丙型肝炎病毒NS5a高免疫原区人工合成多肽抗原性的研究

目的 检测不同基因型丙型肝炎病毒(HCV)非结构5a区(NS5a)高免疫原区短链多肽的抗原性，确定该区的主要线性抗原表位并探讨基因异质性与免疫原性间的关系。方法 设计并合成特异性多肽；DNA Star软件对多肽氨基酸同源性进行比较；间接ELISA法检测多肽的抗原性。结果多肽氨基酸的同源性在不同区及不同基因型间变化较大。氨基酸残基2212～2241、2272-2301和2302-2331的多肽抗原性最强。18个来自保守区的多肽可以与不同基因型抗-HCV阳性血清起反应(阳性率高达96％)；而来自不同基因型间氨基酸序列保守性较低区的多肽抗原性与同基因血清的反应性较高。结论 HCV NS5a高免疫原区主要的线性抗原位于氨基酸残基2212-2241、2272-2301和2302-2331的位置；人工合成的多肽可以有效地用于检测抗-HCV抗体；一些多肽的抗原性具有基因型特异性。     

沈阳地区人体旋毛虫病156例临床分析

沈阳地区人体旋毛虫病１５６例临床分析石理兰，刘玉琢刘春兰（中国医科大学第二临床学院传染科）（沈阳二四五医院传染科）杨秀环（辽宁省邮电医院）关键词旋毛虫病，抗嗜酸性粒细胞血清，感染度，丙硫咪唑人体旋毛虫病近年来在沈阳地区常有发生，且有不断上升趋势。现将...     

HBV携带者血清病毒标志物和HBV DNA与肝组织中HBVcccDNA相关性的研究

Objective To investigate the correlation of sera HBV DNA and serological makers with hepatic tissue HBVcccDNA in chronic HBV carriers. Methods Real time fluorescence quantitative polymerase chain reaction (RT-PCR) were used to detect HBV covalently closed circular DNA (cccDNA) and total intrahepatic HBV DNA from 30 needle-biopsy specimens as well as HBV DNA in sera in chronic HBV carriers. Quantification of the HBsAg, HBeAg in sera were quantified using Chemiluminescence immunoassay. Results HBVcccDNA can be detected in chronic HBV carriers, which rang from 3.15 × 103 copies/mg to 1.06 × 107 copies/mg. There was a positive correlation between the cccDNA and HBVtDNA (r =0. 375, P < 0. 05 ), but there was no correlation between the cccDNA and sera HBV DNA (P =0. 174). There was a positive correlation between cccDNA and sera HBsAg quantification (r =0. 562, P <0. 001 ) but no correlation with sera HBeAg qantification ( r = 0. 152, P > 0. 05 ). Conclusion HBV cccDNA can be replicated stably in hepatic tissue in all chronic HBV carriers. HBV DNA in sera can not be indicated hepatic tissue cccDNA level. While HBsAg quantification in sera can be used as a marker of cccDNA quantification in hepatic tissue to some extent.