前癃通胶囊介导miR-216a-5p/TPT1/mTORC1通路调控良性前列腺增生的实验研究

目的 通过细胞实验探讨前癃通胶囊（qian long tong capsule, QLTC）能否通过调控miR-216a-5p/肿瘤蛋白翻译控制1/哺乳动物雷帕霉素靶蛋白复合物1(miR-216a-5p/tumor protein translationally controlled 1/mammalian target of rapamycin complex 1,miR-216a-5p/TPT1/mTORC1)信号通路抑制良性前列腺增生（benign prostatic hyperplasia, BPH）。方法 将25只大鼠随机分为对照组（等体积生理盐水）,QLTC低（56.25 mg/mL）、中（112.50 mg/mL）、高（225.00 mg/mL）剂量组,LBSC组（168.75 mg/mL）,每组5只。每组灌胃1 mL/次,2次/d,连续5 d。各组大鼠麻醉后制备含药血清。根据实验目的不同,将CP-H022细胞分5步做实验处理,每部分实验进行独立分组。将miR-216a-5p过表达和沉默表达,及TPT1过表达进行对照研究;RT-qPCR法检测正常和BPH模型CPH022细...     

慢性非细菌性前列腺炎/慢性盆腔疼痛综合征患者前列腺液中炎症因子差异表达的临床意义

Objective To investigate the role of inflammatory cytokines in the pathogenesis of chronic non-bacterial prostatitis/chronic pelvic pain syndrome (CAP/CPPS) patients. Methods The 38 cases with CAP/CPPS patients (18 cases of CAP and 20 cases of CPPS) and 20 cases of healthy controls were selected. The differential expressions of 40 kinds of inflammatory cytokines were detec-ted by antibody arrays in prostate fluid. Results The inflammatory cytokines which increased more than 1.5 times expression have been found. There were seven kinds in CAP including monocyte che-moattractant protein (MCP)-1, solution tumor necrosis factor receptor Ⅱ(s TNF R Ⅱ), platelet-de-rived growth faetor-BB (PDGF-BB), interleukin (IL)-β, IL-11、IL-6、MCP-2 and five kinds in CPPS groups including MCP-1、PDGF-BB、MCP-2、s TNF R Ⅱ、It-11 respectively, compared with healthy control group. The cluster analysis results showed that protein expression of Monocyte chemoattrac-tant protein 1 (MCP-1)and platelet-derived growth factor BB (PDGF-BB) were significantly increased in CAP (3.47 and 2.07 times) and CPPS (2.25 and 2.19 times) compared with healthy control group and were the final polymerization of inflammatory cytokines. The protein expression of interleukin 1 β (IL-1 β), MCP-1 and soluble tumor necrosis factor Ⅱ (s TNF R Ⅱ) in CAP group was increased more than 1.85,1.55,1.67 times compared with CPPS group. Conclusions Elevated expression of inflammatory cytokines may play an important role in the course of CAP/CPPS disease. The extent of the inflammatory response of CAP was higher than CPPS. The inflammatory factors of MCP-1 and PDGF-BB could serve as a novel diagnostic marker.     

