X线导向经皮椎弓根钉外固定治疗胸腰椎骨折187例临床应用

1995年2月～2002年4月,本组采用C型臂电视透视下、导向椎弓钉进入椎体并行外固定,对187例胸腰椎骨折手术,随访1～6年,疗效满意,现报告如下。1 资料和方法 一般资料:本组187例,男153例,女34例;年龄18～58岁。受伤原因:交通伤147例,跌落伤32例,塌方性压缩骨折8例。骨折部位:T_(12)51例,L_175例,L_227例,L_321例;胸腰段2个椎体6例,L_1和L_24例;胸腰段3个椎体3例。术前神经损伤分级:按美国脊髓损伤协会(ASIA)分级标准D级179例,C级8例。完全及不完全截瘫患者禁忌使用,临床骨折处分别有疼痛与神经症状。 仪器:采用日本岛津50N型C形臂X线机,MEDILAND P2000手术床,动态电视监控透视。     

人中穴在临床的应用体会

人中为临床常用穴,其不仅是急救要穴,对癫痫、面瘫、急性腰扭伤、落枕、脱肛、消化不良等病症亦有显著的治疗作用。     

农村盆腔瘀血综合征327例诊治体会

盆腔瘀血综合征与慢性盆腔病变有关,常伴有全身多系统功能紊乱,临床表现症状多,体征少,易误诊为神经官能症等疾病,门诊调查并诊治327例,现将有关资料报告如下: 1 临床资料 本组327例均为门诊育龄期患者,其中25岁至30岁98例,占29.97％,31岁至35岁115例,占35.2％,36岁至40岁79例、占23.2％,41岁至46岁35例,占10.7％,全年均有就诊,本     

国产11种金丝桃属植物中化学成分的含量分析

采用RP—HPLC梯度洗脱和等强度洗脱相结合的方法测定11种金丝桃届植物的提取物中5种化合物的含量。结果发现金丝桃属植物中各化学成分含量差异较大，其中贯叶金丝桃中的芦丁、金丝桃苷、金丝桃素含量均高于其他种；仅贯叶金丝桃、扬子小连翘、元宝草和遍地金等4种草本类中被检测出含有金丝桃素，而灌木类中未检测出；11种金丝桃属植物中都含有贯叶金丝桃素，且贯叶金丝桃素在展萼金丝桃中的含量高于贯叶金丝桃。这提示展萼金丝桃、扬子小连翘、元宝草和遍地金有望成为贯叶金丝桃的替代品。     

斑疹伤寒误诊为病毒性肝炎14例临床分析

我院自1994年1月～2002年12月共收治斑疹伤寒26例,其中误诊为病毒性肝炎14例,现分析如下. 1临床资料 一般资料:14例均为农民.其中男性10例,女性4例;年龄9～52岁.散发.患者生活环境卫生条件较差,有虱及老鼠接触史.     

党参的研究现状及开发

通过查阅文献和生产调查，概述了党参的功效、种类分布、生物学特性、病虫害情况及开发现状，并对党参的开发和栽培提出了建议。     

靶向Survivin的siRNA联合吉西他滨抑制胰腺癌细胞增殖

Objective To construct the siRNA eukaryotic expression vector targeting Survivin gene, and investigate the chemotherapy sensitivity of pancreatic cancer line Panc-1 treated by gemcitabine. Methods The siRNA eukaryotic expression vector targeting Survivin gene was constructed. Panc-1 cells were transfected with negative control vector or siRNA vector and selected by G418, and the cell growth curve was drawn. The expression of Survivin mRNA and protein was detected by RT-PCR and Western blot respectively. Panc-1 cells or transfected cells were treated with gemcitabine for 24 h,and then the growth inhibition rate was measured by MTT assay, and cell apeptosis rate was measured by flow cytometry. Results The result of endonuclease digestion and DNA sequencing revealed that the recombinant plasmid psiRNA-Survivin was constructed successfully. Survivin mRNA and protein levels were reduced by 79.2% and 83.6% respectively in stably transfected Panc-1 cells as compared with control group, and the cell growth curve was much smoother, and the growth inhibition rate [ ( 24.6±4.5 ) % / (38.7±5.2 ) % ] and apoptosis rate of these cells [ ( 16.7±2.5 ) %/( 26.8±3.4 ) % ] were significantly increased after treatment by gemcitabine (P < 0.05 ). Conclusion The constructed siRNA eukaryotie expression vector targeting Survivin could decrease the Survivin expression,inhibit the growth of Panc-1 cells significantly,and increase the chemotherapy sensitivity to gemcitabine.     

脑梗死患者凝血和纤溶机制的研究

目的　探讨脑梗死患者急性期、亚急性期和恢复期血浆纤维蛋白原、D -二聚体 (D -D)、组织型纤溶酶原激活物 (t -PA)和纤溶酶原 (PLG)含量的变化及意义。方法　对 60例脑梗死患者急性期、亚急性期和恢复期纤维蛋白原、D -D、t -PA、PLG的水平进行测定 ,并与 2 5例健康对照者进行比较。结果　纤维蛋白原和D -D的含量在 3阶段均高于对照组 (P <0 0 1) ,且升高的程度与梗死的体积有一定的关系 ;纤维蛋白原的含量在 3个阶段无明显统计学差异 (P >0 .0 5 ) ,而D -D在亚急性期显著升高 (P <0 .0 5 ) ,恢复期开始下降。T -PA和PLG于急性期时 ,t-PA血浆水平高于对照组 (P <0 .0 1) ,PLG则低于对照组 (P <0 .0 1)。结论　脑梗死患者在发病后存在凝血 -纤溶系统的紊乱