水蛭注射液对大白鼠血小板粘附和血小板聚集功能的影响

目的　探讨水蛭注射液对大白鼠血小板粘附性和聚集性的影响。方法　采用大白鼠 2 4只 ,随机分成两组 ,实验组给药 ,对照组以实验组同样的方法和剂量给予生理盐水 ,5d后由颈总动脉取血 ,做血小板粘附性和聚集性试验。结果　水蛭注射液对大鼠血小板粘附性和聚集性具有显著的抑制作用 ,实验组与对照组抑制率有显著的统计学意义 (P <0 0 0 1)。结论　水蛭注射液能够抑制大白鼠血小板的粘附和聚集。     

重组2型腺相关病毒-转化生长因子β3对实验性肝纤维化大鼠肝脏组织病理学和Ⅰ型胶原表达的影响

Objective To investgate the effects of TGF β 3 on rat hepatic fibrosis. Methods The TGF β 3 cDNA was cloned into rAAV2 vector. Rats were randomly divided into four groups: normal control group, model group, negative control group and TGF β 3 group. Hepatic fibrosis was induced by hypodermic injection of 40% CCI4. Recombinant AAV2-TGF β 3 viral particles were injected via the vena caudalis one week before CCh treatment. Rats were executed 8 weeks after CCI4 treatment, global histological change was observed after HE staining, the distribution of the collagen fibers was observed after masson staining, his-tochemistry was done to observe the expression of collagen Ⅰ; The positive area rate of the collagen fibers and the average optical rate of collagen Ⅰ were quantified. Results HE staining indicated that collagen fibers were reduced in the TGF β 3 group. Masson staining shown that the collagen fibers were distributed around the blood vessel, in the portal area and disse space. Compared to the model group (13.2%±2.2%) and negative control group (12.3%±1.5%), the collagen fibers in liver tissues of TGF β3 group (7.7% ± 1.5%) were significantly decreased (q = 9.456, P < 0.01; q = 8.217, P < 0.01). Histochemistry indicated that the collagen fibers of liver tissues of TGF 15 3 group (0.185±0.033) were significantly higher than those in the model group (0.252±0.042) and the negative control group (0.230±0.029), (q = 6.228, P < 0.01; q = 4.346, P < 0.01). Conclusion TGF β 3 alleviates the damage to hepatic cell and the level offibrosis in CCI4 treated rats and inhibits the expression of collagen Ⅰ.     

输血致盐水抗－D抗体1例

输血致盐水抗－Ｄ抗体１例广州器官移植配型中心肖露露，叶欣１９９４年４月，珠江医院转来１例盐水介质中交叉配血不合的疑难配血病例，经血型血清学检查证实为：Ｒｈ（Ｄ）阴性患者误输Ｒｈ（Ｄ）阳性血产生抗－Ｄ，现报告如下：１病历简介患者，男７２岁，胃癌术后。曾...     

婴儿维生素K缺乏的临床干预研究

目的探讨口服维生素K1对广西婴儿VKD的控制效果.方法3607例活产婴随机分为干预组1655例(出生～3月龄口服维生素K12.5mg共10次),对照组1952例(不用维生素K1),观察生后4个月内两组发生VKDB情况.对住院分娩的足月新生儿随机抽样分别口服维生素K12.5mg、5mg及不服药各60例,生后3天测定G-6-PD酶活性及观察黄疸、消化道、皮肤等反应.结果干预组无1例发生出血,对照组发生VKDB 11例(5.63%,P＜0.01).口服维生素K的足月新生儿对G-6-PD酶活性均无影响(P＞0.05),无黄疸加重、过敏及胃肠道不良等反应.结论婴儿小剂量10次口服维生素K1预防VKDB是简便、易行、经济、安全、有效的干预措施.     

经皮激光椎间盘减压术

经皮激光椎间盘减压术 (PercutaneousLaserdiscde compression ,PLDD)是继化学溶核术 (Chemenucleolysis)、经皮椎间盘切除术 (Percutaneousdiscectomy ,PD)、经皮自动椎间盘切除术 (Percutaneousautomateddiscectomy ,PAD)和关节镜下显微椎间盘切除术 (Athroscopicmicrodiscec     

桡侧屈腕肌移位至第三掌骨矫正痉挛性屈腕畸形

我院从1989年11月～1995年6月应用改良的腕屈肌移位代腕背伸肌矫正痉挛性腕屈曲畸形31例,经临床随访观察,疗效满意。1 临床资料 本组31例,男17例,女14例,年龄最小6岁,最大25岁,平均年龄17岁;左侧18例,右侧13例;术前腕关节均显屈曲畸形,不能伸直,持拿功能丧失,生活不能自理。     

多处软膜下横切术治疗外伤性癫痫8例

多处软膜下横切术治疗外伤性癫痫８例宋和顺王维兴钱涛肖福顺我院自１９９２年６月～１９９６年４月，采用多处软膜下横切术治疗外伤性癫痫８例，其中男５例，女３例。年龄１６～３４岁，平均２５岁。癫痫病程３～５年。脑挫裂伤５例，急性硬膜下血肿３例。癫痫发作频率５...     

病理学计算机试题库的研制

<正> 考试是教学过程的重要组成部分,也是检查学生学习质量,衡量教学效果的重要方法之一。为了进一步提高考试质量,使之走向规范化、科学化,我室全体教师从八九年三月开始,经过一年半的共同努力,与数学教研室协作,在学院率先研制建立病理学计算机试题库。经过两年的反复操作试验,可根据不同要求在很短时间内命出一份完整的病理学试卷(含标准答案及评分标准),基本上实现了命题、试卷编制及打印的自动化管理。现将试题库的建立及有关问题小结于下。