满意的提高,其功能得到强化与巩固,其活动度也相应地增强,得到了与传统的上睑下垂矫正不能达到的效果.     

神经外科意识障碍患者的转运护理

目的 讨论意识障碍患者的安全转运护理.方法 对意识障碍患者的安全护理总结. 结果意识障碍患者院内转运安全. 结论转运前认真评估患者病情,妥善处理病人所需,在转运前加强与接收部门的沟通,准备所需的仪器及药物,指派合格人员随行,以保证意识障碍患者院内转运安全,减少不良事件发生.     

内分泌和化疗对乳腺肿瘤患者干细胞的影响

BACKGROUND:Tumor stem cells are the root of cancer recurrence and metastasis, so clinical researches should focus on the effects of different treatments on tumor stem cells. OBJECTIVE:To explore the effects of endocrine therapy and chemotherapy on stem cells in patients with breast cancer. METHODS:After recovery and cultivation of estrogen receptor-positive human breast cancer cell lines MCF-7, passage 3 cells in logarithmic phase were selected and divided into three groups containing control, estradiol and estradiol with tamoxifen groups. The estradiol group was divided into three subgroups: 10^-7, 10^-8 and 10^-9 mol/L estradiol was added into the medium, respectively; the estradiol with tamoxifen group was divided into three subgroups: 10^-7, 10^-8 and 10^-9 mol/L estradiol with 10^-6 mol/L tamoxifen were added into the medium, respectively. The same amount of absolute ethyl ethanol was added into the medium of control group. Fifteen female patients with late recurrence and metastasis of breast cancer received chemotherapy as recurrence and metastasis group. Another 15 healthy volunteers were selected as healthy control group. RESULTS AND CONCLUSION:The proportion of CD44⁺CD24^-/low cell subsets in the estradiol and estradiol with tamoxifen groups was significantly higher than that of the control group (P < 0.05), and the proportion of CD44⁺CD24^-/low cell subsets in the estradiol group was significantly higher than that of the estradiol with tamoxifen group at the same concentration (P < 0.05). The proportion of CD44+CD24-/low cell subsets had no significant differences among groups at 10 and 20 days of culture (P < 0.05). The proportion of CD44⁺CD24^-/low cell subsets significantly increased in MCF-7 cells after 24-hour intervention with different chemotherapy drugs. But only the proportion of CD44⁺CD24^-/low cell subsets in the paclitaxel and doxorubicin groups was significantly higher than that of the control group after 20-day intervention (P < 0.05). Besides, the proportion of CD44⁺CD24^-/low cell subsets in the peripheral blood of healthy volunteers was significantly lower than that of the recurrence and metastasis group (P < 0.05). Among 15 patients with late recurrence and metastatic of breast cancer, 9 had stable disease, 5 had partial remission, 1 had failed chemotherapy and cancer progression. Moreover, the proportion of CD45^-CD44⁺CD24^-/low cell subsets in the peripheral blood of patients sensitive for chemotherapy was significantly lower than that before treatment (P < 0.05). In conclusion, both endocrine therapy and chemotherapy exert a certain effect on the CD44⁺CD24^-/low cell subsets of breast cancer positive for estrogen receptor. Given that CD44⁺CD24^-/low cell subsets in MCF-7 cells resist chemotherapy drugs, the proportion of CD45^-CD44⁺CD24^-/low cells in the peripheral blood of patients sensitive for chemotherapy is decreased.     

男性全身麻醉病人留置尿管时机对麻醉恢复期的影响

留置尿管是基础护理中最常见的技术操作之一,指在严格无菌操作下,用导尿管经尿道插入膀胱引流尿液的方法,是手术病人一项重要的术前准备,是诊断、治疗各种急、危重病人及手术病人必要的护理措施,也是预防手术病人尿潴留、观察尿量、了解病情的重要措施[1]。以往是在病房为手术病人完成这一操     

单切口腹腔镜前列腺癌根治围手术期护理体会

关键词： 前列腺癌 单切口腹腔镜 根治术 护理 前列腺癌在欧美是最常见的恶性肿瘤之一,在我国比较少见,而近年来发病率有逐渐上升趋势。腹腔镜前列腺癌根治术是近年发展起来的微创外科新技术,国外开展较多 ,国内报道极少,2010年6月–2010年8月我院开展了5例单切口腹腔镜前列腺癌根治切除术,取得了较好的早期疗效,手术顺利其优点如：单切口（辅助小切口）手术可以降低手术切口出血、感染、切口疝的发生率。     

护士对礼仪知识需求的现状及调查分析

目的调查护理人员对文明礼仪知识需求的现状及掌握程度,为针对性培训提供参考依据。方法采用自行设计的问卷,通过抽签法选取150名临床护士进行调查,内容包括临床护士自身整体礼仪知识水平的评价、对礼仪知识的认知、护士学习礼仪知识的意愿、护士对礼仪知识需求的排序。结果临床护士整体礼仪知识水平仍需提高;护士对礼仪知识的学习有强烈的意愿,但缺乏规范化和系统化。结论在职护士礼仪知识掌握的程度与医疗市场的需求仍存在差距,医院需要将此项培训内容列入培训计划,采取灵活多样的培训方法,以提高护士的文明素质,规范礼貌服务的护理行为。     

赤峰地区平衡易位致习惯流产2例报告

染色体平衡易位携带者在人群中占0.47%,本文检出2例,1男1女,表型正常,上染色体平衡易位而致习惯流产。