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严重烧伤大鼠肾脏细胞凋亡及其机制的研究 总被引:2,自引:1,他引:1
目的探讨严重烧伤大鼠肾脏细胞凋亡的分子机制。方法将50只雄性Wistar大鼠随机分为烧伤组和对照组,每组25只。烧伤组造成30%TBSAⅢ度烫伤(以下称烧伤),伤后创面涂碘伏抗感染,并于伤后6 h抽取大鼠静脉血后处死,留取肾脏标本。对照组除不烫伤外,其余处理同烧伤组。采用原位缺口末端标记法检测两组大鼠肾脏细胞凋亡率。流式细胞术检测肾脏细胞中肿瘤坏死因子相关的凋亡诱导配体(TRAIL)各受体的mRNA及蛋白表达水平。同时检测大鼠血浆尿素氮(BUN)、肌酐(Cr)含量。结果烧伤组大鼠肾脏细胞的凋亡率为(32.4±1.1)%,明显高于对照组[(1.0±0.6)%,P<0.05];而大鼠肾脏组织中TRAIL诱骗受体DcR1的mRNA及蛋白表达水平显著低于正常对照组(P<0.05)。伤后6 h,烧伤组大鼠BUN值[(13.3±2.0)nmol/L]及Cr值 [(76.4±2.0)μmol/L]均明显高于对照组[(5.2±0.7)mmol/L、(40.2±2.8)μmol/L,P<0.05]。结论 TRAIL凋亡通路可能参与介导了严重烧伤大鼠肾脏细胞的凋亡。 相似文献
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Objective To study the microcirculation and structural changes, surviving area of expanded prefabricated flaps. Methods A total of 40 New Zealand rabbits were divided randomly into expanded prefabricated, expender lined, simple prefabricated and free flap groups, each consisting of 10 rabbits. For the expanded prefabricated, expender lined and simple prefabricated groups, after the femoral artery and vein were transplanted into subcutaneous tissues of abdomen, and expanders were implanted into the deeper dartos. The free flap group was a blank control group. For the expanded prefabricated group, the expansion was carried out on 7th day postoperatively. On postoperative day 52, when the expander was fully expanded, island flaps with the prefabricated vessels as the pedicles were formed. The flaps were measured by laser Doppler flowmetry, light microscopy and digital re-cording of survival arca. Results When compared with the other groups, the perfusion volume of mi-crocirculation enhanced, flaps survival improved (97.54±2.73) %, blood capillary were stronger, to-gether with microscopic changes were significant in the expanded prefabricated groups (P<0.05). Conclusion Expandedprefabricated flaps can increase the survival size of the flaps and the safety of flap transplantation. 相似文献
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软骨易于雕刻成形 ,移植后易成活 ,是整形外科常用的填充、塑形材料。软骨组织由软骨细胞、基质及埋于基质中的纤维成分所组成 ,根据基质中所含纤维成分的不同可将软骨分成透明软骨 (肋软骨、关节软骨、气管及支气管软骨 )、弹性软骨 (耳廓、外耳道及会厌软骨 )及纤维软骨 (半月板及关节盘 )。软骨移植后亦存在排异、吸收、变形等缺憾 ,近年来一直是整形外科医生的研究热点之一 ,经历了自体软骨、异体软骨、异种软骨移植以及软骨组织工程等发展阶段 ,现综述如下。1 自体移植自体软骨移植取材方便 ,移植后组织易成活且吸收较少 ,已应用于临… 相似文献
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目的 了解肿瘤坏死因子(TNF)相关的凋亡诱导配体(TRAIL)及其受体在严重烧伤大鼠胸腺组织细胞异常凋亡中的作用。方法 将50只Wistar大鼠随机分为假伤组(模拟烧伤)lO只和烧伤组40只(设伤后4、12、24、48h 4个时相点)。应用膜联蛋白A5-异硫氰酸荧光素/碘化丙啶双染法,观察大鼠胸腺组织中细胞凋亡的情况;反转录-聚合酶链反应和蛋白质印迹法检测TRAIL的死亡受体5(DR5)、DR4、诱骗受体1(DcR-1)、DcR2在大鼠胸腺组织中的表达。结果 与假伤组大鼠细胞凋亡率[(6.7±0.8)%]比较,烧伤组于伤后4h[(17.1±0.4)%]起增高,12h时[(25.2±1.1)%]达高峰,48h时仍明显高于假伤组(P〈0.05)。烧伤组大鼠胸腺组织中DR5的表达显著高于假伤组,DcR2的表达则显著低于假伤组;其余受体的表达组间相似。结论 严重烧伤后早期大鼠胸腺组织的细胞凋亡明显增加,且胸腺组织中DR5和DcR2的表达异常,提示TRAIL凋亡途径可能参与了病理性细胞凋亡过程。 相似文献