慢性非细菌性前列腺炎/慢性盆腔疼痛综合征患者前列腺液中炎症因子差异表达的临床意义

Objective To investigate the role of inflammatory cytokines in the pathogenesis of chronic non-bacterial prostatitis/chronic pelvic pain syndrome (CAP/CPPS) patients. Methods The 38 cases with CAP/CPPS patients (18 cases of CAP and 20 cases of CPPS) and 20 cases of healthy controls were selected. The differential expressions of 40 kinds of inflammatory cytokines were detec-ted by antibody arrays in prostate fluid. Results The inflammatory cytokines which increased more than 1.5 times expression have been found. There were seven kinds in CAP including monocyte che-moattractant protein (MCP)-1, solution tumor necrosis factor receptor Ⅱ(s TNF R Ⅱ), platelet-de-rived growth faetor-BB (PDGF-BB), interleukin (IL)-β, IL-11、IL-6、MCP-2 and five kinds in CPPS groups including MCP-1、PDGF-BB、MCP-2、s TNF R Ⅱ、It-11 respectively, compared with healthy control group. The cluster analysis results showed that protein expression of Monocyte chemoattrac-tant protein 1 (MCP-1)and platelet-derived growth factor BB (PDGF-BB) were significantly increased in CAP (3.47 and 2.07 times) and CPPS (2.25 and 2.19 times) compared with healthy control group and were the final polymerization of inflammatory cytokines. The protein expression of interleukin 1 β (IL-1 β), MCP-1 and soluble tumor necrosis factor Ⅱ (s TNF R Ⅱ) in CAP group was increased more than 1.85,1.55,1.67 times compared with CPPS group. Conclusions Elevated expression of inflammatory cytokines may play an important role in the course of CAP/CPPS disease. The extent of the inflammatory response of CAP was higher than CPPS. The inflammatory factors of MCP-1 and PDGF-BB could serve as a novel diagnostic marker.     

复方苍耳虫喷雾剂治疗术后切口感染36例

目的:观察复方苍耳虫喷雾剂治疗术后切口感染的临床疗效。方法:将72例切口感染患者随机分成治疗组和对照组,分别以复方苍耳虫喷雾剂和庆大霉素换药,观察创面水肿消退时间、渗液干净时间、创面愈合时间以及不同时间相创面愈合数。结果:在促进创面水肿消退、渗液干净方面,治疗组和对照组无显著差异(P>0.05);在促进创面愈合方面,治疗组和对照组有显著差异(P<0.05)。结论:复方苍耳虫喷雾剂具有清热解毒、活血化瘀、祛腐生肌之功效,能明显缩短创面愈合时间,具有杀菌抑菌、促进肉芽生长、加速创面愈合的作用。     

前癃通胶囊对良性前列腺增生模型大鼠前列腺组织病理形态学的影响

目的探讨前癃通胶囊对良性前列腺增生(BPH)大鼠前列腺组织病理形态学的影响。方法采用双侧睾丸与附睾切除术联合经背皮下注射丙酸睾酮复制BPH大鼠模型,将造模成功60只BPH大鼠随机分为前癃通胶囊高、中、低剂量组和对照组及模型组,每组12只,另外选取12只大鼠作为空白组。从术后第8天开始至第21天,前癃通胶囊低、中、高剂量组分别给予前癃通胶囊溶液0.51、1.02、2.04 g/(kg·d)灌胃,对照组给予前列癃闭通胶囊溶液2.04 g/(kg·d)灌胃,空白组和模型组给予生理盐水10 ml/(kg·d)灌胃。检测大鼠体重和前列腺湿重、体积,并计算前列腺指数;观察前列腺组织的病理变化,计算前列腺腺上皮面积、间质面积。结果与空白组比较,模型组大鼠前列腺湿重、体积及指数均升高,前列腺病理显示腺体上皮细胞增生,腺上皮明显增厚,乳头样增生腺体向腺腔内凸出,腺腔间隙增宽,可见较多分泌物,前列腺腺上皮面积、间质面积均增大(P0.05或P0.01);前癃通胶囊高、中剂量组与对照组能有效改善BPH大鼠前列腺病理变化,降低前列腺湿重、体积及指数,减小前列腺腺上皮面积、间质面积(P0.05或P0.01);且前癃通胶囊高剂量组效果最佳,优于对照组(P0.05)。结论前癃通胶囊能改善BPH大鼠前列腺腺上皮与间质病理变化,从而缩小前列腺体积、降低前列腺指数。     

