电针对脑缺血/再灌注损伤后大鼠海马CA1区神经元L型钙离子通道的调制作用

目的:试图阐明L型钙离子通道(L-VGCCS)在脑缺血/再灌注中的作用,并观察针刺对其的调制效应。方法:制作大鼠MCAO模型,应用荧光双标技术结合激光共聚焦显微镜,观察侧脑室定位注射L-VGCCS阻断剂及针刺对MCAO大鼠海马CA1区神经元细胞内Ca2+含量的影响。结果:侧脑室定位注射L-VGCCS阻断剂Nitrendpine对缺血/再灌注后的钙超载有明显的抑制作用,针刺并加药组[Ca2+]i与加药模型组相比显著降低,针刺治疗组对[Ca2+]i升高的抑制作用优于单纯阻断L-VGCCS通道的作用。结论:针刺治疗的作用机制可能是阻断L-VGCCS的功能,抑制钙超载,维持钙稳态,拮抗脑缺血/再灌注后继发神经元的损伤。     

CLINICAL AND EXPERIMENTAL RESEARCH ON APOPLECTIC SEQUELAE TREATED WITH “XINGNAO KAIQIAO”(XNKQ) ACUPUNCTURE METHOD

ApoplecticSequelaeisoneoftherefractorydiseaseswithlongdurationofillnessandrestora-tion,nervousfunctiondefectandhighermaimedrateinmedicalfieldnowaday,thusitseriouslyaffectsthelivingqualitycfpatientsandmakesveryheavyburdentofamilyandsociety.Wetreatedthisdiseasewitl1"XNKQ"acupuncturemethodcreatedbyProf.ShiXuemin,observedtheeffectsofclinicaIandexperimentalresearh,obtainedbetteroutcom,theresultswereasfol-lows:cLINICALmThRan1.Subjects:Sixtycases(in-patients)withapop1eticsequelaeweredividedint…     

以针灸治疗为中心的中风诊疗体系

针对中风病的基本病机为瘀血、肝风、痰浊等病理因素蒙蔽脑窍致“窍闭神匿,神不导气”,创立了“醒脑开窍”针刺法,选穴以阴经和督脉穴为主,并强调针刺手法量学规范,有别于传统的取穴和针刺方法。以“醒脑开窍”针刺法治疗为主,并根据病情需要辅以降颅压、抗感染、降血压之西药及支持疗法等,经对９００５例临床各期中风患者的治疗统计,痊愈率５９．２７％,显效率２３．１５％,总有效率９８．５６％。     

CLINICAL OBSERVATIONS ON ACUPUNCTURE TREATMENT OF PSEUDOBULBAR PALSY——A Report of 325 Cases

Pseudobulbar palsy,one of the maincomplications caused by cerebral apoplexy,results from supranuclear injury of motornuclei of the cranial nerve dominating the bulb.It is characterized by dysphagia,dysarthria,and abnormality of mental activity.In the past,the patients died generally of secondaryhypostatic pneumonia and serious malnutritiondue to lack of positive and initiative measures     

30 CASES OF “PULSELESS DISEASE” TREATED WITH ACUPUNCTURE

Since 1975 our hospital has admitted 30patients with“pulseless disease”and treated themwith acupuncture.Results have been satisfactory.The clinical summary is as follows.CLINICAL DATAThe 30 patients were treated between 1975and 1985.In-patients numbered 23;the other 7were out-patients.     

针灸"治神"的研究

"治神"是在针刺治病过程中极为重要的方法和要领."神"是人体生命活动的外在表现,如<素问·宝命全形论>曰:"凡刺之真,必先治神"."治神"是医患双方"神"志意念的交流过程,它是影响针灸疗效的关键因素.     

针刺量学和规范化研究中针灸仪器应用的思考

针刺手法定量化是针灸研究规范化的重要环节,但传统针刺手法操作复杂,难以定量化,通过应用针灸仪器达到定量化给针灸规范化研究提供了新的手段.应提高针灸界对研制针灸仪器必要性的认识,加强相关理论的研究,加强针刺测试仪和模拟仪器的研制,扩大使用范围,验证效能,发展针刺手法理论,丰富针灸规范化的研究方法,推进针灸国际化进程.     

醒脑开窍针刺法对缺血再灌注大鼠大脑皮层IL-1RI和TNFR-I的mRNA表达的影响

目的 探讨醒脑开窍针刺法干预缺血再灌注后脑组织炎症反应的分子机制.方法 以醒脑开窍针刺法为干预手段,以大鼠急性局灶性脑缺血再灌注模型作为研究对象,运用RT-PCR方法,观测不同时间点病变侧大脑皮层2种关键炎性细胞因子受体即白细胞介素-1受体Ⅰ(IL-1RⅠ)和肿瘤坏死因子受体Ⅰ(TNFR-Ⅰ)的mRNA表达情况,并观察针刺对上述指标的调节作用规律.结果 醒脑开窍针刺法降低了大鼠脑缺血再灌注后大脑皮层异常升高的IL-1RI、TNFR-Ⅰ的mRNA的表达,且该作用随时间的延长而更为明显.结论 醒脑开窍针刺法可干预缺血再灌注后脑组织炎症反应,进而对脑损伤起保护作用.     

ADVANCES IN CLINICAL AND EXPERIMENTAL STUDIES ON ACUPUNCTURE TREATMENT OF ACUTE CEREBRAL HEMORRHAGE

Acupuncturehasbeenwidelyusedfortreating cerebralhemorrhage(CH)atthestationary,recovery andsequelaestages,anditstherapeuticeffecthas beenconfirmedclinically.Withthegraduallydeep enedexperimentalstudiesonthemechanismsof acupunctureandtheincreaseofknowledgeabout cerebralphysiologyandpathology,experimentalre searchonclinicalapplicationofacupuncturetherapy foracuteCHhasbeenmadeatmultiplelevelsahead ofclinicalstudy.Inaddition,withtheexpansionof clinicalapplication,acupuncturetherapyhasbeenal readyu…     

调神益肾针法对更年期大鼠下丘脑阿片促黑素皮质素原mRNA表达的影响

目的：研究调神益肾针法对更年期雌性大鼠下丘脑阿片促黑素皮质素原mRNA表达的影响。方法：采用逆转录－聚合酶链反应技术。结果：更年期大鼠下丘脑阿片促黑素皮质素原mRNA表达明显降低；经针刺干预后，调神益肾组该基因的表达明显上调，补肾健脾组则无明显变化。结论：增强下丘脑阿片促黑素皮质素原mRNA的表达可能是调神益肾针法治疗更年期综合征的机制之一。