4.5Gyγ射线照射后比格犬凝血机制的改变

目的 比格犬4.5 Gy γ射线照射后,经综合对症和细胞因子2种治疗,观察其凝血机制的改变。方法 血小板体外聚集实验、血栓弹力图(TEG)描记分法、出凝血时间检测。结果 照后7 d照射对照组、综合对症组血小板聚集率明显降低,TEG凝固时间(k)值升高,而细胞因子治疗组则变化不明显。照射后14 d照射对照组TEG各项参数变化最为明显,表现为凝血反应时间(r)、凝固时间(k)、r+k和血栓最大凝固时间(M)值大幅度升高,凝血时间、血栓最大凝固时间延迟,血栓最大弹力度(Ma)值大幅度下降,血栓最大弹力幅度减小。细胞因子治疗组各项参数均好于综合对症组。照射对照组照射后14 d凝血酶时间明显延长,综合对症和细胞因子组于照后2~3周凝血酶时间最长,两组间差异无统计学意义。照后42 d,血小板体外聚集实验综合对症组75%动物血小板聚集功能处于极低水平(正常值的20%以下),而细胞因子对症组仅有33%动物血小板聚集功能处于极低水平。结论 细胞因子新组合治疗可明显改善急性放射病比格犬血小板聚集和凝血功能。     

紫外分光光度法测定呱西替柳胶囊中哌西替柳的含量

采用紫外分光光度法测定呱西替柳胶囊中呱西替柳的含量。当呱西替柳的甲醇溶液在浓度为１５－５０μｇ／ｍｌ时，与吸收度呈很好的线性关系（ｒ＝０．９９９８），平均回收率为１０１．０％，ＲＳＤ为０．８８％（ｎ＝６）。该方法简单，快速、结果满意。     

单纯肥胖儿童的智能发育研究

调查苏州市的2所幼儿园中,肥胖儿童检出率为2.68%(19/710)。经1∶1病例对照研究,采用图片词汇测试法,两组儿童的智商无明显差异,而平均时间(秒/张),肥胖组显著长于对照组,提示肥胖儿童的思维敏捷性可能处于劣势。     

胎膜早破与新生儿感染的相关性研究

目的分析足月胎膜早破产妇阴道分泌物的病原体分布特点及与新生儿感染发生率的关系。方法回顾性分析2014年8月-2015年8月医院产科住院分娩产妇的临床资料,92例足月胎膜早破产妇为病例组;88例怀疑有胎膜早破产妇,设为对照组Ⅰ;85例产妇为在医院进行产前检查的健康女性,设为对照组Ⅱ。对比三组产妇阴道分泌物病原体分布特点、阴道分泌物免疫调节因子水平、产妇及新生儿感染发生率。结果病例组病原体分布特点不同于对照组Ⅰ和对照组Ⅱ,病例组乳杆菌属检出率(46.74%)低于对照组Ⅰ(70.45%)与对照组Ⅱ(83.53%),病例组大肠埃希菌检出率(54.35%)高于对照组Ⅰ(31.82%)与对照组Ⅱ(31.76%),差异有统计学意义(P0.05)。病例组产妇阴道分泌物中白细胞介素6(IL-6)(518.23±73.49)ng/mL、肿瘤坏死因子α(TNF-α)水平(12.93±1.57)fmol/mL高于对照组Ⅰ(321.64±75.94)ng/mL、(9.22±1.34)fmol/mL与对照组Ⅱ(257.42±66.35)ng/mL、(8.71±1.08)fmol/mL,病例组产妇阴道分泌物中白细胞介素2(IL-2)水平(1.57±0.68)ng/mL低于对照组Ⅰ(1.83±0.62)ng/mL与对照组Ⅱ(1.91±0.44)ng/mL,差异有统计学意义(P0.05)。病例组产妇感染率(7.61%)、新生儿感染率(6.52%)高于对照组Ⅰ与对照组Ⅱ,差异有统计学意义(P0.05)。结论胎膜早破对母婴健康造成严重影响,足月胎膜早破导致产妇及新生儿感染率升高,阴道菌群失调是引起感染增加的主要因素,应积极给予预防处理,防止因菌群失调而发生胎膜早破,降低产妇及新生儿感染发生率。     

无血清培养基对树突细胞体外诱导的影响

我们比较了无血清培养基X—VIVO 20与有血清培养基(含10％人AB血清或胎牛血清RPMI 1640)对外周血单核细胞来源的树突细胞(DC)体外质导、分化成熟的影响。     