加减逍遥散联合他达拉非治疗肝气郁结型阳痿病的临床研究

目的观察加减逍遥散联合他达拉非治疗肝气郁结型阳痿病的临床疗效。方法将2018年9月至2019年3月于湖南中医药大学第一附属医院男性病科门诊就诊的肝气郁结证阳痿患者130例,按随机分配原则分为对照组65例(口服他达拉非,5mg/晚)和治疗组65例(他达拉非+加减逍遥散),两组患者均治疗12周,疗程结束后,观察并对比两组患者总有效率、勃起功能障碍国际指数(IIEF-5)评分、中医症候评分以及不良反应发生率。结果后治疗组有效率明显高于对照组(P0.05);两组IIEF-5评分较治疗前均有所提高(P0.05),且治疗组IIEF-5评分高于对照组(P0.05);与治疗前比较,对照组中医证候评分无明显下降(P0.05),而治疗组显著降低,且不良反应发生率较低。结论加减逍遥散联合他达拉非治疗肝气郁结型阳痿病有效率高于单纯他达拉非,且不良反应较少,值得推广运用。     

前列散瘀胶囊对实验性大鼠前列腺增生组织中碱性成纤维细胞表达的干预

目的:探讨前列散瘀胶囊对实验性大鼠前列腺增生组织中碱性成纤维细胞(bFGF)表达的影响。方法:建立良性前列腺增生大鼠模型,设药物高、中、低剂量组及空白组,取相同部位前列腺腹叶,以免疫组化定量技术对前列腺组织bFGF进行测试。结果:前列散瘀胶囊高剂量组的bFGF表达明显低于空白组(P<0.01)。结论:中药前列散瘀胶囊治疗前列腺增生的机理之一是该药明显抑制了前列腺组织中的bFGF的表达,进而抑制了前列腺细胞的生长。     

黄芪煎剂对肾病综合征大鼠免疫因子IL-2的影响

目的：观察黄芪煎剂对肾病综合征大鼠免疫因子IL-2（白细胞介素2）的影响,验证其对肾病综合征大鼠的治疗作用,为临床用药提供依据。方法：按Bertain等方法,制作大鼠肾病综合征模型。将雄性Wistar大鼠30只随机分为正常对照组、模型组[按6.5mg/kg体重一次性尾静脉注射阿霉素,注射后14d模型形成,并于第15日开始灌胃给予生理盐水,剂量按8ml/（kg·d）]、黄芪煎剂组[造模同模型组,并于第15日开始灌胃给予黄芪煎剂,剂量按8ml/（kg·d）],均给予灌胃14周。于给药14周后处死大鼠,采用ELISA法测定IL-2的诱生水平。结果：模型组与正常对照组的IL-2诱生水平比较有显著性差异（P〈0.05）,黄芪煎剂组与模型组的IL-2诱生水平比较具有显著性差异（P〈0.05）。结论：黄芪煎剂可以通过上调IL-2的诱生水平而提高肾病综合征大鼠的免疫功能。     

前癃通胶囊含药血浆对体外良性前列腺增生前列腺基质细胞Caspase-3表达的影响

目的 观察前癃通胶囊含药血浆对体外良性前列腺增生（BPH）前列腺基质细胞Caspase-3表达的影响.方法 对BPH患者的前列腺基质细胞进行细胞培养,分别以他莫昔芬和前癃通胶囊含药血浆为干预措施,使用免疫组织化学法检测体外培养的前列腺基质细胞中Caspase-3表达水平.结果 不同剂量的前癃通胶囊均能显著提高Caspase-3的表达水平,且其作用的量效关系表现为正相关性.结论 前癃通胶囊能够增强Caspase-3在体外培养BPH前列腺基质细胞中的表达,这可能是前癃通胶囊治疗BPH的作用机理之一.     

三橘荔核汤治疗慢性附睾炎45例临床观察

目的:观察三橘荔核汤治疗慢性附睾炎的临床疗效.方法:将90例本病患者随机分为治疗组与对照组各45例,治疗组采用中药三橘荔核汤口服,对照组采用左氧氟沙星静滴,观察比较两组治疗前后VAS疼痛评分及综合疗效.结果:两组治疗后VAS疼痛评分比较,差异有统计学意义(P<0.05),总有效率治疗组为91.1%,对照组为77.8%,...