结肠康灌肠治疗溃疡性结肠炎的实验研究

结肠康灌肠治疗溃疡性结肠炎的实验研究朱纯周，蔡昌学关键词结肠康，灌肠，溃疡性结肠炎，实验研究慢性溃疡性结肠炎是慢性非感染性肠道疾病，西医认为病因尚不十分清楚，可能与免疫、菌群失调、饮食不节、遗传等因素有关。我院用中药制剂结肠康灌肠液，治疗本病取得了较...     

Livin和Survivin基因联合靶向siRNA重组表达载体的构建和鉴定

目的 构建Livin和Survivin联合靶向的siRNA重组表达载体,探讨用RNA干扰方法同时下调Livin和Survivin基因表达的增效作用.方法 通过前期的实验,分别从靶向Livin基因和Survivin基因的siRNA序列中各挑选出1对最有效的siRNA序列,然后采用1个载体编码2个shRNA技术,将其构建到同一个真核表达载体上,转化DH5a菌株,提取质粒进行酶切鉴定和测序分析.结果 经酶切鉴定和测序分析证实Livin和Survivin联合靶向的siRNA重组表达载体构建成功.结论 Livin和Survivin联合靶向的siRNA重组表达载体构建成功.     

Survivin靶向SiRNA重组表达载体的构建和鉴定

目的 构建Survivin靶向SiRNA重组表达载体.方法 根据GeneBank数据库提供的Survivin基因所有变异体的共同核苷酸序列,选择设计3对带发夹状结构的核苷酸序列,克隆到空载体pGensil-1中,转化DH5α菌株,提取质粒进行酶切鉴定和测序分析.结果 经酶切鉴定证实Survivin靶向SiRNA重组表达载体测序结果与设计完全一致.结论 Survivin靶向SiRNA重组表达载体构建成功.     

反向间接血凝试验对120株金黄色葡萄球菌产肠毒素分型的实验研究

近些年来,对金黄色葡萄球菌(简称金葡菌)肠毒素所致疾病越来越引起了国内外学者的重视。但有关葡萄球菌肠毒素引起的食物中毒,其毒素的快速检测和分型国内报道甚少。本文采用反向间接血凝试验对来自武汉市的120 株血浆凝固酶阳性金葡菌产肠毒素的情况进行了初步探讨。     

香连软胶囊的抗菌作用研究

目的:研究香连软胶囊的体外抑菌和体内抗菌效果。方法测定香连软胶囊的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。又以福氏志贺菌感染小鼠,然后用香连软胶囊灌胃,以判断其体内抗菌效果,并计算半数有效量(ED50)。结果香连软胶囊对10种常见致病菌有较强的体外抑菌作用。口服一定剂量香连软胶囊的被感染小鼠生存率明显高于阴性对照,其对小鼠的ED50为0.47g/kg·d,95%置信区间为0.24~0.94g/kg·d。结论香连软胶囊的体内、外抗菌作用均较明显,此实验结果为其临床应用提供了药效学依据。     

Induction of apoptosis of human colon cancer cells by siRNA recombinant expression vector targeting survivin gene

This study constructed siRNA recombinant expression vector targeting survivin gene and observe the apoptosis induction effect of it in human colon cancer cells, siRNA recombinant expression vector targeting survivin gene was constructed and transfected into human colon cancer cells. The effect of siRNA recombinant expression vector was detected by RT-PCR, Western blot, MTT reduction assay and flow cytometry. It was confirmed by restriction endonuclease and sequence analysis that siRNA recombinant expression vector targeting survivin gene was constructed successfully. Inhibition rate of survivin siRNA at mRNA and protein levels was 36.33% and 44.65% respectively. Growth of cancer cells was inhibited and the apoptosis rate was （17.24±2.13）%. The siRNA recombinant expression vector targeting survivin gene has been constructed successfully. It not only can inhibit the expression of survivin gene, but also can induce apoptosis in human colon cancer cells remarkably.