生化汤加减联合针刺治疗剖宫产术后子宫复旧不良49例临床观察

产后血性恶露持续3周以上仍淋漓不断者称为恶露不绝,西医称为子宫复旧不良。近年随着剖宫产率升高,产后子宫复旧不良病例明显增多,常导致产后贫血、晚期产后出血、继发不孕、月经失调及盆腔感染等,严重影响妇女身心健康。对于产后子宫复旧不良,西医多采用缩宫素加抗生素等对症治疗,效果欠佳,且抗生素的副作用及对母乳喂养的影响使其应用受到限制。2011—10—2012-10,我们采用生化汤联合针刺治疗剖宫产术后子宫复旧不良49例,并与缩宫素治疗49例对照观察,结果如下。     

妊娠38周宫颈剥膜诱导临产效果观察

目的探讨妊娠38周孕妇行宫颈剥膜诱导自然临产的可行性及意义。方法将200例妊娠38周的初产妇随机分为观察组和对照组各100例。观察组行宫颈扩张并剥膜,之后每周检查一次至临产或自然破膜,对照组不采取干预措施,自然待产。比较两组自然临产率、孕周、分娩方式,观察两组新生儿窘迫发生率及体质量。结果与对照组比较,观察组自然临产率高、经阴分娩率高,孕周短（P均〈0．05）。观察组新生儿窘迫发生率及体质量均明显低于对照组（P均〈0．05）。结论妊娠38周宫颈剥膜诱导自然临产效果明显,经阴分娩率高。     

中药内服外敷合黑光疗法治疗带状疱疹102例的观察及护理

带状疱疹又名“缠腰火丹”。我院2006年5月-2010年5月应用中药内服外敷合黑光疗法治疗带状疱疹102例,疗效显著,现报道如下。一般资料 102例均为门诊病人,其中男60例,女42例;年龄20-80岁;病程最短l天,最长30天。治疗方法内服龙胆泻肝汤加减：龙胆草9g,栀子10g,柴胡10g,黄芩20g,泽泻12g,生地黄12g,生甘草6g,当归12g,地龙15g,豨莶草15g,徐长卿15g,川楝子10g。     

对选择性多巴胺和去甲肾上腺素再摄取抑制剂Solriamfetol的研究进展

选择性多巴胺和去甲肾上腺素再摄取系统抑制剂索里亚姆菲托(Solriamfetol)是临床上治疗成人帕金森病、阻塞性睡眠呼吸暂停、发作性睡病所致白天过度嗜睡的常用药.此药是空中生物制药公司研制的产品,是一种口服制剂,具有强大的唤醒促进作用.此药是FDA批准的用于治疗发作性睡病、阻塞性睡眠呼吸暂停所致白天过度嗜睡的首个具有...     

Studies on mechanism of treatment of granulocyte colony-stimulating factor,recombinant human interleukin-11 and recombinant human interleukin-2 on hematopoietic injuries induced by 4.5 Gy γ-rays irradiation in beagles

Objective To investigate the mechanism of treatment of granulocyte colony-stimulating factor(rhG-CSF),recombinant human interleukin-11(rhIL-11)and recombinant human interleukin-2 (rhIL-2)on hematopoietic injuries induced by 4.5 Gy60 Coγ-ray irradiation in beagles,and to provide experimental evidence for the clinical treatment of extremely severe myeloid acute radiation sickness (ARS).Methods Sixteen beagle dogs were given 4.5 Gy60 Co γ-ray total body irradiation(TBI),then randomly assigned into irradiation control group,supportive care group or cytokines+supportive care (abbreviated as cytokines)group.In addition to supportive care,rhG-CSF,rhlL-11 and rhIL-2 were administered subcutaneously to treat dogs in cytokines group.The percentage of CD34+cells,cell cycle and apoptosis of nucleated cells in peripheral blood were examined by Flow cytometry.Results After 4.5 Gy 60 Co γ-ray irradiation,the CD34+cells in peripheral blood declined obviously(61.3%and 52.1% of baseline for irradiation control and supportive care group separately).The cell proportion of nucleated cells in Go/G1 phase was increased notably(99.27% and 99.49% respectively).The rate of apoptosis(26.93% and 21.29% separately)and necrosis(3.27% and 4.14%,respectively)of nucleated cells were elevated significantly when compared with values before irradiation(P<0.05) 1 d post irradiation.When beagles were treated with cytokines and supportive care,the CD34+cells in peripheral blood were markedly increased(135.6% of baseline).The effect of G0/G1 phase blockage of nucleated cells became more serious(99.71%).The rate of apoptosis(5.66%)and necrosis(1.60%)of nucleated cells were significantly lower than that of irradiation control and supportive care groups 1 d after exposure.Conclusions Cytokines maybe mobilize CD34+cells in bone marrow to peripheral blood,indce cell cycle block at G0/G1 phase and reduce apoptosis,and eventually cure hematopoieticinjuries induced by irradiation.